The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Efficient Feeder-Free Episomal Reprogramming with Small Molecules
Open accessAbstract
Genetic reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) could offer replenishable cell sources for transplantation therapies. To fulfill their promises, human iPSCs will ideally be free of exogenous DNA (footprint-free), and be derived and cultured in chemically defined media free of feeder cells. Currently, methods are available to enable efficient derivation of footprint-free human iPSCs. However, each of these methods has its limitations. We have previously derived footprint-free human iPSCs by employing episomal vectors for transgene delivery, but the process was inefficient and required feeder cells. Here, we have greatly improved the episomal reprogramming efficiency using a cocktail containing MEK inhibitor PD0325901, GSK3β inhibitor CHIR99021, TGF-β/Activin/Nodal receptor inhibitor A-83-01, ROCK inhibitor HA-100 and human leukemia inhibitory factor. Moreover, we have successfully established a feeder-free reprogramming condition using chemically defined medium with bFGF and N2B27 supplements and chemically defined human ESC medium mTeSR1 for the derivation of footprint-free human iPSCs. These improvements enabled the routine derivation of footprint-free human iPSCs from skin fibroblasts, adipose tissue-derived cells and cord blood cells. This technology will likely be valuable for the production of clinical-grade human iPSCs.
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Cites
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Self-citationGeneration of germline-competent induced pluripotent stem cells
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Cited by
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280
Generation of induced pluripotent stem cells from urine
Zhou, T., Benda, C., Duzinger, S., Huang, Y., Li, X., Li, Y., Guo, X., Cao, G., Chen, S., Hao, L., et al. (2011) · Journal of the American Society of Nephrology · doi:10.1681/asn.2011010106 · Cited by 421
Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA
Warren, L., Ni, Y., Wang, J., & Guo, X. (2012) · Scientific Reports · doi:10.1038/srep00657 · Cited by 154
Matsa, E., Ahrens, J. H., & Wu, J. C. (2016) · Physiological Reviews · doi:10.1152/physrev.00036.2015 · Cited by 105
All Roads Lead to Induced Pluripotent Stem Cells: The Technologies of iPSC Generation
Hu, K. (2014) · Stem Cells and Development · doi:10.1089/scd.2013.0620 · Cited by 105
Metadata
- Authors
- Yu, J., Chau, K. F., Vodyanik, M. A., Jiang, J., & Jiang, Y.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-03-01
- Volume
- 6
- Issue
- 3
- Pages
- e17557
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2045952188
- Cited by
- 254
- References
- 35
- FWCI
- 10.35
- Type
- journal article
- Language
- en
- Open access
- gold
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