The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Nucleotide Discrimination with DNA Immobilized in the MspA Nanopore
Open accessAbstract
Nanopore sequencing has the potential to become a fast and low-cost DNA sequencing platform. An ionic current passing through a small pore would directly map the sequence of single stranded DNA (ssDNA) driven through the constriction. The pore protein, MspA, derived from Mycobacterium smegmatis, has a short and narrow channel constriction ideally suited for nanopore sequencing. To study MspA's ability to resolve nucleotides, we held ssDNA within the pore using a biotin-NeutrAvidin complex. We show that homopolymers of adenine, cytosine, thymine, and guanine in MspA exhibit much larger current differences than in α-hemolysin. Additionally, methylated cytosine is distinguishable from unmethylated cytosine. We establish that single nucleotide substitutions within homopolymer ssDNA can be detected when held in MspA's constriction. Using genomic single nucleotide polymorphisms, we demonstrate that single nucleotides within random DNA can be identified. Our results indicate that MspA has high signal-to-noise ratio and the single nucleotide sensitivity desired for nanopore sequencing devices.
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Cites
Genome-wide association study identifies novel breast cancer susceptibility loci
Easton, D. F., Pooley, K. A., Dunning, A. M., Pharoah, P. D. P., Thompson, D., Ballinger, D. G., Struewing, J. P., Morrison, J., Field, H., Luben, R., et al. (2007) · Nature · doi:10.1038/nature05887 · Cited by 2,319
Admixture mapping identifies 8q24 as a prostate cancer risk locus in African-American men
Freedman, M. L., Haiman, C. A., Patterson, N., McDonald, G. J., Tandon, A., Waliszewska, A., Penney, K., Steen, R. G., Ardlie, K., John, E. M., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605832103 · Cited by 612
What would you do if you could sequence everything?
Kahvejian, A., Quackenbush, J., & Thompson, J. F. (2008) · Nature Biotechnology · doi:10.1038/nbt1494 · Cited by 189
Cited by
Three decades of nanopore sequencing
Deamer, D., Akeson, M., & Branton, D. (2016) · Nature Biotechnology · doi:10.1038/nbt.3423 · Cited by 1,292
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
Manrao, E. A., Derrington, I. M., Laszlo, A. H., Langford, K. W., Hopper, M. K., Gillgren, N., Pavlenok, M., Niederweis, M., & Gundlach, J. H. (2012) · Nature Biotechnology · doi:10.1038/nbt.2171 · Cited by 965
Self-citationDecoding long nanopore sequencing reads of natural DNA
Laszlo, A. H., Derrington, I. M., Ross, B. C., Brinkerhoff, H., Adey, A., Nova, I. C., Craig, J. M., Langford, K. W., Samson, J. M., Daza, R., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.2950 · Cited by 443
Self-citationDetection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA
Laszlo, A. H., Derrington, I. M., Brinkerhoff, H., Langford, K. W., Nova, I. C., Samson, J. M., Bartlett, J. J., Pavlenok, M., & Gundlach, J. H. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1310240110 · Cited by 321
Self-citationSingle-molecule site-specific detection of protein phosphorylation with a nanopore
Rosen, C. B., Rodriguez-Larrea, D., & Bayley, H. (2014) · Nature Biotechnology · doi:10.1038/nbt.2799 · Cited by 270
Metadata
- Authors
- Manrao, E. A., Derrington, I. M., Pavlenok, M., Niederweis, M., & Gundlach, J. H.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-10-04
- Volume
- 6
- Issue
- 10
- Pages
- e25723
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2170183499
- Cited by
- 159
- References
- 3
- FWCI
- 6.38
- Type
- journal article
- Language
- en
- Open access
- gold
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