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Cited by 98Infant Nutrition and HealthMicrobial Metabolites in Food BiotechnologyProtein Hydrolysis and Bioactive Peptides

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Dietary 2’-Fucosyllactose Enhances Operant Conditioning and Long-Term Potentiation via Gut-Brain Communication through the Vagus Nerve in Rodents

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Vazquez, Enrique, Barranco, Alejandro, Ramirez, Maria, Gruart, Agnes, Delgado-Garcia, Jose M., Jimenez, Maria L., Buck, Rachael, Rueda, Ricardo·2016·PLOS ONE
DOI 10.1371/journal.pone.0166070PMC PMC5113009
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Abstract

2´-fucosyllactose (2´-FL) is an abundant human milk oligosaccharide (HMO) in human milk with diverse biological effects. We recently reported ingested 2´-FL stimulates central nervous system (CNS) function, such as hippocampal long term potentiation (LTP) and learning and memory in rats. Conceivably the effect of 2´-FL on CNS function may be via the gut-brain axis (GBA), specifically the vagus nerve, and L-fucose (Fuc) may play a role. This study had two aims: (1) determine if the effect of ingested 2´-FL on the modulation of CNS function is dependent on the integrity of the molecule; and (2) confirm if oral 2´-FL modified hippocampal LTP and associative learning related skills in rats submitted to bilateral subdiaphragmatic vagotomy. Results showed that 2´-FL but not Fuc enhanced LTP, and vagotomy inhibited the effects of oral 2´-FL on LTP and associative learning related paradigms. Taken together, the data show that dietary 2´-FL but not its Fuc moiety affects cognitive domains and improves learning and memory in rats. This effect is dependent on vagus nerve integrity, suggesting GBA plays a role in 2´-FL-mediated cognitive benefits.

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  • Subdiaphragmatic vagal deafferentation highlights the involvement of vagal afferents in anxiety-like and fear-related behavior.

    “In addition, subdiaphragmatic vagal deafferentation (Vazquez et al., 2016) a method that eliminates abdominal vagal afferents, but keeps most efferents intact (Norgren and Smith, 1994) highlights the involvement of vagal afferents in anxiety-like (elevated plus maze, open-field test, food neophobia) and fear-related behavior (auditory-cued fear conditioning) (Klarer et al.,”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Vazquez et al., 2016

    The Vazquez citation identifies the surgical method, not the later claim about anxiety-like and fear-related behavior. In Material and Methods (page unavailable), Vazquez et al. describe bilateral subdiaphragmatic vagotomy that transects the vagal trunks and branches, rather than selective vagal deafferentation. (LLM, conf=0.86)

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  • Vagotomy inhibits the effects of the prebiotic 2'-Fucosyllactose in associative learning paradigms and hippocampal LTP.

    “Moreover, vagotomy inhibited the effects of the prebiotic 2'-Fucosyllactose in associative learning related paradigms and hippocampal LTP (Vazquez et al., 2016).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Vazquez et al., 2016

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Metadata

Authors
Vazquez, E., Barranco, A., Ramirez, M., Gruart, A., Delgado-Garcia, J. M., Jimenez, M. L., Buck, R., & Rueda, R.
Affiliations
  • Universidad Pablo de Olavide (ES)
  • Abbott Nutrition (United States) (US)
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2016-11-16
Volume
11
Issue
11
Pages
e0166070
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0166070
PMC
PMC5113009
OpenAlex
W2552391811
Cited by
98
References
2
Citations by year
2026: 11 · 2025: 14 · 2024: 14 · 2023: 12 · 2022: 5 · 2021: 13 · 2020: 15 · 2019: 8 · 2018: 5 · 2017: 1
FWCI
4.16
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Abbott Nutrition
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
Keywords
2'-fucosyllactose, human milk oligosaccharides, gut-brain axis, vagus nerve, long-term potentiation, hippocampus, operant conditioning, associative learning, memory, vagotomy, L-fucose, dietary oligosaccharides, cognitive function
MeSH terms
Administration, Oral, Animals, Brain, Conditioning, Operant, Diet, Digestive System, Fucose, Male, Trisaccharides, Vagotomy, Vagus Nerve, Rats, Sprague-Dawley, Long-Term Potentiation

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