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Cited by 37Microtubule and mitosis dynamicsAdvanced Fluorescence Microscopy TechniquesCancer Treatment and Pharmacology

Genetically encoded live-cell sensor for tyrosinated microtubules

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Kesarwani, Shubham, Lama, Prakash, Chandra, Anchal, Reddy, P. Purushotam, Jijumon, A.S., Bodakuntla, Satish, Rao, Balaji M., Janke, Carsten +2 more·2020·Journal of Cell Biology
DOI 10.1083/jcb.201912107PMC PMC7659708

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Abstract

Microtubule cytoskeleton exists in various biochemical forms in different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known to affect microtubule stability, dynamics, and interaction with MAPs and motors in a specific manner, widely known as tubulin code hypothesis. At present, there exists no tool that can specifically mark tubulin PTMs in living cells, thus severely limiting our understanding of their dynamics and cellular functions. Using a yeast display library, we identified a binder against terminal tyrosine of α-tubulin, a unique PTM site. Extensive characterization validates the robustness and nonperturbing nature of our binder as tyrosination sensor, a live-cell tubulin nanobody specific towards tyrosinated microtubules. Using this sensor, we followed nocodazole-, colchicine-, and vincristine-induced depolymerization events of tyrosinated microtubules in real time and found each distinctly perturbs the microtubule polymer. Together, our work describes a novel tyrosination sensor and its potential applications to study the dynamics of microtubule and their PTM processes in living cells.

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  • Dynamic microtubules can be readily visualized in live cells using plus-end markers or a sensor for tyrosinated tubulin, whereas stable microtubules cannot currently be visualized live except upon cold treatment, microtubule-depolymerizing drugs, or immunocytochemistry for posttranslational modifications.

    “Dynamic MTs can be readily visualized in live cells using plus-end markers or the recently developed sensor for tyrosinated tubulin (Kesarwani et al., 2020); however, this is not the case for stable MTs, which can currently only be visualized upon cold treatment or exposure to MT-depolymerizing drugs, or approximated using immunocytochemistry for different PTMs.”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Kesarwani et al., 2020

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Authors
Kesarwani, S., Lama, P., Chandra, A., Reddy, P. P., Jijumon, A. S., Bodakuntla, S., Rao, B. M., Janke, C., Das, R., & Sirajuddin, M.
Affiliations
  • Manipal Academy of Higher Education (IN)
  • Tata Institute of Fundamental Research (IN)
  • Université Paris-Sud (FR)
  • North Carolina State University (US)
  • Institute for Stem Cell Biology and Regenerative Medicine (IN)
Corresponding author
Sirajuddin, Minhajuddin
ORCID
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2020-09-04
Volume
219
Issue
10
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.201912107
PMC
PMC7659708
OpenAlex
W3083369693
Cited by
37
References
4
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1.85
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journal article
Language
en
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  • The Journal of Cell Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research Explorer (The University of Manchester) (publishedVersion)
  • HAL (submittedVersion, other-oa)
Funders
  • inStem Graduate Program
  • Wellcome Trust DBT India Alliance (IA/E/15/1/502339)
  • Institut Curie
  • Agence Nationale de la Recherche (ANR-17-CE13-0021)
  • Institut National du Cancer (2014-PL BIO-11-ICR-1)
  • Fondation pour la Recherche Medicale (DEQ20170336756, FDT201805005465, FDT201904008210)
  • Horizon 2020 research and innovation program (675737)
  • DBT (BT/PR5081/INF/156/2012)
  • Tata Institute of Fundamental Research
  • DBT-Ramalingaswamy (BT/HRD/23/02/2006)
  • DBT/Wellcome Trust India Alliance (IA/I/14/2/501533)
  • European Molecular Biology Organization
  • CEFIPRA (5703-1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
Keywords
tubulin post-translational modifications, nanobodies, microtubule depolymerization, nocodazole, colchicine, vincristine, microtubule dynamics
MeSH terms
Colchicine, Cytoskeleton, Humans, Microtubule-Associated Proteins, Microtubules, Protein Processing, Post-Translational, Tubulin, Tyrosine, Vincristine, Nocodazole, HEK293 Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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