Identified peptidergic neurons in the Drosophila brain regulate insulin-producing cells, stress responses and metabolism by coexpressed short neuropeptide F and corazonin
Open accessAbstract
Insulin/IGF-like signaling regulates the development, growth, fecundity, metabolic homeostasis, stress resistance and lifespan in worms, flies and mammals. Eight insulin-like peptides (DILP1-8) are found in Drosophila. Three of these (DILP2, 3 and 5) are produced by a set of median neurosecretory cells (insulin-producing cells, IPCs) in the brain. Activity in the IPCs of adult flies is regulated by glucose and several neurotransmitters and neuropeptides. One of these, short neuropeptide F (sNPF), regulates food intake, growth and Dilp transcript levels in IPCs via the sNPF receptor (sNPFR1) expressed on IPCs. Here we identify a set of brain neurons that utilizes sNPF to activate the IPCs. These sNPF-expressing neurons (dorsal lateral peptidergic neurons, DLPs) also produce the neuropeptide corazonin (CRZ) and have axon terminations impinging on IPCs. Knockdown of either sNPF or CRZ in DLPs extends survival in flies exposed to starvation and alters carbohydrate and lipid metabolism. Expression of sNPF in DLPs in the sNPF mutant background is sufficient to rescue wild-type metabolism and response to starvation. Since CRZ receptor RNAi in IPCs affects starvation resistance and metabolism, similar to peptide knockdown in DLPs, it is likely that also CRZ targets the IPCs. Knockdown of sNPF, but not CRZ in DLPs decreases transcription of Dilp2 and 5 in the brain, suggesting different mechanisms of action on IPCs of the two co-released peptides. Our findings indicate that sNPF and CRZ co-released from a small set of neurons regulate IPCs, stress resistance and metabolism in adult Drosophila.
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In insects, CRZ signaling regulates aspects of metabolism.
“also in insects, CRZ signaling regulates both aspects of metabolism (40, 80)”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [80]
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Extending Healthy Life Span—From Yeast to Humans
Fontana, L., Partridge, L., & Longo, V. D. (2010) · Science · doi:10.1126/science.1172539 · Cited by 3,023 · see citing works
Using FlyAtlas to identify better Drosophila melanogaster models of human disease
Chintapalli, V. R., Wang, J., & Dow, J. A. T. (2007) · Nature Genetics · doi:10.1038/ng2049 · Cited by 1,479 · see citing works
CRF and CRF receptors: role in stress responsivity and other behaviors
Bale, T. L., & Vale, W. W. (2004) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.44.101802.121410 · Cited by 1,270 · see citing works
Brogiolo, W., Stocker, H., Ikeya, T., Rintelen, F., Fernandez, R., & Hafen, E. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00068-9 · Cited by 1,255 · see citing works
Ablation of Insulin-Producing Neurons in Flies: Growth and Diabetic Phenotypes
Rulifson, E. J., Kim, S. K., & Nusse, R. (2002) · Science · doi:10.1126/science.1070058 · Cited by 1,064 · see citing works
Tools for neuroanatomy and neurogenetics in Drosophila
Pfeiffer, B. D., Jenett, A., Hammonds, A. S., Ngo, T.-T. B., Misra, S., Murphy, C., Scully, A. L., Carlson, J. W., Wan, K. H., Laverty, T., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0803697105 · Cited by 974 · see citing works
Broughton, S. J., Piper, M. D. W., Ikeya, T., Bass, T. M., Jacobson, J., Driege, Y., Martinez, P., Hafen, E., Withers, D. J., Leevers, S. J., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405775102 · Cited by 822 · see citing works
A nutrient sensor mechanism controls Drosophila growth
Colombani, J., Raisin, S., Pantalacci, S., Radimerski, T., Montagne, J., & Léopold, P. (2003) · Cell · doi:10.1016/s0092-8674(03)00713-x · Cited by 809 · see citing works
Ikeya, T., Galic, M., Belawat, P., Nairz, K., & Hafen, E. (2002) · Current Biology · doi:10.1016/s0960-9822(02)01043-6 · Cited by 765 · see citing works
Molecular Evolution and Functional Characterization of Drosophila Insulin-Like Peptides
Grönke, S., Clarke, D.-F., Broughton, S. J., Andrews, T. D., & Partridge, L. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000857 · Cited by 651 · see citing works
Lee, G., & Park, J. H. (2004) · Genetics · doi:10.1534/genetics.167.1.311 · Cited by 621 · see citing works
Remote Control of Insulin Secretion by Fat Cells in Drosophila
Géminard, C., Rulifson, E. J., & Léopold, P. (2009) · Cell Metabolism · doi:10.1016/j.cmet.2009.08.002 · Cited by 585 · see citing works
Neuropeptides and Neuropeptide Receptors in the Drosophila melanogaster GenomePreprint
Hewes, R. S., & Taghert, P. H. (2001) · Genome Research · doi:10.1101/gr.169901 · Cited by 541 · see citing works
Secreted Peptide Dilp8 Coordinates Drosophila Tissue Growth with Developmental Timing
Colombani, J., Andersen, D. S., & Léopold, P. (2012) · Science · doi:10.1126/science.1216689 · Cited by 525 · see citing works
Imaginal Discs Secrete Insulin-Like Peptide 8 to Mediate Plasticity of Growth and Maturation
Garelli, A., Gontijo, A. M., Miguela, V., Caparrós, E., & Domínguez, M. (2012) · Science · doi:10.1126/science.1216735 · Cited by 504 · see citing works
Diabetic Larvae and Obese Flies—Emerging Studies of Metabolism in Drosophila
Baker, K. D., & Thummel, C. S. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.09.002 · Cited by 467 · see citing works
Electrical Silencing of Drosophila Pacemaker Neurons Stops the Free-Running Circadian Clock
Nitabach, M. N., Blau, J., & Holmes, T. C. (2002) · Cell · doi:10.1016/s0092-8674(02)00737-7 · Cited by 452 · see citing works
ATP-sensitive K+ channels and insulin secretion: their role in health and disease
Ashcroft, F., & Gribble, F. (1999) · Diabetologia · doi:10.1007/s001250051247 · Cited by 435 · see citing works
Park, Y., Kim, Y., & Adams, M. E. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.162276199 · Cited by 388 · see citing works
Molecular mechanisms of metabolic regulation by insulin in Drosophila
Teleman, A. A. (2009) · Biochemical Journal · doi:10.1042/bj20091181 · Cited by 387 · see citing works
Drosophila Short Neuropeptide F Regulates Food Intake and Body Size
Lee, K., You, K., Choo, J., Han, Y., & Yu, K. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m407842200 · Cited by 348 · see citing works
Regulatory peptides in fruit fly midgut
Veenstra, J. A., Agricola, H., & Sellami, A. (2008) · Cell and Tissue Research · doi:10.1007/s00441-008-0708-3 · Cited by 325 · see citing works
Cognigni, P., Bailey, A. P., & Miguel‐Aliaga, I. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2010.12.010 · Cited by 325 · see citing works
Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster☆
Broeck, J. V. (2001) · Peptides · doi:10.1016/s0196-9781(00)00376-4 · Cited by 321 · see citing works
Nässel, D. R., & Wegener, C. (2011) · Peptides · doi:10.1016/j.peptides.2011.03.013 · Cited by 316 · see citing works
Self-citationMolecular identification of the insect adipokinetic hormone receptors
Staubli, F., Jørgensen, T. J. D., Cazzamali, G., Williamson, M. P., Lenz, C., Søndergaard, L., Roepstorff, P., & Grimmelikhuijzen, C. J. P. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052556499 · Cited by 302 · see citing works
Isolation and structure of corazonin, a cardioactive peptide from the American cockroach
Veenstra, J. A. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)80727-6 · Cited by 291 · see citing works
Role of insulin-like signalling in Drosophila lifespan
Giannakou, M. E., & PARTRIDGE, L. (2007) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.02.007 · Cited by 283 · see citing works
Candidate Gustatory Interneurons Modulating Feeding Behavior in the Drosophila Brain
Melcher, C., & Pankratz, M. J. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030305 · Cited by 278 · see citing works
Identification of a Neural Circuit that Underlies the Effects of Octopamine on Sleep:Wake Behavior
Crocker, A. J., Shahidullah, M., Levitan, I. B., & Sehgal, A. (2010) · Neuron · doi:10.1016/j.neuron.2010.01.032 · Cited by 275 · see citing works
Tawfik, A. I., Tanaka, S., LOOF, A. D., et al. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.12.7083 · Cited by 270 · see citing works
A novel diuretic hormone receptor inDrosophila: evidence for conservation of CGRP signaling
Johnson, E. C., Shafer, O. T., Trigg, J. S., Park, J. H., Schooley, D. A., Dow, J. A. T., & Taghert, P. H. (2005) · Journal of Experimental Biology · doi:10.1242/jeb.01529 · Cited by 254 · see citing works
Bharucha, K. N., Tarr, P., & Zipursky, S. L. (2008) · Journal of Experimental Biology · doi:10.1242/jeb.016451 · Cited by 239 · see citing works
Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling
Lee, K., Kwon, O., Lee, J. H., Kwon, K., Min, K., Jung, S., Kim, A., You, K., Tatar, M., & Yu, K. (2008) · Nature Cell Biology · doi:10.1038/ncb1710 · Cited by 230 · see citing works
Allatostatin-A neurons inhibit feeding behavior in adult Drosophila
Hergarden, A. C., Tayler, T. D., & Anderson, D. J. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1200778109 · Cited by 214 · see citing works
Corazonin receptor signaling in ecdysis initiation
Kim, Y., Spalovská, I., Cho, K.-H., Zitnanova, I., Park, Y., Adams, M. E., & Žitňan, D. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0305291101 · Cited by 206 · see citing works
Broughton, S. J., Alic, N., Slack, C., Bass, T. M., Ikeya, T., Vinti, G., Tommasi, A. M., Driege, Y., Hafen, E., & Partridge, L. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003721 · Cited by 194 · see citing works
Fernández‐Ayala, D. J. M., Sanz, A., Vartiainen, S., Kemppainen, K. K., Babušiak, M., Mustalahti, E., Costa, R., Tuomela, T., Zeviani, M., Chung, J., et al. (2009) · Cell Metabolism · doi:10.1016/j.cmet.2009.03.004 · Cited by 181 · see citing works
Nässel, D. R., Enell, L. E., Santos, J. G., Wegener, C., & Johard, H. A. D. (2008) · BMC Neuroscience · doi:10.1186/1471-2202-9-90 · Cited by 179 · see citing works
Self-citationLocalization of an insulin-like peptide in brains of two flies
Cao, C., & Brown, M. R. (2001) · Cell and Tissue Research · doi:10.1007/s004410100367 · Cited by 172 · see citing works
TheDhgene ofDrosophila melanogasterencodes a diuretic peptide that acts through cyclic AMP
Cabrero, P., Radford, J. C., Broderick, K. E., Costes, L., Veenstra, J. A., Spana, E. P., Davies, S. A., & Dow, J. A. T. (2002) · Journal of Experimental Biology · doi:10.1242/jeb.205.24.3799 · Cited by 163 · see citing works
Characterization of the short neuropeptide F receptor from Drosophila melanogaster
Mertens, I., Meeusen, T., Huybrechts, R., LOOF, A. D., & Schoofs, L. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02351-3 · Cited by 142 · see citing works
Broughton, S. J., Slack, C., Alic, N., Metaxakis, A., Bass, T. M., Driege, Y., & Partridge, L. (2010) · Aging Cell · doi:10.1111/j.1474-9726.2010.00558.x · Cited by 124 · see citing works
Johard, H. A. D., Enell, L. E., Gustafsson, E., Trifilieff, P., Veenstra, J. A., & Nässel, D. R. (2008) · Journal of Comparative Neurology · doi:10.1002/cne.21636 · Cited by 120 · see citing works
Self-citationLuo, J., Becnel, J., Nichols, C. D., & Nässel, D. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0789-0 · Cited by 114 · see citing works
Self-citationBirse, R. T., Söderberg, J. A. E., Luo, J., et al. (2011) · Journal of Experimental Biology · doi:10.1242/jeb.062091 · Cited by 112 · see citing works
Self-citationMolecular cloning and functional expression of a corazonin receptor
Cazzamali, G., Saxild, N. P. E., & Grimmelikhuijzen, C. J. P. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02398-7 · Cited by 111 · see citing works
Neuroanatomical studies of period gene expression in the hawkmoth, Manduca sexta
Wise, S. K., Davis, N. T., Tyndale, E., Noveral, J., Folwell, M. G., Bedian, V., Emery, I. F., & Siwicki, K. K. (2002) · The Journal of Comparative Neurology · doi:10.1002/cne.10242 · Cited by 109 · see citing works
Zhao, Y., Bretz, C. A., Hawksworth, S. A., Hirsh, J., & Johnson, E. C. (2010) · PLoS ONE · doi:10.1371/journal.pone.0009141 · Cited by 104 · see citing works
Kaplan, D. D., Zimmermann, G., Suyama, K. L., Meyer, T., & Scott, M. P. (2008) · Genes & Development · doi:10.1101/gad.1670508 · Cited by 104 · see citing works
Control of Metabolism and Growth Through Insulin-Like Peptides inDrosophila
Géminard, C., Arquier, N., Layalle, S., Bourouis, M., Slaidina, M., Delanoue, R., Bjordal, M., Ohanna, M., Ma, M., Colombani, J., et al. (2006) · Diabetes · doi:10.2337/db06-s001 · Cited by 102 · see citing works
Enell, L. E., Kapan, N., Söderberg, J. A. E., Kahsai, L., & Nässel, D. R. (2010) · PLoS ONE · doi:10.1371/journal.pone.0015780 · Cited by 101 · see citing works
Self-citationFunctional characterization of a neuropeptide F‐like receptor from Drosophila melanogaster
Feng, G., Reale, V., Chatwin, H. M., Kennedy, K., Venard, R., Ericsson, C., Yu, K., Evans, P. D., & Hall, L. M. (2003) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.2003.02719.x · Cited by 101 · see citing works
Antonova, Y., Arik, A. J., Moore, W., Riehle, M. A., & Brown, M. R. (2011) · Elsevier eBooks · doi:10.1016/b978-0-12-384749-2.10002-0 · Cited by 101 · see citing works
Yoon, J. G., & Stay, B. (1995) · The Journal of Comparative Neurology · doi:10.1002/cne.903630310 · Cited by 100 · see citing works
Does corazonin signal nutritional stress in insects?
Veenstra, J. A. (2009) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2009.09.008 · Cited by 99 · see citing works
Isolation and Structure of the Drosophila Corazonin Gene
Veenstra, J. A. (1994) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1994.2458 · Cited by 96 · see citing works
Boerjan, B., Verleyen, P., Huybrechts, J., Schoofs, L., & LOOF, A. D. (2009) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2009.09.004 · Cited by 92 · see citing works
Choi, Y. J., Lee, G., Hall, J. C., & Park, J. H. (2005) · The Journal of Comparative Neurology · doi:10.1002/cne.20419 · Cited by 86 · see citing works
Predel, R., Neupert, S., Russell, W. K., Scheibner, O., & Nachman, R. J. (2006) · Peptides · doi:10.1016/j.peptides.2006.10.011 · Cited by 83 · see citing works
Li, X. F., Bowe, J. E., Lightman, S. L., & O’Byrne, K. T. (2004) · Endocrinology · doi:10.1210/en.2004-0950 · Cited by 77 · see citing works
Fridell, Y.-W. C., Hoh, M., Kréneisz, O., Hosier, S., Chang, C.-Y., Scantling, D. R., Mulkey, D. K., & Helfand, S. L. (2009) · Aging · doi:10.18632/aging.100067 · Cited by 75 · see citing works
Glucose increases activity and Ca2+ in insulin-producing cells of adult Drosophila
Kréneisz, O., Chen, X., Fridell, Y.-W. C., & Mulkey, D. K. (2010) · Neuroreport · doi:10.1097/wnr.0b013e3283409200 · Cited by 74 · see citing works
JNK signaling in insulin‐producing cells is required for adaptive responses to stress in Drosophila
Karpac, J., Hull‐Thompson, J., Falleur, M., & Jasper, H. (2009) · Aging Cell · doi:10.1111/j.1474-9726.2009.00476.x · Cited by 74 · see citing works
Lee, G., Kim, K., Kikuno, K., Wang, Z., Choi, Y., & Park, J. H. (2007) · Cell and Tissue Research · doi:10.1007/s00441-007-0549-5 · Cited by 69 · see citing works
Kahsai, L., Carlsson, M. A., Winther, Å. M. E., & Nässel, D. R. (2012) · Neuroscience · doi:10.1016/j.neuroscience.2012.02.007 · Cited by 65 · see citing works
Self-citationCantera, R., Veenstra, J. A., & Nässel, D. R. (1994) · The Journal of Comparative Neurology · doi:10.1002/cne.903500405 · Cited by 59 · see citing works
Self-citationLi, X. F., Bowe, J. E., Mitchell, J. C., Brain, S. D., Lightman, S. L., & O’Byrne, K. T. (2004) · Endocrinology · doi:10.1210/en.2003-1609 · Cited by 57 · see citing works
Yew, J. Y., Wang, Y., Barteneva, N. S., Dikler, S., Kutz‐Naber, K. K., Li, L., & Kravitz, E. A. (2009) · Journal of Proteome Research · doi:10.1021/pr800601x · Cited by 55 · see citing works
Lee, K., Hong, S.-H., Kim, A., et al. (2009) · FEBS Letters · doi:10.1016/j.febslet.2009.07.024 · Cited by 39 · see citing works
Bergland, A. O., Chae, H.-S., Kim, Y.-J., & Tatar, M. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1002631 · Cited by 35 · see citing works
Anopheles gambiae corazonin: gene structure, expression and effect on mosquito heart physiology
Hillyer, J. F., Estévez‐Lao, T. Y., Funkhouser-Jones, L. J., & Aluoch, V. A. (2012) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2012.01140.x · Cited by 32 · see citing works
Choi, S., Lee, G., Monahan, P., et al. (2008) · The Journal of Comparative Neurology · doi:10.1002/cne.21594 · Cited by 30 · see citing works
Haamedi, S. N., Karten, H. J., & Djamgoz, M. B. A. (2001) · The Journal of Comparative Neurology · doi:10.1002/1096-9861(20010319)431:4 · Cited by 8 · see citing works
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- Authors
- Kapan, N., Lushchak, O. V., Luo, J., & Nässel, D. R.
- Affiliations
- Stockholm University (SE)
- Corresponding author
- Nässel, Dick R.
- Venue
- Cellular and Molecular Life Sciences
- Published
- 2012-07-25
- Volume
- 69
- Issue
- 23
- Pages
- 4051-4066
- ISSN
- 1420-682X (ISSN-L)
- 1420-9071
- PMC
- PMC11114645
- OpenAlex
- W1982288484
- Cited by
- 169
- References
- 74
- Citations by year
- 2026: 13 · 2025: 20 · 2024: 24 · 2023: 7 · 2022: 8 · 2021: 15 · 2020: 10 · 2019: 17 · 2018: 5 · 2017: 7 · 2016: 11 · 2015: 8 · 2014: 9 · 2013: 14 · 2012: 1
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cellular and Molecular Life Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- short neuropeptide F, corazonin, insulin-producing cells, Drosophila brain, starvation resistance, stress response, carbohydrate metabolism, lipid metabolism, DILP2, neuropeptide receptors
- MeSH terms
- Animals, Brain, Carbohydrates, Drosophila melanogaster, Hemolymph, Insulin, Lipids, Neurons, Neuropeptides, Neurosecretory Systems, Stress, Physiological, Receptors, Neuropeptide, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Drosophila Proteins, Animals, Genetically Modified, RNA Interference, Green Fluorescent Proteins, Insulins
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