The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images
Open accessAbstract
A new global analysis algorithm to analyse (hyper-) spectral images is presented. It is based on the phasor representation that has been demonstrated to be very powerful for the analysis of lifetime imaging data. In spectral phasor analysis the fluorescence spectrum of each pixel in the image is Fourier transformed. Next, the real and imaginary components of the first harmonic of the transform are employed as X and Y coordinates in a scatter (spectral phasor) plot. Importantly, the spectral phasor representation allows for rapid (real time) semi-blind spectral unmixing of up to three components in the image. This is demonstrated on slides with fixed cells containing three fluorescent labels. In addition the method is used to analyse autofluorescence of cells in a fresh grass blade. It is shown that the spectral phasor approach is compatible with spectral imaging data recorded with a low number of spectral channels.
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Cites
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Cited by
Annibale, P., & Gratton, E. (2015) · Scientific Reports · doi:10.1038/srep09258 · Cited by 24
Metadata
- Authors
- Fereidouni, F., Bader, A. N., & Gerritsen, H. C.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2012-05-22
- Volume
- 20
- Issue
- 12
- Pages
- 12729
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2133362850
- Cited by
- 257
- References
- 14
- FWCI
- 6.19
- Type
- journal article
- Language
- en
- Open access
- gold
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- Fereidouni, F., Bader, A. N., & Gerritsen, H. C. (2012). Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images. Optics Express. https://doi.org/10.1364/oe.20.012729
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