ARGOS 8 variants generated by CRISPR ‐Cas9 improve maize grain yield under field drought stress conditions
Open accessAbstract
Maize ARGOS8 is a negative regulator of ethylene responses. A previous study has shown that transgenic plants constitutively overexpressing ARGOS8 have reduced ethylene sensitivity and improved grain yield under drought stress conditions. To explore the targeted use of ARGOS8 native expression variation in drought-tolerant breeding, a diverse set of over 400 maize inbreds was examined for ARGOS8 mRNA expression, but the expression levels in all lines were less than that created in the original ARGOS8 transgenic events. We then employed a CRISPR-Cas-enabled advanced breeding technology to generate novel variants of ARGOS8. The native maize GOS2 promoter, which confers a moderate level of constitutive expression, was inserted into the 5'-untranslated region of the native ARGOS8 gene or was used to replace the native promoter of ARGOS8. Precise genomic DNA modification at the ARGOS8 locus was verified by PCR and sequencing. The ARGOS8 variants had elevated levels of ARGOS8 transcripts relative to the native allele and these transcripts were detectable in all the tissues tested, which was the expected results using the GOS2 promoter. A field study showed that compared to the WT, the ARGOS8 variants increased grain yield by five bushels per acre under flowering stress conditions and had no yield loss under well-watered conditions. These results demonstrate the utility of the CRISPR-Cas9 system in generating novel allelic variation for breeding drought-tolerant crops.
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CRISPR-Cas9 has been successfully applied to crop plants to alter agriculturally important traits such as disease resistance and drought tolerance.
“The technique has already been successfully applied to crop plants to alter agriculturally important traits such as disease resistance (Wang et al., 2014) and drought tolerance (Shi et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Shi et al., 2017
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- Authors
- Shi, J., Gao, H., Wang, H., Lafitte, H. R., Archibald, R. L., Yang, M., Hakimi, S. M., Mo, H., & Habben, J. E.
- Affiliations
- Pioneer Hi-Bred (US)
- Corresponding author
- Shi, Jinrui
- ORCID
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2016-08-17
- Volume
- 15
- Issue
- 2
- Pages
- 207-216
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- PMC
- PMC5258859
- OpenAlex
- W2488393959
- Cited by
- 1,029
- References
- 56
- Citations by year
- 2026: 97 · 2025: 119 · 2024: 99 · 2023: 127 · 2022: 157 · 2021: 145 · 2020: 109 · 2019: 85 · 2018: 47 · 2017: 40 · 2016: 3 · 2014: 1
- FWCI
- 30.33
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Biotechnology Journal (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- maize, drought stress, grain yield, CRISPR/Cas9, ethylene response, promoter replacement, allelic variation, drought tolerance breeding, gene expression
- MeSH terms
- Gene Editing, Base Sequence, Edible Grain, Zea mays, Ethylenes, Plant Proteins, Promoter Regions, Genetic, RNA, Messenger, Stress, Physiological, Polymerase Chain Reaction, Gene Expression Regulation, Plant, Crops, Agricultural, Plants, Genetically Modified, Biodiversity, Droughts, CRISPR-Cas Systems, CRISPR-Associated Proteins
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