The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
The Fanconi Anemia Pathway Protects Genome Integrity from R-loops
Open accessAbstract
Co-transcriptional RNA-DNA hybrids (R loops) cause genome instability. To prevent harmful R loop accumulation, cells have evolved specific eukaryotic factors, one being the BRCA2 double-strand break repair protein. As BRCA2 also protects stalled replication forks and is the FANCD1 member of the Fanconi Anemia (FA) pathway, we investigated the FA role in R loop-dependent genome instability. Using human and murine cells defective in FANCD2 or FANCA and primary bone marrow cells from FANCD2 deficient mice, we show that the FA pathway removes R loops, and that many DNA breaks accumulated in FA cells are R loop-dependent. Importantly, FANCD2 foci in untreated and MMC-treated cells are largely R loop dependent, suggesting that the FA functions at R loop-containing sites. We conclude that co-transcriptional R loops and R loop-mediated DNA damage greatly contribute to genome instability and that one major function of the FA pathway is to protect cells from R loops.
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Cites
DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis
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Interaction of the Fanconi Anemia Proteins and BRCA1 in a Common Pathway
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Biallelic Inactivation of BRCA2 in Fanconi Anemia
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R Loops: From Transcription Byproducts to Threats to Genome Stability
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Self-citationR-Loop Formation Is a Distinctive Characteristic of Unmethylated Human CpG Island Promoters
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R loops: new modulators of genome dynamics and function
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Self-citationInactivation of the SR Protein Splicing Factor ASF/SF2 Results in Genomic Instability
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Cited by
R-Loops as Cellular Regulators and Genomic Threats
Crossley, M. P., Bocek, M., & Cimprich, K. A. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.01.024 · Cited by 746
The Fanconi anaemia pathway: new players and new functions
Ceccaldi, R., Sarangi, P., & D'Andrea, A. D. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.48 · Cited by 720
Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells
Garaycoechea, J. I., Crossan, G. P., Langevin, F., Mulderrig, L., Louzada, S., Yang, F., Guilbaud, G., Park, N., Roerink, S., Nik-Zainal, S., et al. (2018) · Nature · doi:10.1038/nature25154 · Cited by 389
Hereditary breast and ovarian cancer: new genes in confined pathways
Nielsen, F. C., van Overeem Hansen, T., & Sørensen, C. S. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2016.72 · Cited by 367
Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1
Nguyen, H. D., Yadav, T., Giri, S., Saez, B., Graubert, T. A., & Zou, L. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.01.029 · Cited by 267
Metadata
- Authors
- García-Rubio, M. L., Pérez-Calero, C., Barroso, S. I., Tumini, E., Herrera-Moyano, E., Rosado, I. V., & Aguilera, A.
- Venue
- PLOS Genetics
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-11-19
- Volume
- 11
- Issue
- 11
- Pages
- e1005674
- ISSN
- 1553-7390 (ISSN-L)
- 1553-7404
- OpenAlex
- W2207325914
- Cited by
- 304
- References
- 45
- FWCI
- 11.32
- Type
- journal article
- Language
- en
- Open access
- gold
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- García-Rubio, M. L., Pérez-Calero, C., Barroso, S. I., Tumini, E., Herrera-Moyano, E., Rosado, I. V., & Aguilera, A. (2015). The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. PLOS Genetics. https://doi.org/10.1371/journal.pgen.1005674
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