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Screening human cell lines for viral infections applying RNA-Seq data analysis

Uphoff, Cord C., Pommerenke, Claudia, Denkmann, Sabine A., Drexler, Hans G.·2019·PLOS ONE
DOI 10.1371/journal.pone.0210404PMC PMC6328144
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  • A number of human cell lines appear to be infected with viruses, including human pathogens such as HTLV-2.

    “A number of human cell lines appear to be infected with viruses, including human pathogens such as HTLV-2 (Uphoff et al., 2019).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Uphoff et al., 2019

    The Results and discussion (page unavailable) identify cell lines with PCR-confirmed viral infections, supporting the general claim. But the two cell lines with HTLV-2-specific reads had only one and 16 reads; the paper treats similarly low-level viral reads as possible processing contamination, not evidence that those cell lines were infected with HTLV-2. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Uphoff, C. C., Pommerenke, C., Denkmann, S. A., & Drexler, H. G.
ORCID
Uphoff, Cord C.
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2019-01-10
Volume
14
Issue
1
Pages
e0210404
ISSN
1932-6203
DOI
10.1371/journal.pone.0210404
PMC
PMC6328144
Cited by
24
References
3
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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