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Cited by 77Plant tissue culture and regenerationGenetic diversity and population structureGenetic Mapping and Diversity in Plants and Animals

Genetic Homogeneity Revealed Using SCoT, ISSR and RAPD Markers in Micropropagated Pittosporum eriocarpum Royle- An Endemic and Endangered Medicinal Plant

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Thakur, Julie, Dwivedi, Mayank D., Sourabh, Pragya, Uniyal, Prem L., Pandey, Arun K.·2016·PLOS ONE
DOI 10.1371/journal.pone.0159050PMC PMC4951010
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Abstract

Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0.4mg/l in combination IBA 0.6mg/l. In vitro regenerated shoots were rooted in MS medium supplemented with three auxins, of which 0.6 mg/l indole butyric acid proved to be the best for rooting (90%) with maximum number of roots per shoot. Thereafter, rooted plants were hardened and nearly 73% of rooted shoots were successfully acclimatized and established in the field. Start codon targeted (SCoT), inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) markers were used to validate the genetic homogeneity amongst nine in vitro raised plantlets with mother plant. DNA fingerprints of in vitro regenerated plantlets displayed monomorphic bands similar to mother plant, indicating homogeneity among the micropropagated plants with donor mother plant. The similarity values were calculated based on SCoT, ISSR and RAPD profiles which ranged from 0.89 to 1.00, 0.91 to 1.00 and 0.95 to 1.00 respectively. The dendrograms generated through Unweighted Pair Group Method with arithmetic mean (UPGMA) analysis revealed 97% similarity amongst micropropagated plants with donor mother plant, thus confirming genetic homogeneity of micropropagated clones. This is the first report on micropropagation and genetic homogeneity assessment of P. eriocarpum. The protocol would be useful for the conservation and large scale production of P. eriocarpum to meet the demand for medicinal formulations and also for the re-introduction of in vitro grown plants in the suitable natural habitats to restore the populations.

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  • In studies using a combination of two or three types of markers (SCoT, ISSR, RAPD), research revealed genetic fidelity of regenerated plantlets, even for those that had passed through the callus stage.

    “There are a number of studies in which the somaclonal variation analysis is carried out using a combination of two or three types of markers (Table 2), such as SCot, ISSR, and RAPD, and, in all cases, the research revealed the genetic fidelity of the regenerated plantlets (even for those that had passed through the callus stage) [73,74,75,76,77,78].”

    From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [78]

    The Genetic homogeneity assessment and Data analysis sections report testing regenerated Pittosporum plantlets with SCoT, ISSR and RAPD markers and finding 97% similarity to the mother plant. But Data analysis also notes a slight DNA-level change in one plant, and the Shoot induction and multiplication section describes basal callus formation without establishing that the tested plantlets regenerated through a callus stage. The source therefore supports this as a marker-based homogeneity study, but not the unqualified fidelity or callus-stage portions of the claim. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Thakur, J., Dwivedi, M. D., Sourabh, P., Uniyal, P. L., & Pandey, A. K.
Affiliations
University of Delhi (IN)
Corresponding author
Pandey, Arun K.
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2016-07-19
Volume
11
Issue
7
Pages
e0159050
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0159050
PMC
PMC4951010
OpenAlex
W2492139109
Cited by
77
References
0
Citations by year
2026: 4 · 2025: 6 · 2024: 4 · 2023: 11 · 2022: 12 · 2021: 15 · 2020: 13 · 2019: 7 · 2018: 2 · 2017: 3
FWCI
2.08
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
Department of Biotechnology, Ministry of Science and Technology (BT/Env/BS/01/2010/23.3.2012)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
Keywords
micropropagation, genetic homogeneity, in vitro regeneration, shoot rooting, acclimatization, SCoT markers, ISSR markers, RAPD markers, DNA fingerprinting, medicinal plants, threatened plant species, plant conservation
MeSH terms
Indoleacetic Acids, Plants, Medicinal, Seeds, DNA Fingerprinting, Codon, Initiator, Microsatellite Repeats, Random Amplified Polymorphic DNA Technique, Rosales, Endangered Species

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