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Cited by 12Phagocytosis and Immune RegulationCell death mechanisms and regulationInvertebrate Immune Response Mechanisms

bfc, a novel serpent co-factor for the expression of croquemort, regulates efferocytosis in Drosophila melanogaster

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Zheng, Qian, Gao, Ning, Sun, Qiling, Li, Xiaowen, Wang, Yanzhe, Xiao, Hui·2021·PLOS Genetics
DOI 10.1371/journal.pgen.1009947PMC PMC8673676
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Abstract

Efferocytosis is the process by which phagocytes recognize, engulf, and digest (or clear) apoptotic cells during development. Impaired efferocytosis is associated with developmental defects and autoimmune diseases. In Drosophila melanogaster, recognition of apoptotic cells requires phagocyte surface receptors, including the scavenger receptor CD36-related protein, Croquemort (Crq, encoded by crq). In fact, Crq expression is upregulated in the presence of apoptotic cells, as well as in response to excessive apoptosis. Here, we identified a novel gene bfc(booster for croquemort), which plays a role in efferocytosis, specifically the regulation of the crq expression. We found that Bfc protein interacts with the zinc finger domain of the GATA transcription factor Serpent (Srp), to enhance its direct binding to the crq promoter; thus, they function together in regulating crq expression and efferocytosis. Overall, we show that Bfc serves as a Srp co-factor to upregulate the transcription of the crq encoded receptor, and consequently boosts macrophage efferocytosis in response to excessive apoptosis. Therefore, this study clarifies how phagocytes integrate apoptotic cell signals to mediate efferocytosis.

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Authors
Zheng, Q., Gao, N., Sun, Q., Li, X., Wang, Y., & Xiao, H.
Affiliations
Shaanxi Normal University (CN)
Corresponding author
Xiao, Hui
ORCID
Venue
PLOS Genetics
Publisher
Public Library of Science (PLoS)
Published
2021-12-03
Volume
17
Issue
12
Pages
e1009947
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1009947
PMC
PMC8673676
OpenAlex
W3216813228
Cited by
12
References
1
Citations by year
2026: 3 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 2
FWCI
0.56
Type
journal article
Language
en
Update notices
new_version (2021-12-15)
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation Key Project (breeding program) of China (91954114)
  • National Natural Science Foundation of China (31671439)
  • National Natural Science Foundation of China Youth Program (31801164)
  • the program of Innovative Research Team for the Central Universities of Shaanxi Normal University (GK202001004)
  • the Fundamental Research Key Project Funds for the Central Universities of Shaanxi Normal University (GK202007009)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
Keywords
efferocytosis, serpents, GATA transcription factors, transcriptional cofactors, macrophages, Drosophila melanogaster
MeSH terms
Animals, Drosophila melanogaster, Macrophages, Phagocytes, Phagocytosis, Receptors, Cell Surface, Apoptosis, Gene Expression Regulation, Developmental, CD36 Antigens, Drosophila Proteins, GATA Transcription Factors, Receptors, Scavenger

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