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Cited by 46Renal function and acid-base balanceBone Metabolism and DiseasesKidney Stones and Urolithiasis Treatments

Effect of Metabolic Acidosis on the Potassium Content of Bone

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Bushinsky, David A., Gavrilov, Konstantin, Chabala, Jan M., Featherstone, John D. B., Levi-Setti, Riccardo·1997·Journal of Bone and Mineral Research
DOI 10.1359/jbmr.1997.12.10.1664
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Abstract

Metabolic acidosis induces resorption of cultured bone, resulting in a net efflux of calcium (Ca) from the bone and an apparent loss of mineral potassium (K). However, in these organ cultures, there is diffusion of K between the medium and the crystal lattice, causing difficulty in interpretation of the acid-induced changes in mineral ion composition. To determine the effects of acidosis on bone mineral K, we injected 4-day-old neonatal mice with pure stable isotope41K, equal to ∼5% of their total body K. Calvariae were dissected 24 h later and then cultured for 24 h in medium without added41K, either at pH ∼7.4 (Ctl) or at pH ∼7.1 (Ac), with or without the osteoclastic inhibitor calcitonin (3 × 10−9 M, CT). The bone isotopic ion content was determined with a high-resolution scanning ion microprobe utilizing secondary ion mass spectrometry.41K is present in nature at 6.7% of total K. The injected41K raised the ratio of bone41K/(39K+41K) to 9.8 ± 0.5% on the surface (ratios of counts per second of detected secondary ions, mean ±95% confidence interval) but did not alter the ratio in the interior (6.9 ± 0.4%), indicating biological incorporation of the41K into the mineral surface. The ratios of41K/40Ca on the surface of Ctl calvariae was 14.4 ± 1.2, indicating that bone mineral surface is rich in K compared with Ca. Compared with Ctl, Ac caused a marked increase in the net Ca efflux from bone that was blocked by CT. Ac also induced a marked fall in the ratio of41K/40Ca on the surface of the calvariae (4.3 ± 0.5, p < 0.01 vs. Ctl), which was partially blocked by CT (8.2 ± 0.9, p < 0.01 vs. Ctl and vs. Ac), indicating that Ac causes a greater release of bone mineral K than Ca which is partially blocked by CT. Thus, bone mineral surface is rich in K relative to Ca, acidosis induces a greater release of surface mineral K than Ca, and osteoclastic function is necessary to support the enriched levels of surface mineral K in the presence of acidosis.

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  • BEF has a different ionic composition from systemic extracellular fluid (SEF) and establishes an extensive contact surface (215 m2, 120-fold the size of the trabecular network) with the mineralized matrix to allow efficient, short-term mineral exchange with SEF.

    “BEF has a different ionic composition from systemic extracellular fluid (SEF)22 and establishes an extensive contact surface (215 m2, which is 120-fold the size of the trabecular network) with the mineralized matrix to allow efficient, short-term mineral exchange with SEF.23–26”

    From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [24]

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Research works printed but not linked: 2

  1. Widdowson EM, Dickerson JWT 1964 Chemical composition of the body. In: Comar CL, Bronner F (eds.) Mineral Metab- olism. Academic Press, Inc., New York, NY, U.S.A. pp. 1-247.
  2. BUSHINSKY ET AL. 15234681, 1997, 10, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1359/jbmr.1997.12.10.1664 by Readcube (Labtiva Inc.), Wiley Online Library on [11/06/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License behavior of carbonated apatites prepared at controlled pH and temperature. J Colloid Interface Sci 130:467-479. · DOI

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Authors
Bushinsky, D. A., Gavrilov, K., Chabala, J. M., Featherstone, J. D. B., & Levi-Setti, R.
Affiliations
  • University of Rochester Medicine (US)
  • University of Chicago (US)
  • University of California, San Francisco (US)
  • Enrico Fermi Institute, Department of Physics, University of Chicago, Chicago, Illinois, U.S.A.
Corresponding author
Bushinsky, David A.
ORCID
Venue
Journal of Bone and Mineral Research
Publisher
Oxford University Press (OUP)
Published
1997-10-01
Volume
12
Issue
10
Pages
1664-1671
ISSN
  • 0884-0431 (ISSN-L)
  • 1523-4681
DOI
10.1359/jbmr.1997.12.10.1664
OpenAlex
W2078948216
Cited by
46
References
36
Citations by year
2026: 1 · 2024: 3 · 2023: 1 · 2022: 3 · 2020: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
2.91
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Journal of Bone and Mineral Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p50dk047631-05, 5r01ar039906-08, AR 39906, AR 39906 and DK 47631, DK 47631)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-47631)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 39906)
Topics
  • Health Sciences › Medicine › Nephrology › Renal function and acid-base balance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Kidney Stones and Urolithiasis Treatments
Keywords
metabolic acidosis, bone resorption, osteoclasts, calcitonin, calcium efflux, calvaria, bone mineral composition, neonatal mice, potassium/calcium ratio
MeSH terms
Acidosis, Animals, Animals, Newborn, Calcitonin, Calcium, Frontal Bone, Hydrogen-Ion Concentration, Isotope Labeling, Microscopy, Electron, Scanning, Organ Culture Techniques, Osteoclasts, Parietal Bone, Potassium, Potassium Isotopes, Skull, Mass Spectrometry, Bone Density, Mice

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