The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Resolution scaling in STED microscopy
Open accessAbstract
We undertake a comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal depletion patterns. We used an experimental platform featuring a high quality depletion pattern which results in operation close to the optimal optical performance. Its superior image brightness and uniform effective resolution <25 nm are evidenced by imaging both isolated and self-organized convectively assembled fluorescent beads. For relevant saturation values, the generalized square-root law is shown to predict the practical resolution with high accuracy.
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Cites
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006) · Science · doi:10.1126/science.1127344 · Cited by 8,772
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Rust, M. J., Bates, M., & Zhuang, X. (2006) · Nature Methods · doi:10.1038/nmeth929 · Cited by 8,078
Hell, S. W., & Wichmann, J. (1994) · Optics Letters · doi:10.1364/ol.19.000780 · Cited by 6,065
Self-citationUltra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
Hess, S. T., Girirajan, T. P. K., & Mason, M. D. (2006) · Biophysical Journal · doi:10.1529/biophysj.106.091116 · Cited by 3,605
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Self-citationGustafsson, M. G. L. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406877102 · Cited by 2,224
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
Willig, K. I., Rizzoli, S. O., Westphal, V., Jahn, R., & Hell, S. W. (2006) · Nature · doi:10.1038/nature04592 · Cited by 1,225
Self-citationWide-field subdiffraction imaging by accumulated binding of diffusing probes
Sharonov, A., & Hochstrasser, R. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609643104 · Cited by 1,151
Mechanism of formation of two-dimensional crystals from latex particles on substrates
Denkov, N., Velev, O., Kralchevski, P., Ivanov, I., Yoshimura, H., & Nagayama, K. (1992) · Langmuir · doi:10.1021/la00048a054 · Cited by 1,136
Hell, S. W. (2003) · Nature Biotechnology · doi:10.1038/nbt895 · Cited by 1,003
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Cited by
Two-photon excitation STED microscopy
Moneron, G., & Hell, S. W. (2009) · Optics Express · doi:10.1364/oe.17.014567 · Cited by 238
Self-citationWurm, C. A., Neumann, D., Lauterbach, M. A., Harke, B., Egner, A., Hell, S. W., & Jakobs, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1107553108 · Cited by 173
Self-citationLive-cell superresolution imaging by pulsed STED two-photon excitation microscopy
Takasaki, K. T., Ding, J. B., & Sabatini, B. L. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2012.12.053 · Cited by 142
Metadata
- Authors
- Harke, B., Keller, J., Ullal, C. K., Westphal, V., Schönle, A., & Hell, S. W.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2008-03-12
- Volume
- 16
- Issue
- 6
- Pages
- 4154
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2034951110
- Cited by
- 438
- References
- 22
- FWCI
- 12.98
- Type
- journal article
- Language
- en
- Open access
- gold
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- Harke, B., Keller, J., Ullal, C. K., Westphal, V., Schönle, A., & Hell, S. W. (2008). Resolution scaling in STED microscopy. Optics Express. https://doi.org/10.1364/oe.16.004154
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
