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Cited by 117Rabies epidemiology and controlRNA Interference and Gene DeliveryVirology and Viral Diseases

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

A thermostable messenger RNA based vaccine against rabies

Open access
Stitz, Lothar, Vogel, Annette, Schnee, Margit, Voss, Daniel, Rauch, Susanne, Mutzke, Thorsten, Ketterer, Thomas, Kramps, Thomas, Petsch, Benjamin·2017·PLOS Neglected Tropical Diseases
DOI 10.1371/journal.pntd.0006108PMC PMC5737050
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Abstract

Although effective rabies virus vaccines have been existing for decades, each year, rabies virus infections still cause around 50.000 fatalities worldwide.Most of these cases occur in developing countries, where these vaccines are not available.The reasons for this are the prohibitive high costs of cell culture or egg grown rabies virus vaccines and the lack of a functional cold chain in many regions in which rabies virus is endemic.Here, we describe the excellent temperature resistance of a non-replicating mRNA based rabies virus vaccine encoding the rabies virus glycoprotein (RABV-G).Prolonged storage of the vaccine from -80˚C to up to +70˚C for several months did not impact the protective capacity of the mRNA vaccine.Efficacy after storage was demonstrated by the induction of rabies specific virus neutralizing antibodies and protection in mice against lethal rabies infection.Moreover, storing the vaccine at oscillating temperatures between +4˚and +56˚C for 20 cycles in order to simulate interruptions of the cold chain during vaccine transport, did not affect the vaccine's immunogenicity and protective characteristics, indicating that maintenance of a cold chain is not essential for this vaccine. Author summaryConventional prophylactic vaccines require transport and storage under controlled temperatures in an unbroken cold chain.Therefore, distribution of many vaccines is restricted to areas where the cold chain can be maintained which excludes especially rural areas in many countries from continues vaccine supply.Unfortunately, some diseases that can be prevented by vaccination, like the rabies virus infection, are still endemic in such areas.Therefore, logistic reasons often prevent delivery of life saving vaccines to areas in which they are most needed.Here, we describe an mRNA vaccine encoding the rabies virus glycoprotein (RABV-G) that remains protective in a mouse challenge model upon storage at highly variable temperatures.These results suggest that such a vaccine allows storage outside the cold chain and can therefore reach all areas of the world where rabies

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  1. Montagnon B, Fanget B (1996) Purified Vero cell vaccine for humans, WHO Laboratory techniques in rabies 4th edition, chapter 26. 4.

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Metadata

Authors
Stitz, L., Vogel, A., Schnee, M., Voss, D., Rauch, S., Mutzke, T., Ketterer, T., Kramps, T., & Petsch, B.
Affiliations
  • Friedrich-Loeffler-Institut (DE)
  • CureVac (Germany) (DE)
Corresponding author
Petsch, Benjamin
ORCID
Venue
PLOS Neglected Tropical Diseases
Publisher
Public Library of Science (PLoS)
Published
2017-12-07
Volume
11
Issue
12
Pages
e0006108
ISSN
  • 1935-2727 (ISSN-L)
  • 1935-2735
DOI
10.1371/journal.pntd.0006108
PMC
PMC5737050
OpenAlex
W2772901255
Cited by
117
References
18
Citations by year
2026: 5 · 2025: 9 · 2024: 12 · 2023: 19 · 2022: 26 · 2021: 22 · 2020: 14 · 2019: 5 · 2018: 4
FWCI
3.42
Type
journal article
Language
en
Update notices
new_version (2017-12-19)
Open access
Licence
Locations
  • PLoS neglected tropical diseases (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • OpenAgrar (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Bundesministerium für Bildung und Forschung (02PK2178)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Rabies epidemiology and control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
rabies virus, mRNA vaccines, rabies virus glycoprotein, thermostable vaccine, cold chain, vaccine storage, vaccine transport, virus-neutralizing antibodies, non-replicating vaccine, rabies prevention
MeSH terms
Immunogenicity, Vaccine, Animals, Antibodies, Viral, Antigens, Viral, Glycoproteins, Rabies, Rabies Vaccines, Rabies virus, RNA, Messenger, Temperature, Vaccines, Synthetic, Viral Envelope Proteins, Mice, Antibodies, Neutralizing, Vaccine Potency

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