The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes
Open accessAbstract
The influence of femtosecond near-infrared (NIR) microirradiation on cell vitality and cellular reproduction has been studied. Chinese hamster ovary cells exposed to a highly focused 150-fs scanning beam at 730, 760, and 800 nm (80 MHz, 80-mus pixel dwell time) of </=1 mW remained unaffected by the femtosecond microbeam. However, increased mean power led to impaired cell division. At >/=6 -mW mean power, cells were unable to form clones. They died or became giant cells. Complete cell destruction, including cell fragmentation, occurred at mean powers >10 mW. Cell death was accompanied by intense luminescence in the mitochondrial region. When we consider the diffraction-limited spot size in the submicrometer region, intensities and photon flux densities of 0.8-kW pulses (10-mW mean power) are of the order of terawatts per square centimeter (10(12)W/cm (2)) and 10(32) photons cm(-2) s(-1) , respectively. Extremely high fields may induce destructive intracellular plasma formation. The power limitations should be considered during NIR femtosecond microscopy of vital cells and in the design of compact NIR femtosecond solid-state lasers for two-photon microscopes.
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Citations
Cites
Two-photon laser scanning fluorescence microscopy
Denk, W., Strickler, J. H., & Webb, W. W. (1990) · Science · doi:10.1126/science.2321027 · Cited by 9,488
Breakdown threshold and plasma formation in femtosecond laser–solid interaction
von der Linde, D., & Schüler, H. (1996) · Journal of the Optical Society of America B · doi:10.1364/josab.13.000216 · Cited by 308
Cell damage by near-IR microbeams
König, K., Liang, H., Berns, M. W., & Tromberg, B. J. (1995) · Nature · doi:10.1038/377020a0 · Cited by 170
Self-citationCell damage in near-infrared multimode optical traps as a result of multiphoton absorption
König, K., Liang, H., Berns, M. W., & Tromberg, B. J. (1996) · Optics Letters · doi:10.1364/ol.21.001090 · Cited by 158
Self-citationTime-resolved fluorescence microscopy using two-photon excitation
So, P. T. C., French, T. E., Yu, W., Berland, K. M., Dong, C. Y., & Gratton, E. (1995) · Bioimaging · doi:10.1002/1361-6374(199506)3:2<49::aid-bio1>3.0.co;2-e · Cited by 149
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Cited by
Multiphoton microscopy in life sciences
König, K. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00738.x · Cited by 1,265
Self-citationHighly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy
Hopt, A., & Neher, E. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)76173-5 · Cited by 442
Single Cell Optical Imaging and Spectroscopy
Stender, A. S., Marchuk, K., Liu, C., Sander, S., Meyer, M. W., Smith, E. A., Neupane, B., Wang, G., Li, J., Cheng, J.-X., et al. (2013) · Chemical Reviews · doi:10.1021/cr300336e · Cited by 292
Surgical applications of femtosecond lasers
Chung, S. H., & Mazur, E. (2009) · Journal of Biophotonics · doi:10.1002/jbio.200910053 · Cited by 281
König, K., Becker, T. W., Fischer, P., Riemann, I., & Halbhuber, K.-J. (1999) · Optics Letters · doi:10.1364/ol.24.000113 · Cited by 259
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Metadata
- Authors
- König, K., So, P. T. C., Mantulin, W. W., & Gratton, E.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 1997-01-15
- Volume
- 22
- Issue
- 2
- Pages
- 135
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2050144544
- Cited by
- 254
- References
- 5
- FWCI
- 7.02
- Type
- journal article
- Language
- en
- Open access
- green
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- König, K., So, P. T. C., Mantulin, W. W., & Gratton, E. (1997). Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes. Optics Letters. https://doi.org/10.1364/ol.22.000135
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
