Orally Administrated Lactobacillus pentosus var. plantarum C29 Ameliorates Age-Dependent Colitis by Inhibiting the Nuclear Factor-Kappa B Signaling Pathway via the Regulation of Lipopolysaccharide Production by Gut Microbiota
Open accessAbstract
To evaluate the anti-inflammaging effect of lactic acid bacteria (LAB) on age-dependent inflammation, we first screened and selected a tumor necrosis factor (TNF)-α and reactive oxygen species (ROS)-inhibitory LAB, Lactobacillus pentosus var. plantarum C29, among the LABs isolated from fermented vegetables using LPS-stimulated mouse peritoneal macrophages. Oral administration of C29 (2 × 109 CFU/rat) for 8 weeks in aged Fischer 344 rats (age, 16 months) inhibited the expression of the inflammatory markers myeloperoxidase, inducible nitric oxide (NO) synthase, cyclooxygenase-2, pro-inflammatory cytokines tumor necrosis factor (TNF)-α and IL-6 and the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), activator protein 1 (AP1), and mitogen-activated protein kinases (MAPKs). Treatment with C29 induced the expression of tight junction proteins ZO-1, occludin, and claudin-1, and reduced intestinal microbial LPS and plasmatic LPS levels and ROS, as well as the Firmicutes to Bacteroidetes ratio, which is significantly higher in aged rats than in young rats. C29 treatment also reduced plasmatic reactive oxygen species, malondialdehyde, C-reactive protein, and TNF-α, and suppressed expression of senescence markers p16 and p53 in the colon of the aged rats, but increased SIRT 1 expression. Based on these findings, we concluded that C29 treatment may suppress aging-dependent colitis by inhibiting NF-κB, AP1, and MAPK activation via the inhibition of gut microbiota LPS production and the induction of tight junction protein expression.
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Intake of probiotics, such as Lactobacillus pentosus var. plantarum C29, significantly reduces the level of systemic inflammatory factors and the expression of aging markers p16 and p53.
“Previous studies have also proved that the intake of probiotics, such as Lactobacillus pentosus var. plantarum C29, has been proved to significantly reduce the level of systemic inflammatory factors and the expression of aging markers p16 and p53.325”
From Inflammation and aging: signaling pathways and intervention therapies (2023) · cites this paper as [325]
The Results sections on plasma and colon report that oral C29 reduced several circulating inflammatory markers and suppressed colonic p16 and p53 expression in aged F344 rats (page not provided). The claim omits that animal and tissue-specific scope, and its broad reference to probiotics is not established by this C29 study. (LLM, conf=0.84)
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Inflammation and aging: signaling pathways and intervention therapies
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“plantarum C29, has been proved to significantly reduce the level of systemic inflammatory factors and the expression of aging markers p16 and p53.(325) Similarly, consumption of polyunsaturated fatty acids has been shown to significantly reduce levels of inflammatory cytokines throughout the body(326) The continuous i…”
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Self-citation
- Authors
- Jeong, J.-J., Kim, K.-A., Jang, S.-E., Woo, J.-Y., Han, M. J., & Kim, D.-H.
- Affiliations
- Kyung Hee University (KR)
- Corresponding author
- Kim, Dong-Hyun
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-02-17
- Volume
- 10
- Issue
- 2
- Pages
- e0116533
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4331539
- OpenAlex
- W2081611768
- Cited by
- 86
- References
- 39
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 8 · 2023: 8 · 2022: 8 · 2021: 5 · 2020: 10 · 2019: 6 · 2018: 10 · 2017: 10 · 2016: 12 · 2015: 3
- FWCI
- 15.61
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-11-12)
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- National Research Foundation (2013M3A9B6076413)
- Ministry of Science, ICT and Future Planning (2013M3A9B6076413)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- lactic acid bacteria, aging, NF-κB signaling, lipopolysaccharide, gut microbiota, tight junction proteins, reactive oxygen species, TNF-α, senescence, SIRT1, Firmicutes/Bacteroidetes ratio, anti-inflammatory activity, fermented vegetables
- MeSH terms
- Gastrointestinal Microbiome, Administration, Oral, Animals, Bacteria, Body Weight, Colitis, Colon, Lactobacillus, Lipopolysaccharides, Male, Mice, Inbred C57BL, Rats, Inbred F344, RNA, Ribosomal, 16S, Signal Transduction, Cytokines, NF-kappa B, Reactive Oxygen Species, Macrophages, Peritoneal, Mice, Rats, Cyclooxygenase 2, Nitric Oxide Synthase Type II
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