Student or educator? Sign up with your school email and get 6 months of Pro free.
Cited by 953

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Li, Mamie Z, Elledge, Stephen J·2007·Nature Methods

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/nmeth1010

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 22

  1. Smith, H.O. & Wilcox, K.W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51, 379–391 (1970). · DOI · Paper page
  2. Danna, K. & Nathans, D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc. Natl. Acad. Sci. USA 68, 2913–2917 (1971). · DOI · Paper page
  3. Cohen, S.N., Chang, A.C., Boyer, H.W. & Helling, R.B. Construction of biologically functional bacterial plasmids in vitro. Proc. Natl. Acad. Sci. USA 70, 3240–3244 (1973). · DOI · Paper page
  4. Backman, K. & Ptashne, M. Maximizing gene expression on a plasmid using recombination in vitro. Cell 13, 65–71 (1978). · DOI
  5. Hutchison, C.A. et al. Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem. 253, 6551–6560 (1978). · DOI · Paper page
  6. Rumsby, G. An introduction to PCR techniques. PCR Methods Mol. Biol. 324, 75–89 (2006).
  7. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. & Pease, L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51–59 (1989). · DOI · Paper page
  8. Liu, Q., Li, M.Z., Liebham, D., Cortez, D. & Elledge, S.J. The univector plasmid fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8, 1300–1309 (1998). · DOI · Paper page
  9. Hartley, J.L., Temple, G.F. & Brasch, M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 10, 1788–1795 (2000). · DOI · Paper page
  10. Walhout, A.J. et al. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328, 575–592 (2000). · DOI · Paper page
  11. Bethke, B. & Sauer, B. Segmental genomic replacement by Cre-mediated recombination: Genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Res. 25, 2828–2834 (1997). · doi:10.1093/nar/25.14.2828
  12. Nebert, D.W., Dalton, T.P., Stuart, G.W. & Carvan, M.J. Gene-swap knock-in cassette in mice to study allelic differences in human genes. Ann. NY Acad. Sci. 919, 148–170 (2000). · DOI
  13. Siegel, R.W. et al. Recombinatorial cloning using heterologous lox sites. Genome Res. 14, 1119–1129 (2004). · DOI
  14. Li, M.Z. & Elledge, S.J. MAGIC: An in vivo genetic method for the rapid construction of recombinant DNA molecules. Nat. Genet. 37, 311–319 (2005). · DOI
  15. Amundsen, S.K. & Smith, G.R. Interchangeable parts of the Escherichia coli recombination machinery. Cell 112, 741–744 (2003). · DOI
  16. Kuzminov, A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63, 751–813 (1999). · DOI · Paper page
  17. Konforti, B.B. & Davis, R.W. 3′ homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli. Proc. Natl. Acad. Sci. USA 84, 690–694 (1987). · doi:10.1073/pnas.84.3.690
  18. Tillett, D. & Neilan, B.A. Enzyme-free cloning: A rapid method to clone PCR products independent of vector restriction enzyme sites. Nucleic Acids Res. 27, e26 (1999). · DOI
  19. Aslanidis, C. & de Jong, P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18, 6069–6074 (1990). · DOI · Paper page
  20. Haun, R.S., Servanti, I.M. & Moss, J. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. Biotechniques 13, 515–518 (1992).
  21. Aslanidis, C., de Jong, P.J. & Schmitz, G. Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products. PCR Methods Appl. 4, 172–177 (1994). · DOI
  22. Cheo, D.L. et al. Concerted assembly and cloning of multiple DNA fragments using in vitro site-specific recombination: Functional analysis of multi-site expression clones. Gen. Res. 14, 2111–2120 (2004). · DOI

Cited by

See all 953

Metadata

Authors
Li, M. Z., & Elledge, S. J.
Venue
Nature Methods
Published
2007-02-11
Volume
4
Issue
3
Pages
251-256
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2102440675
Cited by
953
References
1
FWCI
6.82
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. https://app.cochat.ai/papers/10.1038/nmeth1010
The paper
Li, M. Z., & Elledge, S. J. (2007). Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature Methods. https://doi.org/10.1038/nmeth1010

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.