The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice
Open accessAbstract
When expression of more than one gene is required in cells, bicistronic or multicistronic expression vectors have been used. Among various strategies employed to construct bicistronic or multicistronic vectors, an internal ribosomal entry site (IRES) has been widely used. Due to the large size and difference in expression levels between genes before and after IRES, however, a new strategy was required to replace IRES. A self-cleaving 2A peptide could be a good candidate to replace IRES because of its small size and high cleavage efficiency between genes upstream and downstream of the 2A peptide. Despite the advantages of the 2A peptides, its use is not widespread because (i) there are no publicly available cloning vectors harboring a 2A peptide gene and (ii) comprehensive comparison of cleavage efficiency among various 2A peptides reported to date has not been performed in different contexts. Here, we generated four expression plasmids each harboring different 2A peptides derived from the foot-and-mouth disease virus, equine rhinitis A virus, Thosea asigna virus and porcine teschovirus-1, respectively, and evaluated their cleavage efficiency in three commonly used human cell lines, zebrafish embryos and adult mice. Western blotting and confocal microscopic analyses revealed that among the four 2As, the one derived from porcine teschovirus-1 (P2A) has the highest cleavage efficiency in all the contexts examined. We anticipate that the 2A-harboring cloning vectors we generated and the highest efficiency of the P2A peptide we demonstrated would help biomedical researchers easily adopt the 2A technology when bicistronic or multicistronic expression is required.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Jin, Y.-H., Jeon, E.-J., Li, Q.-L., Lee, Y. H., Choi, J.-K., Kim, W.-J., Lee, K.-Y., & Bae, S.-C. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313120200 · Cited by 211
Self-citationA muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis
Renfer, E., Amon-Hassenzahl, A., Steinmetz, P. R. H., & Technau, U. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909148107 · Cited by 164
Cited by
Long‐term expanding human airway organoids for disease modeling
Sachs, N., Papaspyropoulos, A., Zomer‐van Ommen, D. D., Heo, I., Böttinger, L., Klay, D., Weeber, F., Huelsz‐Prince, G., Iakobachvili, N., Amatngalim, G. D., et al. (2019) · The EMBO Journal · doi:10.15252/embj.2018100300 · Cited by 1,082
ATR prohibits replication catastrophe by preventing global exhaustion of RPA
Toledo, L. I., Altmeyer, M., Rask, M.-B., Lukas, C., Larsen, D. H., Povlsen, L. K., Bekker-Jensen, S., Mailand, N., Bartek, J., & Lukas, J. (2013) · Cell · doi:10.1016/j.cell.2013.10.043 · Cited by 911
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction
Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4172 · Cited by 944
High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells
Zhou, Y., Zhu, S., Cai, C., Yuan, P., Li, C., Huang, Y., & Wei, W. (2014) · Nature · doi:10.1038/nature13166 · Cited by 733
Heckl, D., Kowalczyk, M. S., Yudovich, D., Belizaire, R., Puram, R. V., McConkey, M. E., Thielke, A., Aster, J. C., Regev, A., & Ebert, B. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2951 · Cited by 555
Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hsp90 traps and stabilizes an unfolded kinase
Verba, K. A., Wang, R. Y.-R., Arakawa, A., Liu, Y., Shirouzu, M., Yokoyama, S., & Agard, D. A. (2016) · Science · doi:10.1126/science.aaf5023 · Cited by 424
Trans-presentation of IL-6 by dendritic cells is required for the priming of pathogenic TH17 cells
Heink, S., Yogev, N., Garbers, C., Herwerth, M., Aly, L., Gasperi, C., Husterer, V., Croxford, A. L., Möller-Hackbarth, K., Bartsch, H. S., et al. (2017) · Nature Immunology · doi:10.1038/ni.3632 · Cited by 386
HIV-1 uncoats in the nucleus near sites of integration
Burdick, R. C., Li, C., Munshi, M., Rawson, J. M. O., Nagashima, K., Hu, W.-S., & Pathak, V. K. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1920631117 · Cited by 313
Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region
Schlepckow, K., Monroe, K. M., Kleinberger, G., Cantuti‐Castelvetri, L., Parhizkar, S., Xia, D., Willem, M., Werner, G., Pettkus, N., Brunner, B., et al. (2020) · EMBO Molecular Medicine · doi:10.15252/emmm.201911227 · Cited by 324
Self-citationDesign and testing of a humanized porcine donor for xenotransplantation
Anand, R. P., Layer, J. V., Heja, D., Hirose, T., Lassiter, G., Firl, D. J., Paragas, V. B., Akkad, A., Chhangawala, S., Colvin, R. B., et al. (2023) · Nature · doi:10.1038/s41586-023-06594-4 · Cited by 302
Al‐Moyed, H., Cepeda, A. P., Jung, S., Moser, T., Kügler, S., & Reisinger, E. (2019) · EMBO Molecular Medicine · doi:10.15252/emmm.201809396 · Cited by 230
Pflueger, C., Tan, D., Swain, T., Nguyen, T., Pflueger, J., Nefzger, C., Polo, J. M., Ford, E., & Lister, R. (2018) · Genome Research · doi:10.1101/gr.233049.117 · Cited by 188
A DNMT3A mutation common in AML exhibits dominant-negative effects in murine ES cells
Kim, S. J., Zhao, H., Hardikar, S., Singh, A. K., Goodell, M. A., & Chen, T. (2013) · Blood · doi:10.1182/blood-2013-02-483487 · Cited by 180
TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis
Ikmi, A., McKinney, S. A., Delventhal, K. M., & Gibson, M. C. (2014) · Nature Communications · doi:10.1038/ncomms6486 · Cited by 165
Structure of a CLC chloride ion channel by cryo-electron microscopy
Park, E., Campbell, E. B., & MacKinnon, R. (2017) · Nature · doi:10.1038/nature20812 · Cited by 164
Universal recording of immune cell interactions in vivo
Nakandakari-Higa, S., Walker, S., Canesso, M. C. C., van der Heide, V., Chudnovskiy, A., Kim, D.-Y., Jacobsen, J. T., Parsa, R., Bilanovic, J., Parigi, S. M., et al. (2024) · Nature · doi:10.1038/s41586-024-07134-4 · Cited by 136
Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis
Watanabe, S., Mamer, L. E., Raychaudhuri, S., Luvsanjav, D., Eisen, J., Trimbuch, T., Söhl-Kielczynski, B., Fenske, P., Milosevic, I., Rosenmund, C., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.06.005 · Cited by 131
Davenport, E. C., Szulc, B. R., Drew, J., Taylor, J., Morgan, T., Higgs, N. F., López-Doménech, G., & Kittler, J. T. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.01.092 · Cited by 125
Pharmacological inhibition of the transcription factor PU.1 in leukemia
Antony-Debré, I., Paul, A., Leite, J., Mitchell, K., Kim, H. M., Carvajal, L. A., Todorova, T. I., Huang, K., Kumar, A., Farahat, A. A., et al. (2017) · Journal of Clinical Investigation · doi:10.1172/jci92504 · Cited by 120
Xu, J., Camacho, M., Xu, Y., Esser, V., Liu, X., Trimbuch, T., Pan, Y.-Z., Ma, C., Tomchick, D. R., Rosenmund, C., et al. (2017) · eLife · doi:10.7554/elife.22567 · Cited by 119
Lukoseviciute, M., Gavriouchkina, D., Williams, R. M., Hochgreb-Hagele, T., Senanayake, U., Chong-Morrison, V., Thongjuea, S., Repapi, E., Mead, A., & Sauka-Spengler, T. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.11.009 · Cited by 109
Buck, T., & Wijnholds, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124197 · Cited by 92
“Miscellaneous Size Description References 2A 75 Self-cleaving linker [159]”
BackgroundpEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences
Grier, A. E., Burleigh, S., Sahni, J., Clough, C. A., Cardot, V., Choe, D. C., Krutein, M. C., Rawlings, D. J., Jensen, M. C., Scharenberg, A. M., et al. (2016) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2016.21 · Cited by 95
Maricque, B. B., Chaudhari, H. G., & Cohen, B. A. (2019) · Nature Biotechnology · doi:10.1038/nbt.4285 · Cited by 83
D'hondt, S., Guillemyn, B., Syx, D., Symoens, S., De Rycke, R., Vanhoutte, L., Toussaint, W., Lambrecht, B. N., De Paepe, A., Keene, D. R., et al. (2018) · Matrix Biology · doi:10.1016/j.matbio.2018.03.008 · Cited by 79
ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes
Miki, K., Deguchi, K., Nakanishi-Koakutsu, M., Lucena-Cacace, A., Kondo, S., Fujiwara, Y., Hatani, T., Sasaki, M., Naka, Y., Okubo, C., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23816-3 · Cited by 76
Vierra, N. C., Dadi, P. K., Jeong, I., Dickerson, M., Powell, D. R., & Jacobson, D. A. (2015) · Diabetes · doi:10.2337/db15-0280 · Cited by 71
Cherry, C., Andorko, J. I., Krishnan, K., Mejías, J. C., Nguyen, H. H., Stivers, K. B., Gray-Gaillard, E. F., Ruta, A., Han, J., Hamada, N., et al. (2023) · GeroScience · doi:10.1007/s11357-023-00785-7 · Cited by 66
Joglekar, A. V., Hollis, R. P., Kuftinec, G., Senadheera, S., Chan, R., & Kohn, D. B. (2013) · Molecular Therapy · doi:10.1038/mt.2013.106 · Cited by 65
Sequential replication-coupled destruction at G1/S ensures genome stability
Coleman, K. E., Grant, G. D., Haggerty, R. A., Brantley, K., Shibata, E., Workman, B. D., Dutta, A., Varma, D., Purvis, J. E., & Cook, J. G. (2015) · Genes & Development · doi:10.1101/gad.263731.115 · Cited by 51
Oligodendrocyte calcium signaling promotes actin-dependent myelin sheath extension
Iyer, M., Kantarci, H., Cooper, M. H., Ambiel, N., Novak, S. W., Andrade, L. R., Lam, M., Jones, G., Münch, A. E., Yu, X., et al. (2024) · Nature Communications · doi:10.1038/s41467-023-44238-3 · Cited by 49
Xu, Y., Ma, S., Huang, Y., Chen, F., Chen, L., Ding, D., Zheng, Y., Li, H., Xiao, J., Feng, J., et al. (2019) · Vaccine · doi:10.1016/j.vaccine.2019.03.031 · Cited by 34
Dual-AAV delivery of large gene sequences to the inner ear
Reisinger, E. (2020) · Hearing Research · doi:10.1016/j.heares.2019.107857 · Cited by 37
Genetically encoded live-cell sensor for tyrosinated microtubules
Kesarwani, S., Lama, P., Chandra, A., Reddy, P. P., Jijumon, A. S., Bodakuntla, S., Rao, B. M., Janke, C., Das, R., & Sirajuddin, M. (2020) · Journal of Cell Biology · doi:10.1083/jcb.201912107 · Cited by 37
Toulmé, E., Salazar Lázaro, A., Trimbuch, T., Rizo, J., & Rosenmund, C. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2409636121 · Cited by 27
Rapid, biochemical tagging of cellular activity history in vivo
Zhang, R., Anguiano, M., Aarrestad, I. K., Lin, S., Chandra, J., Vadde, S. S., Olson, D. E., & Kim, C. K. (2024) · Nature Methods · doi:10.1038/s41592-024-02375-7 · Cited by 25
Cold shock domain-containing protein E1 is a posttranscriptional regulator of the LDL receptor
Smith, G. A., Padmanabhan, A., Lau, B. H., Pampana, A., Li, L., Lee, C. Y., Pelonero, A., Nishino, T., Sadagopan, N., Xia, V. Q., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abj8670 · Cited by 23
Self-citationDevelopment of Lentiviral Vectors for HIV-1 Gene Therapy with Vif-Resistant APOBEC3G
Delviks-Frankenberry, K. A., Ackerman, D., Timberlake, N. D., Hamscher, M., Nikolaitchik, O. A., Hu, W.-S., Torbett, B. E., & Pathak, V. K. (2019) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2019.10.024 · Cited by 21
Chorba, J. S., Galvan, A. M., & Shokat, K. M. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra117.000754 · Cited by 18
Neurexin‐β mediates the synaptogenic activity of amyloid precursor protein
Cvetkovska, V., Ge, Y., Xu, Q., Li, S., Zhang, P., & Craig, A. M. (2022) · The Journal of Neuroscience · doi:10.1523/jneurosci.0511-21.2022 · Cited by 17
Dzhivhuho, G., Holsey, J., Honeycutt, E., O’Farrell, H., Rekosh, D., Hammarskjold, M.-L., & Jackson, P. E. H. (2022) · Scientific Reports · doi:10.1038/s41598-022-21714-2 · Cited by 10
Visualizing WNT signaling in mammalian systems
van der Wal, T., & van Amerongen, R. (2023) · Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.02.001 · Cited by 2
Metadata
- Authors
- Kim, J. H., Lee, S.-R., Li, L.-H., Park, H.-J., Park, J.-H., Lee, K. Y., Kim, M.-K., Shin, B. A., & Choi, S.-Y.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-04-29
- Volume
- 6
- Issue
- 4
- Pages
- e18556
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2023545713
- Cited by
- 1,338
- References
- 2
- FWCI
- 9.64
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
