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Cited by 131Cellular transport and secretionNeuroscience and Neuropharmacology ResearchCaveolin-1 and cellular processes

Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis

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Watanabe, Shigeki, Mamer, Lauren Elizabeth, Raychaudhuri, Sumana, Luvsanjav, Delgermaa, Eisen, Julia, Trimbuch, Thorsten, Söhl-Kielczynski, Berit, Fenske, Pascal +3 more·2018·Neuron
DOI 10.1016/j.neuron.2018.06.005PMC PMC6086574
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Abstract

Ultrafast endocytosis generates vesicles from the plasma membrane as quickly as 50 ms in hippocampal neurons following synaptic vesicle fusion. The molecular mechanism underlying the rapid maturation of these endocytic pits is not known. Here we demonstrate that synaptojanin-1, and its partner endophilin-A, function in ultrafast endocytosis. In the absence of synaptojanin or endophilin, the membrane is rapidly invaginated, but pits do not become constricted at the base. The 5-phosphatase activity of synaptojanin is involved in formation of the neck, but 4-phosphatase is not required. Nevertheless, these pits are eventually cleaved into vesicles; within a 30-s interval, synaptic endosomes form and are resolved by clathrin-mediated budding. Then synaptojanin and endophilin function at a second step to aid with the removal of clathrin coats from the regenerated vesicles. These data together suggest that synaptojanin and endophilin can mediate membrane remodeling on a millisecond timescale during ultrafast endocytosis.

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  • Endocytic proteins such as Synaptojanin 1, Endophilin A, Syndapin 1, and Dynamin 1 can further curve the membrane and generate vesicles.

    “Thereafter, endocytic proteins such as Synaptojanin 1, Endophilin A, Syndapin 1, and Dynamin 1 can further curve the membrane and generate vesicles47–50.”

    From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [50]

    The Discussion section, “Model for endophilin and synaptojanin in vesicle cleavage” (page unavailable), proposes that endophilin and synaptojanin narrow the membrane neck so dynamin can cleave the vesicle. The cited paper does not identify Syndapin 1 as part of this process, and its relevant passages refer to dynamin without specifying Dynamin 1. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Authors
Watanabe, S., Mamer, L. E., Raychaudhuri, S., Luvsanjav, D., Eisen, J., Trimbuch, T., Söhl-Kielczynski, B., Fenske, P., Milosevic, I., Rosenmund, C., & Jorgensen, E. M.
Affiliations
  • Johns Hopkins University (US)
  • NeuroCure Cluster of Excellence (DE)
  • Columbia University (US)
Corresponding author
  • Watanabe, Shigeki
  • Rosenmund, Christian
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
2018-06
Volume
98
Issue
6
Pages
1184-1197.e6
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/j.neuron.2018.06.005
PMC
PMC6086574
OpenAlex
W2809799315
Cited by
131
References
6
Citations by year
2026: 10 · 2025: 19 · 2024: 19 · 2023: 11 · 2022: 20 · 2021: 13 · 2020: 15 · 2019: 17 · 2018: 6
FWCI
7.06
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Tzagournis Medical Research Endowment Fund
  • EMBO (ASTF 443-2012)
  • Grass Foundation
  • Johns Hopkins University
  • University of Chicago – MBL Lillie
  • National Science Foundation (1727271)
  • NIH (NS034307)
  • European Research Council (249939 SYNVGLUT)
  • German Research Council (Neurocure EXC 257, SFB 665, SFB 958, SFB889, Emmy Noether)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
Keywords
SYNJ1, synaptic vesicle endocytosis, membrane remodeling, hippocampal neurons, membrane invagination
MeSH terms
Acyltransferases, Animals, Cell Membrane, Clathrin, Endocytosis, Nerve Tissue Proteins, Neurons, Phosphoric Monoester Hydrolases, Endosomes, Synapses, Synaptic Vesicles, Mice, Knockout, Transport Vesicles, Clathrin-Coated Vesicles, Adaptor Proteins, Signal Transducing, Mice

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