The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Improved Detection of Bifidobacteria with Optimised 16S rRNA-Gene Based Pyrosequencing
Open accessAbstract
The 16S rRNA gene is conserved across all bacteria and as such is routinely targeted in PCR surveys of bacterial diversity. PCR primer design aims to amplify as many different 16S rRNA gene sequences from as wide a range of organisms as possible, though there are no suitable 100% conserved regions of the gene, leading to bias. In the gastrointestinal tract, bifidobacteria are a key genus, but are often under-represented in 16S rRNA surveys of diversity. We have designed modified, 'bifidobacteria-optimised' universal primers, which we have demonstrated detection of bifidobacterial sequence present in DNA mixtures at 2% abundance, the lowest proportion tested. Optimisation did not compromise the detection of other organisms in infant faecal samples. Separate validation using fluorescence in situ hybridisation (FISH) shows that the proportions of bifidobacteria detected in faecal samples were in agreement with those obtained using 16S rRNA based pyrosequencing. For future studies looking at faecal microbiota, careful selection of primers will be key in order to ensure effective detection of bifidobacteria.
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STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT
Martin Bland, J., & Altman, D. (1986) · The Lancet · doi:10.1016/s0140-6736(86)90837-8 · Cited by 47,965
Development of the Human Infant Intestinal Microbiota
Palmer, C., Bik, E. M., DiGiulio, D. B., Relman, D. A., & Brown, P. O. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050177 · Cited by 2,877
Review article: bifidobacteria as probiotic agents – physiological effects and clinical benefits
PICARD, C., FIORAMONTI, J., FRANCOIS, A., ROBINSON, T., NEANT, F., & MATUCHANSKY, C. (2005) · Alimentary Pharmacology & Therapeutics · doi:10.1111/j.1365-2036.2005.02615.x · Cited by 618
Synbiotics prevent asthma-like symptoms in infants with atopic dermatitis
van der Aa, L. B., van Aalderen, W. M. C., Heymans, H. S. A., Henk Sillevis Smitt, J., Nauta, A. J., Knippels, L. M. J., Ben Amor, K., Sprikkelman, A. B., & the Synbad Study Group (2011) · Allergy · doi:10.1111/j.1398-9995.2010.02416.x · Cited by 172
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Practical innovations for high-throughput amplicon sequencing
Lundberg, D. S., Yourstone, S., Mieczkowski, P., Jones, C. D., & Dangl, J. L. (2013) · Nature Methods · doi:10.1038/nmeth.2634 · Cited by 1,009
Evidence that the endometrial microbiota has an effect on implantation success or failure
Moreno, I., Codoñer, F. M., Vilella, F., Valbuena, D., Martinez-Blanch, J. F., Jimenez-Almazán, J., Alonso, R., Alamá, P., Remohí, J., Pellicer, A., et al. (2016) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2016.09.075 · Cited by 868
The role of bacteria in the pathogenesis and progression of idiopathic pulmonary fibrosis
Molyneaux, P. L., Cox, M. J., Willis-Owen, S. A. G., Mallia, P., Russell, K. E., Russell, A.-M., Murphy, E., Johnston, S. L., Schwartz, D. A., Wells, A. U., et al. (2014) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201403-0541oc · Cited by 567
Self-citationPrebiotic inulin-type fructans induce specific changes in the human gut microbiota
Vandeputte, D., Falony, G., Vieira-Silva, S., Wang, J., Sailer, M., Theis, S., Verbeke, K., & Raes, J. (2017) · Gut · doi:10.1136/gutjnl-2016-313271 · Cited by 554
Metadata
- Authors
- Sim, K., Cox, M. J., Wopereis, H., Martin, R., Knol, J., Li, M.-S., Cookson, W. O. C. M., Moffatt, M. F., & Kroll, J. S.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-03-28
- Volume
- 7
- Issue
- 3
- Pages
- e32543
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2084463234
- Cited by
- 199
- References
- 4
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- gold
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- Sim, K., Cox, M. J., Wopereis, H., Martin, R., Knol, J., Li, M.-S., Cookson, W. O. C. M., Moffatt, M. F., & Kroll, J. S. (2012). Improved Detection of Bifidobacteria with Optimised 16S rRNA-Gene Based Pyrosequencing. PLoS ONE. https://doi.org/10.1371/journal.pone.0032543
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
