Acclimatization of tissue cultured plantlets: from laboratory to land
Abstract
The ultimate success of micropropagation on a commercial scale depends on the ability to transfer plants out of culture on a large scale, at low cost and with high survival rates. During field transfer the in vitro grown plantlets are unable to compete with soil microbes and to cope with the environmental conditions. The in vitro culture conditions result in the plantlets with altered morphology, anatomy and physiology. In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, efforts are focused on the control of both physical and chemical environment and biohardening of micropropagated plantlets. This review describes the abiotic and biotic stresses and current developing methods for the acclimatization of microshoots.
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When producing Persian walnut in vitro explants, it is crucial to undergo hardening and acclimatization processes due to dysfunctional stomata of walnut in vitro-plantlets, resulting in excessive transpiration and heightened sensitivity to environments with increased evaporative demands and solar radiation.
“When producing Persian walnut in vitro explants, it is crucial to undergo hardening and acclimatization processes. This is due to the dysfunctional stomata of walnut in vitro-plantlets, resulting in excessive transpiration and heightened sensitivity to environments with increased evaporative demands and solar radiation (Chandra et al., 2010; Rathore et al., 2013; Manokari and Shekhawat, 2017).”
From Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra (2023) · cites this paper as Chandra et al., 2010
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Skała, E., Grąbkowska, R., Sitarek, P., Kuźma, Ł., Błauż, A., & Wysokińska, H. (2015) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-015-0816-1 · Cited by 38 · see citing works
Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets
Mohammed, M., Munir, M., & Ghazzawy, H. S. (2022) · Agronomy · doi:10.3390/agronomy13010078 · Cited by 35 · see citing works
Saeedi, S. A., Vahdati, K., Sarikhani, S., Daylami, S. D., Davarzani, M., Gruda, N. S., & Aliniaeifard, S. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1292045 · Cited by 30 · see citing works
Monica, H., & Kumaria, S. (2024) · Vegetos · doi:10.1007/s42535-024-01092-y · Cited by 6 · see citing works
Somaclonal variations induced by benzylaminopurine to enhance the fruit morphology of horn banana
SIANIPAR, N. F. (2024) · SABRAO Journal of Breeding and Genetics · doi:10.54910/sabrao2024.56.5.27 · Cited by 3 · see citing works
- Authors
- Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R.
- Affiliations
- Birla Institute of Technology, Mesra (IN)
- Corresponding author
- Chandra, Sheela
- Venue
- Biotechnology Letters
- Published
- 2010-05-09
- Volume
- 32
- Issue
- 9
- Pages
- 1199-1205
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2144702201
- Cited by
- 346
- References
- 0
- Citations by year
- 2026: 26 · 2025: 26 · 2024: 27 · 2023: 34 · 2022: 37 · 2021: 40 · 2020: 42 · 2019: 19 · 2018: 14 · 2017: 15 · 2016: 18 · 2015: 15 · 2014: 14 · 2013: 9 · 2012: 7
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- acclimatization, micropropagation, tissue culture plantlets, abiotic stress, biotic stress, plantlet survival, microshoots, environmental stress tolerance
- MeSH terms
- Acclimatization, Stress, Physiological, Plant Physiological Phenomena, Tissue Culture Techniques, Plant Development
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