The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Microgravity Induces Pelvic Bone Loss through Osteoclastic Activity, Osteocytic Osteolysis, and Osteoblastic Cell Cycle Inhibition by CDKN1a/p21
Open accessAbstract
Bone is a dynamically remodeled tissue that requires gravity-mediated mechanical stimulation for maintenance of mineral content and structure. Homeostasis in bone occurs through a balance in the activities and signaling of osteoclasts, osteoblasts, and osteocytes, as well as proliferation and differentiation of their stem cell progenitors. Microgravity and unloading are known to cause osteoclast-mediated bone resorption; however, we hypothesize that osteocytic osteolysis, and cell cycle arrest during osteogenesis may also contribute to bone loss in space. To test this possibility, we exposed 16-week-old female C57BL/6J mice (n = 8) to microgravity for 15-days on the STS-131 space shuttle mission. Analysis of the pelvis by µCT shows decreases in bone volume fraction (BV/TV) of 6.29%, and bone thickness of 11.91%. TRAP-positive osteoclast-covered trabecular bone surfaces also increased in microgravity by 170% (p = 0.004), indicating osteoclastic bone degeneration. High-resolution X-ray nanoCT studies revealed signs of lacunar osteolysis, including increases in cross-sectional area (+17%, p = 0.022), perimeter (+14%, p = 0.008), and canalicular diameter (+6%, p = 0.037). Expression of matrix metalloproteinases (MMP) 1, 3, and 10 in bone, as measured by RT-qPCR, was also up-regulated in microgravity (+12.94, +2.98 and +16.85 fold respectively, p<0.01), with MMP10 localized to osteocytes, and consistent with induction of osteocytic osteolysis. Furthermore, expression of CDKN1a/p21 in bone increased 3.31 fold (p<0.01), and was localized to osteoblasts, possibly inhibiting the cell cycle during tissue regeneration as well as conferring apoptosis resistance to these cells. Finally the apoptosis inducer Trp53 was down-regulated by -1.54 fold (p<0.01), possibly associated with the quiescent survival-promoting function of CDKN1a/p21. In conclusion, our findings identify the pelvic and femoral region of the mouse skeleton as an active site of rapid bone loss in microgravity, and indicate that this loss is not limited to osteoclastic degradation. Therefore, this study offers new evidence for microgravity-induced osteocytic osteolysis, and CDKN1a/p21-mediated osteogenic cell cycle arrest.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source doesn’t support the claim as written.
In the citing paper
“They also documented osteocyte apoptosis as reflected by increases in lacunar and canalicular diameters [60].”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“They found that microgravity reduced the bone volume by 6.3% and bone thickness by 11.9% and increased the number of bone-surface TRAP-positive osteoclasts by 170% compared to that of ground controls, indicating enhanced osteoclast-mediated bone resorption.”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“conditions of microgravity and simulated microgravity decrease the mineral content and cortical and trabecular microstructures of bone”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“decrease osteoblastogenesis and osteoblast secretion of OPG”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“increase osteoclast differentiation, fusion, and expression of regulatory and osteoclast-specific genes”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Experimental — accuracy not yet measured
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“increase osteoclast secretion of sclerostin”
From Osteoclasts and Microgravity (2020) · cites this paper as [60]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Vogelstein, B. (1992) · Cell · doi:10.1016/0092-8674(92)90421-8 · Cited by 1,849
Cellular and molecular mechanisms of bone remodeling
Raggatt, L. J., & Partridge, N. C. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.r109.041087 · Cited by 1,402
Correlation of Terminal Cell Cycle Arrest of Skeletal Muscle with Induction of p21 by MyoD
Halevy, O., Novitch, B. G., Spicer, D. B., Skapek, S. X., Rhee, J., Hannon, G. J., Beach, D., & Lassar, A. B. (1995) · Science · doi:10.1126/science.7863327 · Cited by 1,139
Matrix metalloproteinases: a tail of a frog that became a prince
Brinckerhoff, C., & Matrisian, L. M. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm763 · Cited by 1,112
Vico, L., Collet, P., Guignandon, A., Lafage-Proust, M.-H., Thomas, T., Rehailia, M., & Alexandre, C. (2000) · The Lancet · doi:10.1016/s0140-6736(00)02217-0 · Cited by 821
Matrix metalloproteinases (MMPs) in health and disease: an overview
Malemud, C. (2006) · Frontiers in Bioscience · doi:10.2741/1915 · Cited by 705
The cell biology of bone metabolism
Datta, H. K., Ng, W. F., Walker, J. A., Tuck, S. P., & Varanasi, S. S. (2008) · Journal of Clinical Pathology · doi:10.1136/jcp.2007.048868 · Cited by 614
Yoshihara, Y., Nakamura, H., Obata, K., Yamada, H., Hayakawa, T., Fujikawa, K., & Okada, Y. (2000) · Annals of the Rheumatic Diseases · doi:10.1136/ard.59.6.455 · Cited by 566
Mechanosensation and transduction in osteocytes
Klein-Nulend, J., Bakker, A. D., Bacabac, R. G., Vatsa, A., & Weinbaum, S. (2013) · Bone · doi:10.1016/j.bone.2012.10.013 · Cited by 528
Ge, C., Xiao, G., Jiang, D., & Franceschi, R. T. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200610046 · Cited by 510
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–198 of 198
Reptiles in Space Missions: Results and Perspectives
Gulimova, V. I., Proshchina, A. E., Kharlamova, A. S., Krivova, Y. S., Barabanov, V., Berdiev, R. K., Асадчиков, В. Е., Buzmakov, A. V., Zolotov, D. A., & Saveliev, S. V. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20123019 · Cited by 20
Mechanobiological Implications of Cancer Progression in Space
Kim, H., Shin, Y. J., & Kim, D. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.740009 · Cited by 19
Kroge, S. V., Wölfel, E. M., Buravkova, L. B., Atiakshin, D., Markina, E. A., Schinke, T., Rolvien, T., Busse, B., & Jähn, K. (2021) · European Cells and Materials · doi:10.22203/ecm.v042a16 · Cited by 17
Microgravity Effects and Aging Physiology: Similar Changes or Common Mechanisms?
Ratushnyy, A. Y., & Buravkova, L. B. (2023) · Biochemistry (Moscow) · doi:10.1134/s0006297923110081 · Cited by 16
Harlow, L., Sahbani, K., Nyman, J. S., Cardozo, C. P., Bauman, W. A., & Tawfeek, H. A. W. (2017) · Physiological Reports · doi:10.14814/phy2.13446 · Cited by 19
No Signature of Osteocytic Osteolysis in Cortical Bone from Lactating NMRI Mice
Wittig, N. K., Birkbak, M. E., Bach-Gansmo, F. L., Pacureanu, A., Wendelboe, M. H., Brüel, A., Thomsen, J. S., & Birkedal, H. (2019) · Calcified Tissue International · doi:10.1007/s00223-019-00569-2 · Cited by 16
Osumi, R., Wang, Z., Ishihara, Y., Odagaki, N., Iimura, T., & Kamioka, H. (2020) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-020-01135-9 · Cited by 16
Zhu, M.-C., Jin, X., Wu, B., Nie, J., & Li, Y. H. (2014) · Genetics and Molecular Research · doi:10.4238/2014.august.7.20 · Cited by 16
Okuma, K. F., Menuki, K., Tsukamoto, M., Tajima, T., Fukuda, H., Okada, Y., Mori, T., Tsuchiya, T., Kawamoto, T., Yoshida, Y., et al. (2017) · Calcified Tissue International · doi:10.1007/s00223-017-0285-0 · Cited by 15
Forces associated with launch into space do not impact bone fracture healing
Childress, P., Brinker, A., Gong, C.-M. S., Harris, J. S., Olivos, D. J., Rytlewski, J. D., Scofield, D. C., Choi, S. Y., Shirazi‐Fard, Y., McKinley, T. O., et al. (2017) · Life Sciences in Space Research · doi:10.1016/j.lssr.2017.11.002 · Cited by 15
Giuliani, A., Mazzoni, S., Ruggiu, A., Canciani, B., Cancedda, R., & Tavella, S. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.00181 · Cited by 15
Counteracting Muscle Atrophy on Earth and in Space via Nanofluidics Delivery of Formoterol
Ballerini, A., Chua, C. Y. X., Rhudy, J. R., Šušnjar, A., Trani, N. D., Jain, P., Laue, G., Lubicka, D., Shirazi‐Fard, Y., Mauro Ferrari, B., et al. (2020) · Advanced Therapeutics · doi:10.1002/adtp.202000014 · Cited by 15
The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs
Xu, D., Guo, Y., Zhang, M., & Sun, Y. (2018) · Chinese Journal of Traumatology · doi:10.1016/j.cjtee.2018.04.004 · Cited by 15
Simulated microgravity modulates the mesenchymal stromal cell response to inflammatory stimulation
Ratushnyy, A. Y., Yakubets, D., Andreeva, E. R., & Buravkova, L. B. (2019) · Scientific Reports · doi:10.1038/s41598-019-45741-8 · Cited by 14
Liu, J., Wang, J., & Guo, Y. (2020) · Molecules · doi:10.3390/molecules25040782 · Cited by 14
Sahbani, K., Cardozo, C. P., Bauman, W. A., & Tawfeek, H. A. W. (2021) · Endocrinology · doi:10.1210/endocr/bqab230 · Cited by 15
The Skeletal Cellular and Molecular Underpinning of the Murine Hindlimb Unloading Model
Garg, P., Strigini, M., Peurière, L., Vico, L., & Iandolo, D. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.749464 · Cited by 19
Checa, S., Hesse, B., Roschger, P., Aido, M., Duda, G. N., Raum, K., & Willie, B. M. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.04.020 · Cited by 13
Stem cell research in space: Advancing regenerative medicine beyond Earth
Mozneb, M., Arzt, M., Moses, J., Escopete, S. S., Wiegand, L., & Sharma, A. (2025) · Cell stem cell · doi:10.1016/j.stem.2025.09.001 · Cited by 12
Juran, C. M., Žvirblytė, J., Cheng-Campbell, M., Blaber, E. A., & Almeida, E. A. C. (2021) · Stem Cell Research · doi:10.1016/j.scr.2021.102513 · Cited by 12
Self-citationCazzaniga, A., Castiglioni, S., & Maier, J. A. M. (2014) · BioMed Research International · doi:10.1155/2014/857934 · Cited by 12
Magnesium-containing implants enhance bone healing: A mechanobiological perspective
Wen, Z., Lei, L., Zhang, H., Jin, Z., Shan, Z., Liu, W., Tong, W., Xu, J., & Qin, L. (2025) · Mechanobiology in Medicine · doi:10.1016/j.mbm.2025.100161 · Cited by 15
Moonga, S. S., & Qin, Y. (2018) · Journal of Orthopaedic Translation · doi:10.1016/j.jot.2018.02.002 · Cited by 11
Genthial, R., Gerbaix, M., Farlay, D., Vico, L., Beaurepaire, E., Débarre, D., & Gourrier, A. (2019) · PLoS ONE · doi:10.1371/journal.pone.0209079 · Cited by 11
Liu, H., Guo, J., Li, Y., Zhang, Y.-S., Wang, J., Gao, J., Deng, Y., & Li, Y. (2020) · Molecules · doi:10.3390/molecules25194391 · Cited by 11
Markina, E. A., Andreeva, E. R., Andrianova, I. V., Сотнезова, Е. В., & Buravkova, L. B. (2018) · Stem Cells and Development · doi:10.1089/scd.2017.0264 · Cited by 11
Matrix metalloproteinases in extracellular matrix remodeling: molecular, cellular and tissue aspects
Шишкина, В. В., Антакова, Л. Н., Zolotareva, S. N., & Atyakshin, D. A. (2022) · Journal of Anatomy and Histopathology · doi:10.18499/2225-7357-2022-11-3-93-108 · Cited by 10
Sun, Q., Xu, L., Hu, Z., Liu, J., Yu, T., Li, M., Zhang, S., & Shi, F. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46090572 · Cited by 9
Knockdown of CD44 inhibits the alteration of osteoclast function induced by simulated microgravity
Li, Y., Gao, X., Ling, S., Zhong, G., Sun, W., Liu, C., Li, J., Song, J., Zhao, D., Jin, X., et al. (2019) · Acta Astronautica · doi:10.1016/j.actaastro.2019.01.032 · Cited by 9
Zhang, X., Zhang, L., Xu, L., Li, G., Wang, K., Xue, T., Sun, Q., Tang, H., Cao, X., Hu, Z., et al. (2022) · Journal of Personalized Medicine · doi:10.3390/jpm12122030 · Cited by 9
AOP Report: Development of an adverse outcome pathway for deposition of energy leading to bone loss
Sandhu, S., Keyworth, M., Karimi‐Jashni, S., Alomar, D., Smith, B. J., Kozbenko, T., Doty, S. B., Hocking, R., Hamada, N., Reynolds, R. J., et al. (2024) · Environmental and Molecular Mutagenesis · doi:10.1002/em.22631 · Cited by 11
Mozneb, M., Arzt, M., Mesci, P., Martin, D. M. N., Pohlman, S., Lawless, G. M., Doraisingam, S., Neyadi, S. A., Barnawi, R., Qarni, A. A., et al. (2024) · npj Microgravity · doi:10.1038/s41526-024-00435-y · Cited by 9
Glucocorticoid: A potential role in microgravity-induced bone loss
Yang, J., Yang, Z., Li, W., Xue, Y., Xu, H., Li, J., & Shang, P. (2017) · Acta Astronautica · doi:10.1016/j.actaastro.2017.08.007 · Cited by 9
Mimic microgravity effect on muscle transcriptome under ionizing radiation
Tran, K. N., & Choi, J. (2021) · Life Sciences in Space Research · doi:10.1016/j.lssr.2021.12.002 · Cited by 9
Osteocytes: Regulating the Mineral Reserves?
Arnett, T. R. (2013) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2119 · Cited by 9
DeGuire, J. R., Mak, I. L., Lavery, P., Agellon, S., Wykes, L., & Weiler, H. A. (2014) · Bone · doi:10.1016/j.bone.2014.12.005 · Cited by 9
Pintus, G., Akram, M. A., Giordo, R., Caggiari, G., & Majdalawieh, A. F. (2025) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2025.11.048 · Cited by 9
Oral Tissue Responses to Travel in Space
Mednieks, M. I., & Hand, A. R. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.86728 · Cited by 9
Huyan, T., Du, Y., Peng, H., Hu, X. L., Wu, C., Li, Q., Dong, D., Li, J., & Shang, P. (2019) · Acta Astronautica · doi:10.1016/j.actaastro.2019.04.045 · Cited by 9
Yu, K., Tuladhar, A., Dankberg, S., Dai, C., & McGee‐Lawrence, M. E. (2025) · Biomolecules · doi:10.3390/biom15111534 · Cited by 9
Krivonosov, Y. S., Gulimova, V. I., Buzmakov, A. V., Zolotov, D. A., Cedola, A., Bukreeva, I., Асадчиков, В. Е., & Saveliev, S. V. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.752893 · Cited by 7
Sonawane, R., Patil, S., Rahaman, J., & Mukherjee, D. (2025) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2025.152055 · Cited by 7
Strategies for studying bone loss in microgravity
Fournier, R., & Harrison, R. E. (2020) · REACH · doi:10.1016/j.reach.2020.100036 · Cited by 7
The Effects of Radiation and Hindlimb Unloading on Rat Bone Marrow Progenitor Cells
Markina, E. A., Kokhan, V. S., Roe, M. P., Andrianova, I. V., Shtemberg, A. S., & Buravkova, L. B. (2018) · Cell and Tissue Biology · doi:10.1134/s1990519x18030069 · Cited by 7
Cahill, R., Blaber, E. A., Juran, C. M., Cheng-Campbell, M., Alwood, J. S., Shirazi‐Fard, Y., & Almeida, E. A. C. (2025) · PLoS ONE · doi:10.1371/journal.pone.0317307 · Cited by 6
Self-citationTyrina, E. A., Yakubets, D., Markina, E. A., & Buravkova, L. B. (2024) · Cells · doi:10.3390/cells13221921 · Cited by 6
Guo, Y., Waisberg, E., Ong, J., Kumar, R., & Lee, A. G. (2024) · Acta Astronautica · doi:10.1016/j.actaastro.2024.12.033 · Cited by 7
Stem Cell Biology and Tissue Engineering in Space
Arzt, M., Jenkins, A., & Sharma, A. (2022) · Wiley eBooks · doi:10.1002/9783527830909.ch5 · Cited by 6
Peurière, L., Mastrandrea, C. J., Vanden‐Bossche, A., Linossier, M., Thomas, M., Normand, M., Lafage‐Proust, M., & Vico, L. (2022) · SSRN Electronic Journal · doi:10.2139/ssrn.4232740 · Cited by 6
Out of this World: Wound Healing on Earth and in Space
Balukoff, N. C., Houk, G., Gonzalez, T., Berton, Y., Ronfard, V., Pastar, I., & Tomic‐Canic, M. (2025) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2024.12.024 · Cited by 6
Aging and Altered Gravity: A Cellular Perspective
van Rijthoven, S., & van Loon, J. J. W. A. (2025) · The FASEB Journal · doi:10.1096/fj.202402989r · Cited by 5
Culture of human cells in experimental units for spaceflight impacts on their behavior
Cazzaniga, A., Moscheni, C., Maier, J. A. M., & Castiglioni, S. (2016) · Experimental Biology and Medicine · doi:10.1177/1535370216684039 · Cited by 5
Increased bone marrow adiposity in murine femoro-tibial epiphyses exposed to 30 days of microgravity
Endicott, J., & Fitzgerald, J. (2019) · Matters Select · doi:10.19185/matters.201904000010 · Cited by 5
Pathophysiologic Spine Adaptations and Countermeasures for Prolonged Spaceflight
Schlaff, C. D., Helgeson, M. D., & Wagner, S. C. (2023) · Clinical Spine Surgery A Spine Publication · doi:10.1097/bsd.0000000000001488 · Cited by 4
Meas, S. J., Daire, G. M., Friedman, M. A., DeNapoli, R. C., Ghosh, P., Farr, J. N., & Donahue, H. J. (2023) · Bone · doi:10.1016/j.bone.2023.116973 · Cited by 4
Effects of Aging and Exercise Training on Bone Vascular Function and Structural Adaptations
Park, H., Narayanan, S. A., Caldwell, J. T., Behnke, B. J., Muller‐Delp, J. M., & Delp, M. D. (2024) · Bone · doi:10.1016/j.bone.2024.117335 · Cited by 4
Synchrotron X-Ray Phase Nanotomography for Bone Tissue Characterization
Varga, P., Weber, L., Hesse, B., & Langer, M. (2016) · Springer eBooks · doi:10.1007/978-3-662-48606-1_1 · Cited by 4
Новиков, В. Е., Рыкова, М. П., Антропова, Е. Н., Берендеева, Т. А., Kalinin, S. A., Vassilieva, G. Y., & Ponomarev, S. A. (2017) · Human Physiology · doi:10.1134/s0362119717060068 · Cited by 4
Zhang, S., Ueno, D., Ohira, T., Kato, H., Izawa, T., Yamanouchi, S., Yoshida, Y., Takahashi, A., & Ohira, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.707470 · Cited by 4
Coulombe, J. C., Mullen, Z., Weins, A. M., Fisher, L. E., Lynch, M. E., Stodieck, L., & Ferguson, V. L. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.04.474962 · Cited by 4
The influence of age and mechanical loading on bone structure and mechanical properties
Aido, M. (2015) · MPG.PuRe (Max Planck Society) · doi:10.14279/depositonce-4511 · Cited by 4
BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling
Zhao, H., Yang, M., Liu, Y., Tu, X., & Shao, G. (2025) · npj Microgravity · doi:10.1038/s41526-025-00510-y · Cited by 3
Tosi, D. D., Tiberio, F., Pietro, L. D., Polito, L., Parolini, O., Minotti, A., Arcovito, A., & Lattanzi, W. (2026) · npj Microgravity · doi:10.1038/s41526-025-00556-y · Cited by 4
Role and mechanism of histone demethylase PHF8 in weightlessness osteoporosis
Ding, Z., Wang, D., Zhang, S., Yang, X., Xu, M., Li, W., Shi, Q., Gao, B., Wang, Y., & Yan, M. (2024) · Experimental Cell Research · doi:10.1016/j.yexcr.2024.114270 · Cited by 3
Liu, T., Melkus, G., Ramsay, T., Berthiaume, A., Armbrecht, G., & Trudel, G. (2025) · Experimental Physiology · doi:10.1113/ep091495 · Cited by 3
Bioinformatic analysis of the RNA expression patterns in microgravity-induced bone loss
Zhang, X., Xue, T., Hu, Z., Guo, X., Li, G., Wang, Y., Zhang, L., Xu, L., Cao, X., Zhang, S., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.985025 · Cited by 3
Sahbani, K., Pan, J., Zaidi, M., Cardozo, C. P., Bauman, W. A., & Tawfeek, H. A. W. (2023) · The FASEB Journal · doi:10.1096/fj.202300109rr · Cited by 4
Nieves, J. G., Nietzer, S., Lechner, L., Weigel, T., Bauer, L., Fuchs, K., Remmert, C., Putz, E., Kuhn, S. P., Hudecek, M., et al. (2025) · Materialia · doi:10.1016/j.mtla.2025.102440 · Cited by 2
Soluble RANKL exaggerates hindlimb suspension‐induced osteopenia but not muscle protein balance
Speacht, T. L., Lang, C. H., & Donahue, H. J. (2020) · Journal of Orthopaedic Research® · doi:10.1002/jor.24917 · Cited by 2
Markina, E. A., Andreeva, E. R., & Buravkova, L. B. (2025) · Cells Tissues Organs · doi:10.1159/000545284 · Cited by 1
Bone mineral loss damages renal tubules in mice
Hayashi, H., Miura, Y., Iwazu, Y., Mukai, H., Mori, Y., Kuchimaru, T., Ohno, N., Aiba, T., Okada, R., Kamimura, D., et al. (2026) · Communications Biology · doi:10.1038/s42003-026-09603-0 · Cited by 1
Schemenz, V., Rummler, M., van Tol, A. F., Vitienes, I., Thiele, T., Hartmann, M. A., Burghammer, M. C., Fratzl, P., Weinkamer, R., Willie, B. M., et al. (2026) · Advanced NanoBiomed Research · doi:10.1002/anbr.202500169 · Cited by 1
The SCD – Stem Cell Differentiation ESA Project: Preparatory Work for the Spaceflight Mission
Versari, S., Barenghi, L., van Loon, J. J. W. A., & Bradamante, S. (2015) · Microgravity Science and Technology · doi:10.1007/s12217-015-9466-5 · Cited by 1
Ouyang, X., Liu, J.-Y., Li, Z., Zhu, J., Li, W., He, R., Liu, S., Xue, Y., Huang, C., Zhang, J., et al. (2025) · World Journal of Traditional Chinese Medicine · doi:10.4103/wjtcm.wjtcm_21_25 · Cited by 1
Palle, J., Wittig, N. K., Østergaard, M., Jensen, A. B., & Birkedal, H. (2019) · Developments in X-Ray Tomography · doi:10.1117/12.2531980 · Cited by 1
Markina, E. A., Alekseeva, O. Y., Andreeva, E. R., & Buravkova, L. B. (2021) · Stem Cells and Development · doi:10.1089/scd.2021.0111 · Cited by 1
Bukreeva, I., Gulimova, V. I., Krivonosov, Y. S., Buzmakov, A. V., Junemann, O., Cedola, A., Fratini, M., Maugeri, L., Provinciali, G. B., Palermo, F., et al. (2023) · Cells · doi:10.3390/cells12192415 · Cited by 1
Demir, Ö., Çam, E., Yolver, B., Kolay, K., Sakar, M. R., Aydin, B., & Taştan, C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.12.675876 · Cited by 1
Santhanam, A., Momin, Z., Qin, X., Wang, Q., Krishnavajhala, A., Jiang, Q., Walker, K., Kalra, D., Gingras, M., Chao, H., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.01.729304 · Cited by 1
The Role of Nitric Oxide in Skeletal Simulated Microgravity Response
Tran, L. H. (2015) · San José State University ScholarWorks (San Jose State University) · doi:10.31979/etd.w92s-dmy3
Osteocytes: More Than Just Mechanosensory Cells
Prideaux, M., & Bonewald, L. F. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.11203-6
Microgravity effects and aging physiology: similar changes or common mechanisms?
Ratushnyy, A. Y., & Buravkova, L. B. (2023) · Biochemistry (Moscow) · doi:10.31857/s032097252311009x
Hao, W., Yun, C., Patil, S., Qiu, W., Qian, A., & Xiao, L. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-36338-2.00020-1
Meas, S. J., Daire, G. M., Buettmann, E. G., & Donahue, H. J. (2025) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2025.113134
Osorio, R., Aguilera, F. S., Fernández-Romero, E., Toledano, R., Osorio, M. T., Manuel, T., & Osorio, E. (2026) · Journal of Dentistry · doi:10.1016/j.jdent.2026.106340
Hirayama, J., Takino, H., Maruyama, Y., Ikegame, M., Tabuchi, Y., Matsubara, H., Shimizu, N., Mishima, H., Hattori, A., & Suzuki, N. (2026) · Biological Sciences in Space · doi:10.2187/bss.40.1
Spaceflight and the rodent skeleton: A perspective
Monfared, V., Iwaniec, U. T., & Turner, R. T. (2026) · Bone Reports · doi:10.1016/j.bonr.2026.101917
Oral bone tissue adaptation in space: Unraveling biological responses to microgravity
Liu, B., Chen, X., Peng, Z., & Peng, Q. (2026) · Acta Astronautica · doi:10.1016/j.actaastro.2026.05.001
Languisse, F. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.6660400
Hypergravity reduces F-actin accumulation in osteoclasts, with attenuated bone resorption
Takahashi, N., Fujita, A., Azetsu, Y., Karakawa, A., Myers, M., Takami, M., & Chatani, M. (2026) · PLoS ONE · doi:10.1371/journal.pone.0351542
Comprehensive Analysis of Rat Bone Tissue after 7 and 21 Days of Hindlimb Unloading
Lukicheva, N. A., Gordienko, K. V., Korotkova, N. A., Doronina, M. S., Baranovskaya, V. B., & Vassilieva, G. Y. (2026) · Journal of Evolutionary Biochemistry and Physiology · doi:10.1134/s0022093026030014
Ji, G., Zhou, Z., Xia, H., Zhao, Z., & Lu, H. (2026) · npj Microgravity · doi:10.1038/s41526-026-00651-8
Beyond Earth: Recent Advancements in Microgravity Biomedical and Genetic Research in Saudi Arabia
Hawsawi, Y. M., Jamous, Y. F., Alghannam, S. F., Aldahshan, H., Alothman, L., Fitaihi, R. A., Aljawini, N., Alqarni, S. S., Alfaraj, R., Aldkheil, E. A., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27177613
Ahmad, U. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1887696
Microgravity-Induced Osteocyte Dysfunction: An Osteocyte Mechanobiology Unit Framework
Roque, A. M., Acosta, O. B. L., Lozano, M. J. R., Suarez, H. N., & Bacabac, R. G. (2026) · Current Osteoporosis Reports · doi:10.1007/s11914-026-00989-8
Wei, F., Ngo, C., Neal, C. J., Schwartzman, J. D., Pugazhendhi, A. S., Omer, M., Walck, C. D., Seal, S., & Coathup, M. J. (2026) · Metabolism · doi:10.1016/j.metabol.2026.156794
Vlasova, D. D., Sadova, A. A., Шмаров, В. А., Shigueva, T. A., Rukavishnikov, I. V., Tomilovskaya, E., Ljubisavljević, M., Sarhan, D., & Kuznetsov, N. V. (2026) · Cells · doi:10.3390/cells15212017
Metadata
- Authors
- Blaber, E. A., Dvorochkin, N., Lee, C., Alwood, J. S., Yousuf, R., Pianetta, P., Globus, R. K., Burns, B. P., & Almeida, E. A. C.
- Affiliations
- Ames Research Center (US)
- SLAC National Accelerator Laboratory (US)
- UNSW Sydney (AU)
- Corresponding author
- Almeida, Eduardo A. C.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-04-18
- Volume
- 8
- Issue
- 4
- Pages
- e61372
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3630201
- OpenAlex
- W2142491318
- Cited by
- 196
- References
- 74
- Citations by year
- 2026: 15 · 2025: 17 · 2024: 10 · 2023: 9 · 2022: 9 · 2021: 21 · 2020: 21 · 2019: 16 · 2018: 19 · 2017: 11 · 2016: 15 · 2015: 12 · 2014: 19 · 2013: 2
- FWCI
- 11.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, public-domain)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, public-domain)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Aeronautics and Space Administration
- Topics
- Health Sciences › Medicine › Physiology (medical) › Spaceflight effects on biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- microgravity, spaceflight, bone loss, osteoclasts, osteoblasts, CDKN1A/p21, Trp53, matrix metalloproteinases, osteocytes, bone resorption, trabecular bone, bone unloading, female mice, bone homeostasis
- MeSH terms
- Animals, Bone and Bones, Bone Resorption, Female, Mice, Inbred C57BL, Osteoclasts, Osteocytes, Osteolysis, Pelvic Bones, Weightlessness, Cyclin-Dependent Kinase Inhibitor p21, Mice, X-Ray Microtomography, Cell Cycle Checkpoints
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
