Neuronal synapse formation induced by microglia and interleukin 10
Open accessAbstract
Recently, it was found that microglia regulated synaptic remodeling of the developing brain, but their mechanisms have not been well understood. In this study, the action of microglia on neuronal synapse formation was investigated, and the primary target of microglial processes was discovered. When the developing microglia were applied to cultured hippocampal neurons without direct contact, the numbers of dendritic spines and excitatory and inhibitory synapses significantly increased. In order to find out the main factor for synaptic formation, the effects of cytokines released from microglia were examined. When recombinant proteins of cytokines were applied to neuronal culture media, interleukin 10 increased the numbers of dendritic spines in addition to excitatory and inhibitory synapses. Interestingly, without external stimuli, the amount of interleukin 10 released from the intact microglia appeared to be sufficient for the induction of synaptic formation. The neutralizing antibodies of interleukin 10 receptors attenuated the induction of the synaptic formation by microglia. The expression of interleukin 10 receptor was newly found in the hippocampal neurons of early developmental stage. When interleukin 10 receptors on the hippocampal neurons were knocked down with specific shRNA, the induction of synaptic formation by microglia and interleukin 10 disappeared. Pretreatment with lipopolysaccharide inhibited microglia from inducing synaptic formation, and interleukin 1β antagonized the induction of synaptic formation by interleukin 10. In conclusion, the developing microglia regulated synaptic functions and neuronal development through the interactions of the interleukin 10 released from the microglia with interleukin 10 receptors expressed on the hippocampal neurons.
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Addition of microglia to hippocampal cultures increases the number of both excitatory and inhibitory synapses through their release of IL-10.
“Addition of microglia to hippocampal cultures increases the number of both excitatory and inhibitory synapses through their release of IL-10 (Lim et al., 2013).”
From Adult neurogenesis, glia, and the extracellular matrix (2019) · cites this paper as Lim et al., 2013
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- Authors
- Lim, S.-H., Park, E., You, B., Jung, Y., Park, A.-R., Park, S. G., & Lee, J.-R.
- Affiliations
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Corresponding author
- Lee, Jae-Ran
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-11-22
- Volume
- 8
- Issue
- 11
- Pages
- e81218
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3838367
- OpenAlex
- W2035557202
- Cited by
- 224
- References
- 49
- Citations by year
- 2026: 10 · 2025: 14 · 2024: 19 · 2023: 18 · 2022: 19 · 2021: 33 · 2020: 19 · 2019: 23 · 2018: 17 · 2017: 17 · 2016: 14 · 2015: 9 · 2014: 12
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- National Research Foundation
- Korea Research Institute of Bioscience and Biotechnology
- National Research Foundation of Korea (2012R1A2A2A02014520)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- microglia, synaptogenesis, IL-10, interleukin-10 receptor, dendritic spines, excitatory and inhibitory synapses, hippocampal neurons, neuronal development, lipopolysaccharide, IL-1β, synaptic remodeling
- MeSH terms
- Animals, Animals, Newborn, Hippocampus, Neuroeffector Junction, Gene Expression, Interleukin-10, Microglia, Pyramidal Cells, Rats, Interleukin-1beta, Receptors, Interleukin-10, Gene Knockdown Techniques
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