Cited by 144Milk Quality and Mastitis in Dairy CowsProbiotics and Fermented FoodsRuminant Nutrition and Digestive Physiology

Lactic Acid Bacteria Isolated from Bovine Mammary Microbiota: Potential Allies against Bovine Mastitis

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Bouchard, Damien S., Seridan, Bianca, Saraoui, Taous, Rault, Lucie, Germon, Pierre, Gonzalez-Moreno, Candelaria, Nader-Macias, Fatima M. E., Baud, Damien +7 more·2015·PLOS ONE

Abstract

Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.

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  • L. helveticus 34.9 can be considered to have a medium hydrophobic character according to MATH classification.

    “In this case, L. helveticus 34.9 can be considered to have a medium hydrophobic character.”

    From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [33]

    The Results section on surface properties (page unavailable) classifies MATH values between 33% and 66% as medium hydrophobicity. It does not report a MATH value or classification for L. helveticus 34.9, so it supports the classification criterion but not its application to that strain. (LLM, conf=0.85)

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Research works printed but not linked: 1

  1. [5]Even S, Bouchard D, Le Loir Y. Lactic acid bacteria to modulate virulence expression in pathogenic bacteria: An alternative to killing? Interactive probiotics CRC Press Boca Raton, USA: Pessione E. (Eds.); 2014 pp. 52– 80.

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Authors
Bouchard, D. S., Seridan, B., Saraoui, T., Rault, L., Germon, P., Gonzalez-Moreno, C., Nader-Macias, F. M. E., Baud, D., François, P., Chuat, V., Chain, F., Langella, P., Nicoli, J., Le Loir, Y., & Even, S.
Affiliations
  • Institut Agro Rennes-Angers (FR)
  • Infectiologie et santé publique (FR)
  • University Hospital of Geneva (CH)
  • Microbiologie de l’alimentation au service de la santé (FR)
  • Universidade Federal de Minas Gerais (BR)
Corresponding author
Even, Sergine
Venue
PLOS ONE
Published
2015-12-29
Volume
10
Issue
12
Pages
e0144831
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2219521950
Cited by
144
References
40
Citations by year
2026: 5 · 2025: 10 · 2024: 23 · 2023: 15 · 2022: 15 · 2021: 20 · 2020: 16 · 2019: 6 · 2018: 12 · 2017: 15 · 2016: 7
FWCI
24.59
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
  • HAL (submittedVersion, cc-by)
  • Americanae (AECID Library) (submittedVersion, cc-by-nc-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-sa)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
  • Greater South Information System
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Institut National de la Recherche Agronomique
  • Campus France
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Milk Quality and Mastitis in Dairy Cows
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Keywords
bovine mastitis, lactic acid bacteria, Lactobacillus brevis, Lactobacillus plantarum, Lactococcus, mastitis prevention, competitive exclusion, anti-inflammatory activity, IL-8 secretion, Staphylococcus aureus, Escherichia coli, Streptococcus uberis, hydrophobicity, self-aggregation, antibiotic resistance genes, dairy cattle
MeSH terms
Animals, Bacterial Adhesion, Bacterial Infections, Cattle, Female, Humans, Lactobacillus, Mammary Glands, Animal, Mastitis, Bovine, Milk, Molecular Sequence Data, RNA, Bacterial, RNA, Ribosomal, 16S, Genome, Bacterial, Lactococcus, Sequence Analysis, DNA, HT29 Cells, Molecular Typing, Microbiota

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