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Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea
Open accessAbstract
Early-life microbial exposure is of particular importance to growth, immune system development and long-lasting health. Hence, early microbiota composition is a promising predictive biomarker for health and disease but still remains poorly characterized in regards to susceptibility to diarrhoea. In the present study, we aimed to assess if gut bacterial community diversity and composition during the suckling period were associated with differences in susceptibility of pigs to post-weaning diarrhoea. Twenty piglets from 5 sows (4 piglets / litter) were weaned in poor housing conditions to challenge their susceptibility to post-weaning diarrhoea. Two weeks after weaning, 13 pigs exhibited liquid faeces during 2 or 3 days and were defined as diarrhoeic (D) pigs. The other 7 pigs did not have diarrhea during the whole post-weaning experimental periodand were defined as healthy (H) pigs. Using a molecular characterisation of fecal microbiota with CE-SSCP fingerprint, Next Generation Sequencing and qPCR, we show that D and H pigs were mainly discriminated as early as postnatal day (PND) 7, i.e. 4 weeks before post-weaning diarrhoea occurence. At PND 7 H pigs displayed a lower evenness and a higher abundance of Prevotellaceae, Lachnospiraceae, Ruminocacaceae and Lactobacillaceae compared to D pigs. The sPLS regression method indicates that these bacterial families were strongly correlated to a higher Bacteroidetes abundance observed in PND 30 H pigs one week before diarrhoea. These results emphasize the potential of early microbiota diversity and composition as being an indicator of susceptibility to post-weaning diarrhoea. Furthermore, they support the health promoting strategies of pig herds through gut microbiota engineering.
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Gastrointestinal tract (gut) health in the young pig
Pluske, J. R., Turpin, D. L., & Kim, J.-C. (2018) · Animal Nutrition · doi:10.1016/j.aninu.2017.12.004 · Cited by 417
Piglet gut microbial shifts early in life: causes and effects
Guevarra, R. B., Lee, J. H., Lee, S. H., Seok, M.-J., Kim, D. W., Kang, B. N., Johnson, T. J., Isaacson, R. E., & Kim, H. B. (2019) · Journal of Animal Science and Biotechnology · doi:10.1186/s40104-018-0308-3 · Cited by 398
Ikeda-Ohtsubo, W., Brugman, S., Warden, C. H., Rebel, J. M. J., Folkerts, G., & Pieterse, C. M. J. (2018) · Frontiers in Nutrition · doi:10.3389/fnut.2018.00090 · Cited by 115
Metadata
- Authors
- Dou, S., Gadonna-Widehem, P., Rome, V., Hamoudi, D., Rhazi, L., Lakhal, L., Larcher, T., Bahi-Jaber, N., Pinon-Quintana, A., Guyonvarch, A., Huërou-Luron, I. L. E., & Abdennebi-Najar, L.
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2017-01-10
- Volume
- 12
- Issue
- 1
- Pages
- e0169851
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2569773245
- Cited by
- 264
- References
- 50
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- gold
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- Dou, S., Gadonna-Widehem, P., Rome, V., Hamoudi, D., Rhazi, L., Lakhal, L., Larcher, T., Bahi-Jaber, N., Pinon-Quintana, A., Guyonvarch, A., Huërou-Luron, I. L. E., & Abdennebi-Najar, L. (2017). Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea. PLOS ONE. https://doi.org/10.1371/journal.pone.0169851
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