The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways
Open accessAbstract
High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular polymerase extension cloning (CPEC). This method uses a single polymerase to assemble and clone multiple inserts with any vector in a one-step reaction in vitro. No restriction digestion, ligation, or single-stranded homologous recombination is required. In this study, we elucidate the CPEC reaction mechanism and demonstrate its usage in demanding synthetic biology applications such as one-step assembly and cloning of complex combinatorial libraries and multi-component pathways.
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Cites
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Self-citation
Cited by
Methods for the directed evolution of proteins
Packer, M. S., & Liu, D. R. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3927 · Cited by 991
Large-scale de novo DNA synthesis: technologies and applications
Kosuri, S., & Church, G. M. (2014) · Nature Methods · doi:10.1038/nmeth.2918 · Cited by 869
Directed Evolution: Methodologies and Applications
Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00260 · Cited by 719
Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
Paddon, C. J., & Keasling, J. D. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3240 · Cited by 675
Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology
Hughes, R. A., & Ellington, A. D. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023812 · Cited by 411
Metadata
- Authors
- Quan, J., & Tian, J.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-07-30
- Volume
- 4
- Issue
- 7
- Pages
- e6441
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2022186026
- Cited by
- 517
- References
- 20
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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