Rapid determination of the three-dimensional orientation of single molecules
Abstract
A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.
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For a linear dipole emitter such as a single molecule, the polarization pattern of the emitted photons depends on the orientation of the dipole moment.
“For example, for a linear dipole emitter such as a single molecule, the polarization pattern of the emitted photons depends on the orientation of the dipole moment (Fourkas 2001, Novotny et al 2001).”
From Single-photon sources (2005) · cites this paper as Fourkas 2001
If the dipole is perpendicular to the focal plane, the polarization in the collected mode is radial.
“If the dipole is perpendicular to the focal plane, the polarization in the collected mode is radial (Fourkas 2001).”
From Single-photon sources (2005) · cites this paper as Fourkas 2001
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Lounis, B., & Orrit, M. (2005) · Reports on Progress in Physics · doi:10.1088/0034-4885/68/5/r04 · Cited by 908 · see citing works
Single-molecule spectroscopy and imaging over the decades
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- Authors
- Fourkas, J. T.
- Affiliations
- Boston College (US)
- Corresponding author
- Fourkas, John T.
- ORCID
- Fourkas, John T.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 2001-02-15
- Volume
- 26
- Issue
- 4
- Pages
- 211
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2071000865
- Cited by
- 167
- References
- 14
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 9 · 2023: 5 · 2022: 7 · 2021: 8 · 2020: 2 · 2019: 7 · 2018: 7 · 2017: 8 · 2016: 6 · 2015: 7 · 2014: 7 · 2013: 8 · 2012: 10
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Optics Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Engineering › Biomedical Engineering › Optical Polarization and Ellipsometry
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Keywords
- single-molecule orientation, transition dipole moment orientation, three-dimensional orientation, high numerical aperture objective, single-molecule fluorescence
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