Fourier Transform Infrared Microspectroscopy as a Tool to Differentiate Nitzschia Closterium and Nitzschia longissima
Abstract
Nitzschia closterium and Nitzschia longissima are two species of diatom that are extremely difficult to differentiate using light microscopy. This paper details an investigation into the use of FT-IR microscopy combined with discriminant analysis to differentiate between these species. Spectra were taken from unidentified samples and classified against a training set using either Mahalanobis distances or principal component analysis combined with canonical discriminant analysis. Unidentified samples were classified with a 100% accuracy using both mathematical techniques.
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FTIR with multivariate analysis has been used successfully to classify species and to differentiate the nutritional status of microalgae.
“The application of FTIR to classify species with the help of multivariate analysis [24 -29] and to differentiate the nutritional status of microalgae [30 -34] have been attempted successfully.”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [24]
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- Authors
- Vardy, S., & Uwins, P.
- Affiliations
- The University of Queensland (AU)
- ORCID
- Vardy, Suzanne
- Venue
- Applied Spectroscopy
- Publisher
- SAGE Publications
- Published
- 2002-12
- Volume
- 56
- Issue
- 12
- Pages
- 1545-1548
- ISSN
- 0003-7028 (ISSN-L)
- 1943-3530
- OpenAlex
- W2049200007
- Cited by
- 10
- References
- 12
- Citations by year
- 2021: 2 · 2019: 1 · 2014: 2 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Applied Spectroscopy (publishedVersion)
- HAL (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Materials Science › Biomaterials › Diatoms and Algae Research
- Keywords
- Nitzschia closterium, Nitzschia longissima, diatoms, species identification, Fourier transform infrared microspectroscopy, discriminant analysis, Mahalanobis distance, principal component analysis, canonical discriminant analysis
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