The DOI matches its Crossref record; 66 of the 67 references it cites were found in a registry or an index.
Early Activation of Rat Skeletal Muscle IL-6/STAT1/STAT3 Dependent Gene Expression in Resistance Exercise Linked to Hypertrophy
Open accessAbstract
Cytokine interleukin-6 (IL-6) is an essential regulator of satellite cell-mediated hypertrophic muscle growth through the transcription factor signal transducer and activator of transcription 3 (STAT3). The importance of this pathway linked to the modulation of myogenic regulatory factors expression in rat skeletal muscle undergoing hypertrophy following resistance exercise, has not been investigated. In this study, the phosphorylation and nuclear localization of STAT3, together with IL-6/STAT3-responsive gene expression, were measured after both a single bout of resistance exercise and 10 weeks of training. Flexor Digitorum Profundus muscle samples from Wistar rats were obtained 2 and 6 hours after a single bout of resistance exercise and 72 h after the last bout of either 2, 4, or 10 weeks of resistance training. We observed an increase in IL-6 and SOCS3 mRNAs concomitant with phosphorylation of STAT1 and STAT3 after 2 and 6 hours of a single bout of exercise (p<0.05). STAT3-dependent early responsive genes such as CyclinD1 and cMyc were also upregulated whereas MyoD and Myf5 mRNAs were downregulated (p<0.05). BrdU-positive satellite cells increased at 2 and 6 hours after exercise (p<0.05). Muscle fiber hypertrophy reached up to 100% after 10 weeks of training and the mRNA expression of Myf5, c-Myc and Cyclin-D1 decreased, whereas IL-6 mRNA remained upregulated. We conclude that the IL-6/STAT1/STAT3 signaling pathway and its responsive genes after a single bout of resistance exercise are an important event regulating the SC pool and behavior involved in muscle hypertrophy after ten weeks of training in rat skeletal muscle.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“However, in a previous study that used rats of the same age and weight, we showed that the non-exercised control group had a mean FDP CSA value of 1440 ± 84 μm2 [14], which is notably lower than the value of 2291 ± 182 μm2 of the present trained control group.”
From Acute and chronic effects of Rhaponticum carthamoides and Rhodiola rosea extracts supplementation coupled to resistance exercise on muscle protein synthesis and mechanical power in rats (2020) · cites this paper as [14]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“We used a rat model of resistance exercise known to increase MPS [14].”
From Acute and chronic effects of Rhaponticum carthamoides and Rhodiola rosea extracts supplementation coupled to resistance exercise on muscle protein synthesis and mechanical power in rats (2020) · cites this paper as [14]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
1 retracted
Immunohistochemical detection of interleukin‐6 in human skeletal muscle fibers following exercise
Penkowa, M., Keller, C., Keller, P., Jauffred, S., & Pedersen, B. K. (2003) · The FASEB Journal · doi:10.1096/fj.03-0311fje · Cited by 157
RetractedA new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
SATELLITE CELL OF SKELETAL MUSCLE FIBERS
Mauro, A. (1961) · The Journal of Cell Biology · doi:10.1083/jcb.9.2.493 · Cited by 3,683
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
Pedersen, B. K., & Febbraio, M. A. (2008) · Physiological Reviews · doi:10.1152/physrev.90100.2007 · Cited by 2,388
The JAK/STAT signaling pathway
Rawlings, J. S., Rosler, K. M., & Harrison, D. A. (2004) · Journal of Cell Science · doi:10.1242/jcs.00963 · Cited by 1,858
Interleukin-6 in Biology and Medicine
Akira, S., Taga, T., & Kishimoto, T. (1993) · Advances in immunology · doi:10.1016/s0065-2776(08)60532-5 · Cited by 1,402
O’Shea, J. J., Gadina, M., & Schreiber, R. D. (2002) · Cell · doi:10.1016/s0092-8674(02)00701-8 · Cited by 1,137
IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans
Steensberg, A., Fischer, C. P., Keller, C., Møller, K., & Pedersen, B. K. (2003) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00074.2003 · Cited by 1,135
Mixed muscle protein synthesis and breakdown after resistance exercise in humans
Phillips, S. M., Tipton, K. D., Aarsland, A., Wolf, S. E., & Wolfe, R. R. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.1.e99 · Cited by 1,132
Campos, G., Luecke, T., Wendeln, H., Toma, K., Hagerman, F., Murray, T., Ragg, K., Ratamess, N., Kraemer, W., & Staron, R. (2002) · European Journal of Applied Physiology · doi:10.1007/s00421-002-0681-6 · Cited by 1,066
Flagged first, then by citations
1 of the 67 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Items 101–124 of 124
Redox signalling and microRNA feedback in exercise-mediated skeletal muscle remodelling
Qin, X., Goljanek‐Whysall, K., & McDonagh, B. (2025) · Advances in Redox Research · doi:10.1016/j.arres.2025.100133 · Cited by 1
Fovet, T., Issertine, M., Jacob, P., Ghislin, S., Laroche, N., Roffino, S., Camy, C., Theuil, J., Bertrand‐Gaday, C., Delobel, P., et al. (2025) · The FASEB Journal · doi:10.1096/fj.202501128r
Self-citationDDIT3 Governs Milk Production Traits by Targeting IL-6 to Induce Apoptosis in Dairy Cattle
Cui, X., Li, C., Wei, Z., Meng, H., Zhang, F., Liu, Y., Wu, C., & Yang, S. (2022) · Agriculture · doi:10.3390/agriculture13010117 · Cited by 1
de Souza, B. C. (2013) · Americanae (AECID Library) · Cited by 1
Training Load Responses Modelling in Elite Sports: How to Deal with Generalisation?Preprint
Imbach, F., Perrey, S., Chailan, R., Méline, T., & Candau, R. B. (2020) · Research Square · doi:10.21203/rs.3.rs-128940/v1 · Cited by 1
Self-citationExercise modulation of the alternative splicing landscape in human tissuesPreprint
Zhang, Z., Nudelman, G., Pinças, H., Iyer, G. R., Smith, G. R., Keshishian, H., Jin, C. A., Trappe, S. W., Katz, D. H., Burant, C., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.03.03.704460 · Cited by 1
The aging horse: Effects of inflammation on muscle satellite cells 1,2
Reed, S. A., LaVigne, E. K., Jones, A. K., Patterson, D. F., & Schauer, A. (2015)
Wei, J., Zhao, H., Jiang, Q., Wen, C., Huang, X., Ling, Y., & Ying, Y. (2021) · Research Square · doi:10.21203/rs.3.rs-768341/v1
Phillips, S. M. (2020) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.735320205.793571145
Cordingley, D. M., & Cornish, S. M. (2022) · OBM Geriatrics · doi:10.21926/obm.geriatr.2204206
Potential Therapeutic Strategies for Muscular Dystrophy
Gaina, G. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.102255
The Potential Benefits of Drug-Repositioning in Muscular Dystrophies
Lambrescu, I. M., Manole, E., Ceafalan, L. C., & Gaina, G. F. (2023) · IntechOpen eBooks · doi:10.5772/intechopen.110714
PERAN INTERLEUKIN-6 PADA OLAHRAGA : KAJIAN PUSTAKA
Nasution, S. D., Rahmi, R., & Fauziyah, Y. (2024) · Jurnal Medika Malahayati · doi:10.33024/jmm.v7i4.11460
Ma, Z., Li, P., Zhu, Y., Fan, L., Xu, G., Wu, R., Zhu, C., Wang, L., Wang, S., Gao, P., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4795887
Karisa, P., Sylviana, N., Rosdianto, A. M., Goenawan, H., & Setiawan, I. (2025) · Research Square · doi:10.21203/rs.3.rs-6464671/v1 · Cited by 1
Abe, T., Isomura, E. T., Kuwahara, T., Mitsui, R., Matsukawa, M., Nakagawa, K., Tanaka, S., & Tabata, Y. (2025) · Regenerative Therapy · doi:10.1016/j.reth.2025.05.016
Roumanille, R., Vernus, B., Brioche, T., Descossy, V., Ba, C. T. V., Campredon, S., Philippe, A. G., Delobel, P., Bertrand‐Gaday, C., Chopard, A., et al. (2020) · Figshare · doi:10.6084/m9.figshare.13245385
Self-citationNikzad, M. B., Sardar, M. A., Aghababaeian, A., Azimi, S. A., Shafiee, M., & Abbasian, S. (2025) · International Journal of Cancer Management · doi:10.5812/ijcm-163669
Fard, M. B., Choobineh, S., Soori, R., & Mazaheri, Z. (2026) · Experimental Physiology · doi:10.1113/ep093390
Abuhassan, Q., Al‐Ameer, H. J., Jyothi, S. R., Nayak, P. P., Pandurangan, P., Mukherjee, G., Sinha, A., & Hazratkulova, O. (2026) · Immunology · doi:10.1111/imm.70170
The Health Benefits of Exercise: Molecular and Cellular Mechanisms
Ying, P., Wang, H., Lu, X., Zhang, W., Lin, Z., Wang, K., Li, S., Chen, L., Jiang, M., Hamad, S., et al. (2026) · MedComm · doi:10.1002/mco2.70926 · Cited by 1
Yang, Z., Wu, C., Zou, J., & Yuan, Y. (2026) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2026.101955
Karisa, P., Sylviana, N., Rosdianto, A. M., Goenawan, H., Syamsunarno, M. R. A. A., & Setiawan, I. (2026) · Cardiology Research and Practice · doi:10.1155/crp/2976039
Metadata
- Authors
- Begue, G., Douillard, A., Galbes, O., Rossano, B., Vernus, B., Candau, R., & Py, G.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Corresponding author
- Py, Guillaume
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-02-22
- Volume
- 8
- Issue
- 2
- Pages
- e57141
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3579782
- OpenAlex
- W2031180727
- Cited by
- 124
- References
- 67
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 4 · 2023: 6 · 2022: 15 · 2021: 8 · 2020: 14 · 2019: 11 · 2018: 8 · 2017: 16 · 2016: 11 · 2015: 9 · 2014: 4 · 2013: 2
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- World Anti-Doping Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Keywords
- interleukin-6, STAT3, STAT1, resistance training, skeletal muscle hypertrophy, satellite cells, SOCS3, myogenic regulatory factors, MyoD, Myf5, c-Myc, cyclin D1, flexor digitorum profundus, exercise-induced gene expression, muscle fiber hypertrophy
- MeSH terms
- Animals, Gene Expression Regulation, Male, Muscle Proteins, Physical Conditioning, Animal, RNA, Messenger, Interleukin-6, Polymerase Chain Reaction, Rats, Wistar, DNA Primers, Muscle, Skeletal, Cell Proliferation, STAT1 Transcription Factor, STAT3 Transcription Factor, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
