General functionalization route for cell adhesion on non-wetting surfaces
Abstract
We present a versatile route for promoting cell adhesion and viability on various non-wetting surfaces, inspired by mussel adhesion mechanism. The oxidative polymerization of dopamine, a small designer molecule of the DOPA-K motif found in mussels, results in the formation of a poly(dopamine) ad-layer on any material surface. We found that the poly(dopamine) coating can promote cell adhesion on any type of material surfaces including the well-known anti-adhesive substrate, poly(tetrafluoroethylene). According to our results, mammalian cells well adhered and underwent general cell adhesion processes (i.e., attachment to substrate, spreading, and cytoskeleton development) on poly(dopamine)-modified surfaces, while they barely adhered and spread on unmodified non-wetting surfaces. The mussel-inspired surface functionalization strategy is extremely useful because it does not require the time-consuming synthesis of complex linkers and the process is solvent-free and non-toxic. Therefore, it can be a powerful route for converting a variety of bioinert substrates into bioactive ones.
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The pHAF coating can modify a non-bioactive surface into a bioactive one.
“Other reports emphasized that the pHAF coating can modify a non-bioactive surface into a bioactive one [27].”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [27]
As a precursor of PDA, DA has functional groups (catechol and phenethylamine) and adhesive mechanism similar to DOPA, which offers DA the potential as a versatile surface modifier in various biomedical applications.
“As a precursor of PDA, DA has functional groups (catechol and phenethylamine) and adhesive mechanism similar to DOPA, which offers DA the potential as a versatile surface modifier in various biomedical applications [21–23].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [22]
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Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
Ryu, J., Ku, S. H., Lee, H., & Park, C. B. (2010) · Advanced Functional Materials · doi:10.1002/adfm.200902347 · Cited by 778 · see citing works
Self-citationPoly (lactic acid)-based biomaterials for orthopaedic regenerative engineering
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“[22] S.”
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“Other reports emphasized that the pHAF coating can modify a non-bioactive surface into a bioactive one [27].”
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Effects of polydopamine coating on the bioactivity of titanium for dental implants
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- Authors
- Ku, S. H., Ryu, J., Hong, S. K., Lee, H., & Park, C. B.
- Affiliations
- Korea Advanced Institute of Science and Technology (KR)
- Corresponding author
- Lee, Haeshin
- Park, Chan Beum
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 31
- Issue
- 9
- Pages
- 2535-2541
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1996833161
- Cited by
- 687
- References
- 1
- Citations by year
- 2026: 10 · 2025: 21 · 2024: 33 · 2023: 32 · 2022: 34 · 2021: 48 · 2020: 62 · 2019: 54 · 2018: 67 · 2017: 61 · 2016: 51 · 2015: 50 · 2014: 48 · 2013: 43 · 2012: 41
- FWCI
- 18.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- cell adhesion, non-wetting surfaces, polydopamine, mussel-inspired adhesion, polytetrafluoroethylene, anti-adhesive surfaces, surface functionalization, cell spreading, bioactive surfaces
- MeSH terms
- Animals, Cell Adhesion, Cell Line, Cell Survival, Dopamine, Osteoblasts, PC12 Cells, Wettability, Cell Shape, Mice, Rats, Photoelectron Spectroscopy
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