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Cited by 18Bone Tissue Engineering MaterialsBone Metabolism and DiseasesMedicinal Plant Pharmacodynamics Research

Osteoinductive Effects of Free and Immobilized Bone Forming Peptide-1 on Human Adipose-Derived Stem Cells

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Li, Wenyue, Zheng, Yunfei, Zhao, Xianghui, Ge, Yanjun, Chen, Tong, Liu, Yunsong, Zhou, Yongsheng·2016·PLOS ONE
DOI 10.1371/journal.pone.0150294PMC PMC4773240
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Abstract

Most synthetic polymeric materials currently used for bone tissue engineering lack specific signals through which cells can identify and interact with the surface, resulting in incompatibility and compromised osteogenic activity. Soluble inductive factors also have issues including a short half-live in vivo. Bone forming peptide-1 is a truncated peptide from the immature form of bone morphogenetic protein-7 (BMP-7) that displays higher osteogenic activity than full-length, mature BMP-7. In this study, we used a mussel-inspired immobilization strategy mediated by polymerization of dopamine to introduce recently discovered stimulators of bone forming peptide-1 (BFP-1) onto the surface of poly-lactic-co-glycolic acid (PLGA) substrate to form a biomaterial that overcomes these challenges. Human adipose-derived stem cells (hASCs), being abundant and easy accessible, were used to test the osteogenic activity of BFP-1 and the novel biomaterial. Under osteoinductive conditions, cells treated with both BFP-1 alone and BFP-1-coated biomaterials displayed elevated expression of the osteogenic markers alkaline phosphatase (ALP), osteocalcin (OC), and RUNX2. Furthermore, hASCs associated with poly-dopamine-assisted BFP-1-immobilized PLGA (pDA-BFP-1-PLGA) scaffolds promoted in vivo bone formation in nude mice. Our novel materials may hold great promise for future bone tissue engineering applications.

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  • Nano-HA modified PCL via PDA coating enhanced cell adhesion and proliferation.

    “The results showed that nano-HA modified PCL via PDA coating enhanced the cell adhesion and proliferation”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [86]

    The Results and Discussion section, “Adhesion and proliferation of cells on PLGA substrates” (page unavailable), reports greater cell adhesion and proliferation on polydopamine-modified films than on unmodified films. But the tested material was PLGA, with or without immobilized BFP-1—not nano-HA-modified PCL. The source therefore supports the general effect of the coating, not the specific material claimed. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Nano-HA modified PCL via PDA coating promoted bone regeneration even in the absence of osteogenesis soluble inducing factors in in vivo tests.

    “as well as promoting the bone regeneration even in the absence of osteogenesis soluble inducing factors in in vivo tests.”

    From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [86]

    The Results and Discussion (Histological assessment of bone formation in vivo; page unavailable) report superior ectopic bone formation with hASC-loaded, pDA-coated scaffolds. But the tested material was BFP-1-immobilized PLGA, not nano-HA-modified PCL. The Animal experiments methods also state that the cell-loaded scaffolds were cultured in osteogenic medium before implantation, so the claimed absence of soluble inducing factors is not established for the full experiment. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Li, W., Zheng, Y., Zhao, X., Ge, Y., Chen, T., Liu, Y., & Zhou, Y.
Affiliations
Peking University (CN)
Corresponding author
  • Liu, Yunsong
  • Zhou, Yongsheng
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2016-03-01
Volume
11
Issue
3
Pages
e0150294
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0150294
PMC
PMC4773240
OpenAlex
W2291489888
Cited by
18
References
2
Citations by year
2024: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3
FWCI
1.29
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Greater South Information System
Funders
  • National Natural Science Foundation of China (2011/81170937, 81400484, 81170937)
  • Ministry of Education of the People's Republic of China (NCET-11-0026)
  • Ministry of Education, India
  • Program for New Century Excellent Talents in University (NCET‐11‐0026, NCET-11-)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
Keywords
BMP-7, osteoinduction, human adipose-derived stem cells, mussel-inspired surface modification, bone tissue engineering, osteogenic differentiation, alkaline phosphatase, osteocalcin, Runx2, in vivo bone formation
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Adipose Tissue, Alkaline Phosphatase, Animals, Cell Adhesion, Cell Differentiation, Cells, Cultured, Humans, Immunohistochemistry, Male, Mice, Inbred BALB C, Mice, Nude, Osteogenesis, Peptide Fragments, Polyglycolic Acid, Stem Cells, Osteocalcin, Lactic Acid, Reverse Transcriptase Polymerase Chain Reaction, Models, Animal, Tissue Engineering, Cell Proliferation, Core Binding Factor Alpha 1 Subunit, Tissue Scaffolds, Bone Morphogenetic Protein 7

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