Effects of N-acetylcysteine in ozone-induced chronic obstructive pulmonary disease model
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INTRODUCTION: Chronic exposure to high levels of ozone induces emphysema and chronic inflammation in mice. We determined the recovery from ozone-induced injury and whether an antioxidant, N-acetylcysteine (NAC), could prevent or reverse the lung damage. METHODS: Mice were exposed to ozone (2.5 ppm, 3 hours/12 exposures, over 6 weeks) and studied 24 hours (24h) or 6 weeks (6W) later. Nac (100 mg/kg, intraperitoneally) was administered either before each exposure (preventive) or after completion of exposure (therapeutic) for 6 weeks. RESULTS: After ozone exposure, there was an increase in functional residual capacity, total lung volume, and lung compliance, and a reduction in the ratio of forced expiratory volume at 25 and 50 milliseconds to forced vital capacity (FEV25/FVC, FEV50/FVC). Mean linear intercept (Lm) and airway hyperresponsiveness (AHR) to acetylcholine increased, and remained unchanged at 6W after cessation of exposure. Preventive NAC reduced the number of BAL macrophages and airway smooth muscle (ASM) mass. Therapeutic NAC reversed AHR, and reduced ASM mass and apoptotic cells. CONCLUSION: Emphysema and lung function changes were irreversible up to 6W after cessation of ozone exposure, and were not reversed by NAC. The beneficial effects of therapeutic NAC may be restricted to the ASM.
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Rodent studies confirmed that the use of N-acetylcysteine and sulfide salt can help prevent or recover the lung impairment caused by ozone.
“For example, rodent studies confirmed that the use of N-acetylcysteine and sulfide salt can help prevent or recover the lung impairment caused by ozone (82, 83).”
From Ozone pollution: A Major health hazard worldwide (2019) · cites this paper as [83]
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- Authors
- Li, F., Wiegman, C., Seiffert, J. M., Zhu, J., Clarke, C., Chang, Y., Bhavsar, P., Adcock, I., Zhang, J., Zhou, X., & Chung, K. F.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Imperial College London (GB)
- University of Southern California (US)
- Corresponding author
- Chung, Kian Fan
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-11-18
- Volume
- 8
- Issue
- 11
- Pages
- e80782
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3832609
- OpenAlex
- W2014519750
- Cited by
- 62
- References
- 29
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 5 · 2021: 9 · 2020: 6 · 2019: 4 · 2018: 6 · 2017: 7 · 2016: 5 · 2015: 2 · 2014: 1
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Spiral (Imperial College London) (submittedVersion, other-oa)
- Funders
- European Respiratory Society
- Wellcome Trust (083905)
- Lloyd's Tercentenary Research Foundation
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Keywords
- N-acetylcysteine, ozone exposure, chronic obstructive pulmonary disease, emphysema, airway hyperresponsiveness, airway smooth muscle, lung function, macrophages, apoptosis, antioxidant therapy, chronic inflammation
- MeSH terms
- 8-Hydroxy-2'-Deoxyguanosine, Acetylcysteine, Animals, Bronchoalveolar Lavage Fluid, Deoxyguanosine, Disease Models, Animal, Emphysema, Expectorants, Lung, Malondialdehyde, Ozone, Respiratory Function Tests, Gene Expression, Bronchial Hyperreactivity, Apoptosis, Pulmonary Disease, Chronic Obstructive, Mice
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