Fine-scale mapping of natural variation in fly fecundity identifies neuronal domain of expression and function of an aquaporin
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To gain insight into the molecular genetic basis of standing variation in fitness related traits, we identify a novel factor that regulates the molecular and physiological basis of natural variation in female Drosophila melanogaster fecundity. Genetic variation in female fecundity in flies derived from a wild orchard population is heritable and largely independent of other measured life history traits. We map a portion of this variation to a single QTL and then use deficiency mapping to further refine this QTL to 5 candidate genes. Ubiquitous expression of RNAi against only one of these genes, an aquaporin encoded by Drip, reduces fecundity. Within our mapping population Drip mRNA level in the head, but not other tissues, is positively correlated with fecundity. We localize Drip expression to a small population of corazonin producing neurons located in the dorsolateral posterior compartments of the protocerebrum. Expression of Drip-RNAi using both the pan-neuronal ELAV-Gal4 and the Crz-Gal4 drivers reduces fecundity. Low-fecundity RILs have decreased Crz expression and increased expression of pale, the enzyme encoding the rate-limiting step in the production of dopamine, a modulator of insect life histories. Taken together these data suggest that natural variation in Drip expression in the corazonin producing neurons contributes to standing variation in fitness by altering the concentration of two neurohormones.
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CRZ signaling coordinates aspects of copulation and fecundity in Drosophila and steroid synthesis in Octopus gonads.
“For instance, CRZ signaling coordinates aspects of copulation and fecundity in Drosophila (29, 30) and steroid synthesis in Octopus gonads (31).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [30]
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- Authors
- Bergland, A. O., Chae, H.-S., Kim, Y.-J., & Tatar, M.
- Affiliations
- Brown University (US)
- Gwangju Institute of Science and Technology (KR)
- Corresponding author
- Bergland, Alan O.
- Chae, Hyo-seok
- Kim, Young-Joon
- Tatar, Marc
- Venue
- PLoS Genetics
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-04-05
- Volume
- 8
- Issue
- 4
- Pages
- e1002631
- ISSN
- 1553-7390 (ISSN-L)
- 1553-7404
- PMC
- PMC3320613
- OpenAlex
- W1964698454
- Cited by
- 35
- References
- 66
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 1
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS Genetics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- National Science Foundation (0215393, 0554548)
- U.S. Department of Agriculture (2002-34438-12688, 2003‐34438‐13111, 2008-34438-19246)
- National Research Foundation
- National Institutes of Health
- National Institute on Aging (R01AG031152, AG030329, R01AG030329, AG031152)
- National Institute of General Medical Sciences (F32GM097837)
- Office of Experimental Program to Stimulate Competitive Research (0554548)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Animal Behavior and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- female fecundity, natural variation, Drosophila melanogaster, aquaporins, DRIP, protocerebrum, standing genetic variation, quantitative trait loci, pale, dopamine synthesis, neurohormones
- MeSH terms
- Animals, Chromosome Mapping, Drosophila melanogaster, Female, Fertility, Gene Expression Regulation, Neurons, Neuropeptides, RNA, Messenger, Transcription Factors, Aquaporins, Drosophila Proteins, RNA Interference, Quantitative Trait Loci, Genetic Fitness
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