The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens
Open accessAbstract
Functional two-photon Ca(2+)-imaging is a versatile tool to study the dynamics of neuronal populations in brain slices and living animals. However, population imaging is typically restricted to a single two-dimensional image plane. By introducing an electrically tunable lens into the excitation path of a two-photon microscope we were able to realize fast axial focus shifts within 15 ms. The maximum axial scan range was 0.7 mm employing a 40x NA0.8 water immersion objective, plenty for typically required ranges of 0.2-0.3 mm. By combining the axial scanning method with 2D acousto-optic frame scanning and random-access scanning, we measured neuronal population activity of about 40 neurons across two imaging planes separated by 40 μm and achieved scan rates up to 20-30 Hz. The method presented is easily applicable and allows upgrading of existing two-photon microscopes for fast 3D scanning.
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Citations
Cites
Deep tissue two-photon microscopy
Helmchen, F., & Denk, W. (2005) · Nature Methods · doi:10.1038/nmeth818 · Cited by 4,153
Self-citationIn vivo two-photon calcium imaging of neuronal networks
Stosiek, C., Garaschuk, O., Holthoff, K., & Konnerth, A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1232232100 · Cited by 1,312
Variable focal lens controlled by an external voltage: An application of electrowetting
Berge, B., & Peseux, J. (2000) · The European Physical Journal E · doi:10.1007/s101890070029 · Cited by 1,074
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Nimmerjahn, A., Kirchhoff, F., Kerr, J. N. D., & Helmchen, F. (2004) · Nature Methods · doi:10.1038/nmeth706 · Cited by 784
Self-citationBroadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex
Kerlin, A. M., Andermann, M. L., Berezovskii, V. K., & Reid, R. C. (2010) · Neuron · doi:10.1016/j.neuron.2010.08.002 · Cited by 613
High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision
Grewe, B. F., Langer, D., Kasper, H., Kampa, B. M., & Helmchen, F. (2010) · Nature Methods · doi:10.1038/nmeth.1453 · Cited by 522
Self-citationImaging cellular network dynamics in three dimensions using fast 3D laser scanning
Göbel, W., Kampa, B. M., & Helmchen, F. (2007) · Nature Methods · doi:10.1038/nmeth989 · Cited by 365
Self-citationImaging in vivo: watching the brain in action
Kerr, J. N. D., & Denk, W. (2008) · Nature Reviews Neuroscience · doi:10.1038/nrn2338 · Cited by 346
Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity
Duemani Reddy, G., Kelleher, K., Fink, R., & Saggau, P. (2008) · Nature Neuroscience · doi:10.1038/nn.2116 · Cited by 340
Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
Cheng, A., Gonçalves, J. T., Golshani, P., Arisaka, K., & Portera-Cailliau, C. (2011) · Nature Methods · doi:10.1038/nmeth.1552 · Cited by 289
Cited by
In vivo imaging of neural activity
Yang, W., & Yuste, R. (2017) · Nature Methods · doi:10.1038/nmeth.4230 · Cited by 406
Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain
Stirman, J. N., Smith, I. T., Kudenov, M. W., & Smith, S. L. (2016) · Nature Biotechnology · doi:10.1038/nbt.3594 · Cited by 331
dal Maschio, M., Donovan, J. C., Helmbrecht, T. O., & Baier, H. (2017) · Neuron · doi:10.1016/j.neuron.2017.04.034 · Cited by 169
Metadata
- Authors
- Grewe, B. F., Voigt, F. F., van ’t Hoff, M., & Helmchen, F.
- Venue
- Biomedical Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2011-06-23
- Volume
- 2
- Issue
- 7
- Pages
- 2035
- ISSN
- 2156-7085 (ISSN-L)
- OpenAlex
- W2003429530
- Cited by
- 310
- References
- 16
- FWCI
- 7.98
- Type
- journal article
- Language
- en
- Open access
- gold
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- Grewe, B. F., Voigt, F. F., van ’t Hoff, M., & Helmchen, F. (2011). Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens. Biomedical Optics Express. https://doi.org/10.1364/boe.2.002035
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