Differentiation of Algae Clones on the Basis of Resonance Raman Spectra Excited by Visible Light
Open accessAbstract
Fourteen algae clones belonging to four different classes, including clones of Pseudo-nitzschia (Bacillariophyceae), some of which are capable of producing the toxin domoic acid, have been studied by means of resonance Raman spectra excited at 457.9 and 488 nm. Spectra taken at both excitation wavelengths are of high quality and are sufficiently distinct to differentiate clones at the algal class level. All spectra contain major features near 1000, 1153, and 1523 cm(-)(1), which are strongly resonance enhanced due to carotenoid pigments. Weaker features between 920-980 and 1170-1230 cm(-)(1), also due to carotenoid pigments, are more characteristic of the algae clones and more directly reflect different carotenoid composition. Similarities and differences among spectra have been analyzed by the method of principal component analysis (PCA). A distinct clustering of spectral data according to algal class has been shown by PCA score plots. All Pseudo-nitzschia clones can be separated from other classes of algae on the basis of spectra, but it is not possible to distinguish toxic Pseudo-nitzschia from nontoxic clones on the basis of these spectra, which reflect only differences in carotenoid composition.
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The prominent carotenoid signal is not necessarily indicative of high carotenoid abundance because carotenoids contain delocalized π-electron systems, resulting in resonant excitation and strong amplification of the Raman signal.
“it should be noted that the prominent carotenoid signal is not necessarily indicative of high carotenoid abundance, because carotenoids contain delocalized π-electron systems (conjugated C = C double bonds), resulting in a resonant excitation and thus strong amplification of the Raman signal30.”
From Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy (2016) · cites this paper as [30]
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- Authors
- Wu, Q., Nelson, W. H., Hargraves, P., Zhang, J., Brown, C. W., & Seelenbinder, J. A.
- Affiliations
- University of Rhode Island (US)
- ORCID
- Brown, C. W.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1998-04-03
- Volume
- 70
- Issue
- 9
- Pages
- 1782-1787
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2046783048
- Cited by
- 41
- References
- 19
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Journal of Media Literacy Education (submittedVersion, cc-by)
- Funders
- National Science Foundation
- U.S. Department of Commerce
- National Oceanic and Atmospheric Administration
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Environmental Science › Environmental Chemistry › Marine Toxins and Detection Methods
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- resonance Raman spectroscopy, Pseudo-nitzschia, domoic acid, carotenoids, algal taxonomy, visible light excitation, diatoms, spectral clustering
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