The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
MicroRNA-145 Regulates Chondrogenic Differentiation of Mesenchymal Stem Cells by Targeting Sox9
Open accessAbstract
Chondrogenic differentiation of mesenchymal stem cells (MSCs) is accurately regulated by essential transcription factors and signaling cascades. However, the precise mechanisms involved in this process still remain to be defined. MicroRNAs (miRNAs) regulate various biological processes by binding target mRNA to attenuate protein synthesis. To investigate the mechanisms for miRNAs-mediated regulation of chondrogenic differentiation, we identified that miR-145 was decreased during transforming growth factor beta 3 (TGF-β3)-induced chondrogenic differentiation of murine MSCs. Subsequently, dual-luciferase reporter gene assay data demonstrated that miR-145 targets a putative binding site in the 3'-UTR of SRY-related high mobility group-Box gene 9 (Sox9) gene, the key transcription factor for chondrogenesis. In addition, over-expression of miR-145 decreased expression of Sox9 only at protein levels and miR-145 inhibition significantly elevated Sox9 protein levels. Furthermore, over-expression of miR-145 decreased mRNA levels for three chondrogenic marker genes, type II collagen (Col2a1), aggrecan (Agc1), cartilage oligomeric matrix protein (COMP), type IX collagen (Col9a2) and type XI collagen (Col11a1) in C3H10T1/2 cells induced by TGF-β3, whereas anti-miR-145 inhibitor increased the expression of these chondrogenic marker genes. Thus, our studies demonstrated that miR-145 is a key negative regulator of chondrogenic differentiation by directly targeting Sox9 at early stage of chondrogenic differentiation.
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Prediction of Mammalian MicroRNA Targets
Lewis, B. P., Shih, I.-H., Jones-Rhoades, M. W., Bartel, D. P., & Burge, C. B. (2003) · Cell · doi:10.1016/s0092-8674(03)01018-3 · Cited by 5,199
Combinatorial microRNA target predictions
Krek, A., Grün, D., Poy, M. N., Wolf, R., Rosenberg, L., Epstein, E. J., MacMenamin, P., da Piedade, I., Gunsalus, K. C., Stoffel, M., et al. (2005) · Nature Genetics · doi:10.1038/ng1536 · Cited by 4,603
miR-145 and miR-143 regulate smooth muscle cell fate and plasticity
Cordes, K. R., Sheehy, N. T., White, M. P., Berry, E. C., Morton, S. U., Muth, A. N., Lee, T.-H., Miano, J. M., Ivey, K. N., & Srivastava, D. (2009) · Nature · doi:10.1038/nature08195 · Cited by 1,585
The cartilage specific microRNA‐140 targets histone deacetylase 4 in mouse cells
Tuddenham, L., Wheeler, G., Ntounia-Fousara, S., Waters, J., Hajihosseini, M. K., Clark, I., & Dalmay, T. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.06.080 · Cited by 422
Cited by
Key transcription factors in the differentiation of mesenchymal stem cells
Almalki, S. G., & Agrawal, D. K. (2016) · Differentiation · doi:10.1016/j.diff.2016.02.005 · Cited by 420
The Signaling Pathways Involved in Chondrocyte Differentiation and Hypertrophic Differentiation
Li, J., & Dong, S. (2016) · Stem Cells International · doi:10.1155/2016/2470351 · Cited by 170
Self-citationMiR-145 functions as a tumor suppressor by directly targeting histone deacetylase 2 in liver cancer
Noh, J. H., Chang, Y. G., Kim, M. G., Jung, K. H., Kim, J. K., Bae, H. J., Eun, J. W., Shen, Q., Kim, S.-J., Kwon, S. H., et al. (2013) · Cancer Letters · doi:10.1016/j.canlet.2013.03.003 · Cited by 102
Lü, M.-H., Hu, C.-J., Chen, L., Peng, X., Chen, J., Hu, J.-Y., Teng, M., & Liang, G.-P. (2013) · PLoS ONE · doi:10.1371/journal.pone.0068972 · Cited by 43
Self-citationThe emerging functions of the p53-miRNA network in stem cell biology
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Metadata
- Authors
- Yang, B., Guo, H., Zhang, Y., Chen, L., Ying, D., & Dong, S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-07-20
- Volume
- 6
- Issue
- 7
- Pages
- e21679
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2117840333
- Cited by
- 268
- References
- 4
- FWCI
- 18.74
- Type
- journal article
- Language
- en
- Open access
- gold
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