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Cited by 102Oral microbiology and periodontitis researchPeriodontal Regeneration and TreatmentsDental Research and COVID-19

Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola / Prevotella intermedia Co-Infection Are Associated with Severe Periodontitis in a Thai Population

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Torrungruang, Kitti, Jitpakdeebordin, Supawadee, Charatkulangkun, Orawan, Gleebbua, Yingampa·2015·PLOS ONE
DOI 10.1371/journal.pone.0136646PMC PMC4552424
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Abstract

Periodontitis is a polymicrobial infection of tooth-supporting tissues. This cross-sectional study aimed to examine the associations between five target species and severe periodontitis in a Thai population. Using the CDC/AAP case definition, individuals diagnosed with no/mild and severe periodontitis were included. Quantitative analyses of Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Treponema denticola (Td), and Prevotella intermedia (Pi) in subgingival plaque were performed using real-time polymerase chain reaction. The association between target species and severe periodontitis was examined using logistic regression analysis. The study subjects comprised 479 individuals with no/mild periodontitis and 883 with severe periodontitis. Bacterial prevalence and quantity were higher in subjects with severe periodontitis than in those with no/mild disease. In the fully adjusted model, all species except Tf showed a dose-dependent relationship with periodontitis. The mere presence of Pg, even in low amount, was significantly associated with severe periodontitis, while the amount of Aa, Td, and Pi had to reach the critical thresholds to be significantly associated with disease. Compared to individuals with low levels of both Td and Pi, high colonization by either Td or Pi alone significantly increased the odds of having severe periodontitis by 2.5 (95%CI 1.7-3.5) folds. The odds ratio was further increased to 14.8 (95%CI 9.2-23.8) in individuals who were highly colonized by both species. Moreover, the presence of Pg and high colonization by Aa were independently associated with severe periodontitis with odds ratios of 5.6 (95%CI 3.4-9.1) and 2.2 (95%CI 1.5-3.3), respectively. Our findings suggest that the presence of Pg and high colonization by Aa, Td, and Pi play an important role in severe periodontitis in this study population. We also demonstrate for the first time that individuals co-infected with Td and Pi were more likely to have periodontitis than were those infected with a single pathogen.

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  • Based on reported target strain abundance in saliva (<10^7 cells/mL for each target species) and average saliva volume, the total amount of postbiotic cells required for efficient coaggregation was estimated to be 10^9 bacterial cells/g of product.

    “Based on the reported target strain abundance in saliva (<107 cells/mL saliva for each of the target species) [43–46] and the average saliva volume in the mouth under stimulated conditions [47,48], we estimated that the total amount of postbiotic cells required for efficient coaggregation would be 109 bacterial cells/g of product.”

    From Coaggregation of oral pathogens by postbiotic lactobacilli (2025) · cites this paper as [46]

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Authors
Torrungruang, K., Jitpakdeebordin, S., Charatkulangkun, O., & Gleebbua, Y.
Affiliations
  • Chulalongkorn University (TH)
  • Mahidol University (TH)
  • Electricity Generating Authority of Thailand (TH)
Corresponding author
Torrungruang, Kitti
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2015-08-27
Volume
10
Issue
8
Pages
e0136646
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0136646
PMC
PMC4552424
OpenAlex
W2216179511
Cited by
102
References
34
Citations by year
2026: 8 · 2025: 14 · 2024: 12 · 2023: 4 · 2022: 12 · 2021: 9 · 2020: 15 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 7
FWCI
9.47
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Chiang Mai University
  • Chulalongkorn University
  • National Research Council of Thailand
  • Electricity Generating Authority of Thailand
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
  • Health Sciences › Dentistry › General Dentistry › Dental Research and COVID-19
Keywords
severe periodontitis, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Treponema denticola, Prevotella intermedia, Tannerella forsythia, co-infection, subgingival plaque, bacterial colonization, dose-response relationship, Thai population
MeSH terms
Adult, Bacterial Infections, Female, Humans, Male, Middle Aged, Severity of Illness Index, Thailand, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Treponema denticola, Chronic Periodontitis, Coinfection

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