The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots
Open accessAbstract
As a new technology for gene editing, the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) system has been rapidly and widely used for genome engineering in various organisms. In the present study, we successfully applied type II CRISPR/Cas9 system to generate and estimate genome editing in the desired target genes in soybean (Glycine max (L.) Merrill.). The single-guide RNA (sgRNA) and Cas9 cassettes were assembled on one vector to improve transformation efficiency, and we designed a sgRNA that targeted a transgene (bar) and six sgRNAs that targeted different sites of two endogenous soybean genes (GmFEI2 and GmSHR). The targeted DNA mutations were detected in soybean hairy roots. The results demonstrated that this customized CRISPR/Cas9 system shared the same efficiency for both endogenous and exogenous genes in soybean hairy roots. We also performed experiments to detect the potential of CRISPR/Cas9 system to simultaneously edit two endogenous soybean genes using only one customized sgRNA. Overall, generating and detecting the CRISPR/Cas9-mediated genome modifications in target genes of soybean hairy roots could rapidly assess the efficiency of each target loci. The target sites with higher efficiencies can be used for regular soybean transformation. Furthermore, this method provides a powerful tool for root-specific functional genomics studies in soybean.
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Cites
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Multiplex Genome Engineering Using CRISPR/Cas Systems
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Cited by
Shi, J., Gao, H., Wang, H., Lafitte, H. R., Archibald, R. L., Yang, M., Hakimi, S. M., Mo, H., & Habben, J. E. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12603 · Cited by 1,029
CRISPR for Crop Improvement: An Update Review
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 619
Research Progress and Perspective on Drought Stress in Legumes: A Review
Nadeem, M., Li, J., Yahya, M., Sher, A., Ma, C., Wang, X., & Qiu, L. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20102541 · Cited by 409
Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants
Osakabe, Y., Watanabe, T., Sugano, S. S., Ueta, R., Ishihara, R., Shinozaki, K., & Osakabe, K. (2016) · Scientific Reports · doi:10.1038/srep26685 · Cited by 367
CRISPR/Cas9‐mediated efficient targeted mutagenesis in grape in the first generation
Wang, X., Tu, M., Wang, D., Liu, J., Li, Y., Li, Z., Wang, Y., & Wang, X. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12832 · Cited by 367
Metadata
- Authors
- Cai, Y., Chen, L., Liu, X., Sun, S., Wu, C., Jiang, B., Han, T., & Hou, W.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-08-18
- Volume
- 10
- Issue
- 8
- Pages
- e0136064
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1887675732
- Cited by
- 273
- References
- 28
- FWCI
- 7.98
- Type
- journal article
- Language
- en
- Open access
- gold
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