The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Transdermal Influenza Immunization with Vaccine-Coated Microneedle Arrays
Open accessAbstract
BACKGROUND: Influenza is a contagious disease caused by a pathogenic virus, with outbreaks all over the world and thousands of hospitalizations and deaths every year. Due to virus antigenic drift and short-lived immune responses, annual vaccination is required. However, vaccine coverage is incomplete, and improvement in immunization is needed. The objective of this study is to investigate a novel method for transdermal delivery using metal microneedle arrays (MN) coated with inactivated influenza virus to determine whether this route is a simpler and safer approach than the conventional immunization, capable to induce robust immune responses and confer protection against lethal virus challenge. METHODOLOGY/PRINCIPAL FINDINGS: Inactivated A/Aichi/2/68 (H3N2) influenza virus was coated on metal microneedle arrays and applied to mice as a vaccine in the caudal dorsal skin area. Substantial antibody titers with hemagglutination inhibition activity were detected in sera collected two and four weeks after a single vaccine dose. Challenge studies in mice with 5 x LD(50) of mouse adapted Aichi virus demonstrated complete protection. Microneedle vaccination induced a broad spectrum of immune responses including CD4+ and CD8+ responses in the spleen and draining lymph node, a high frequency of antigen-secreting cells in the lung and induction of virus-specific memory B-cells. In addition, the use of MN showed a dose-sparing effect and a strong Th2 bias when compared to an intramuscular (IM) reference immunization. CONCLUSIONS/SIGNIFICANCE: The present results show that delivery of inactivated influenza virus through the skin using metal microneedle arrays induced strong humoral and cellular immune responses capable of conferring protection against virus challenge as efficiently as intramuscular immunization, which is the standard vaccination route. In view of the convenience of delivery and the potential for self-administration, vaccine-coated metal microneedles may provide a novel and highly effective immunization method.
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Cites
Influenza-Associated Hospitalizations in the United States
Thompson, W. W. (2004) · JAMA · doi:10.1001/jama.292.11.1333 · Cited by 2,213
Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells
Stevens, T. L., Bossie, A., Sanders, V. M., Fernandez-Botran, R., Coffman, R. L., Mosmann, T. R., & Vitetta, E. S. (1988) · Nature · doi:10.1038/334255a0 · Cited by 933 · cited 1×
“Elevated IFN-c production after IM vaccination is consistent with the heightened Th1-like response indicated by the reduced IgG1/IgG2a ratio in the IM groups, since IFN-c production is associated with IgG2a production [37].”
BackgroundHolland, D., Booy, R., Looze, F. D., Eizenberg, P., McDonald, J., Karrasch, J., McKeirnan, M., Salem, H., Mills, G., Reid, J., et al. (2008) · The Journal of Infectious Diseases · doi:10.1086/590434 · Cited by 254
Cited by
Microneedles for drug and vaccine delivery
Kim, Y.-C., Park, J.-H., & Prausnitz, M. R. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.04.005 · Cited by 1,760
Self-citationLarrañeta, E., Lutton, R. E. M., Woolfson, A. D., & Donnelly, R. F. (2016) · Materials Science and Engineering: R: Reports · doi:10.1016/j.mser.2016.03.001 · Cited by 964
Dissolving polymer microneedle patches for influenza vaccination
Sullivan, S. P., Koutsonanos, D. G., del Pilar Martin, M., Lee, J. W., Zarnitsyn, V., Choi, S.-O., Murthy, N., Compans, R. W., Skountzou, I., & Prausnitz, M. R. (2010) · Nature Medicine · doi:10.1038/nm.2182 · Cited by 948
Self-citationYang, C., Skiena, S., Futcher, B., Mueller, S., & Wimmer, E. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1307473110 · Cited by 103
Metadata
- Authors
- Koutsonanos, D. G., Martin, M. D. P., Zarnitsyn, V. G., Sullivan, S. P., Compans, R. W., Prausnitz, M. R., & Skountzou, I.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-03-10
- Volume
- 4
- Issue
- 3
- Pages
- e4773
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2090299109
- Cited by
- 198
- References
- 3
- FWCI
- 14.24
- Type
- journal article
- Language
- en
- Open access
- gold
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