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Good Cell Culture Practice for stem cells and stem-cell-derived models

Pamies, David·2016·ALTEX
DOI 10.14573/altex.1607121
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  • Protocol optimization has successfully reduced or eliminated fetal bovine serum (FBS) in line with GCCP recommendations.

    “Protocol optimization has successfully reduced or eliminated fetal bovine serum (FBS) in line with Good Cell Culture Practice (GCCP) recommendations (Coecke et al., 2005; Eskes et al., 2017; Pamies et al., 2017; Pamies et al., 2018; Tigges et al., 2021; Pamies et al., 2022)”

    From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Pamies et al., 2017

    The 'Cell purity, stability and functional integrity' section (page unavailable) reports a shift from serum-containing media toward serum-free and chemically defined media, consistent with reducing or eliminating FBS. The 'Medium conditions' section (page unavailable) says serum is still used in some stem-cell differentiation protocols. Neither passage establishes that protocol optimization successfully achieved the reduction or elimination claimed. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Comprehensive training for research groups within a multinational project consortium is of significant value to increase robustness and reproducibility of methods and to strictly adhere to agreed protocols or SOPs to facilitate/enable comparisons of research findings within the consortium.

    “Specifically, comprehensive training for research groups within a multinational project consortium is of significant value to increase robustness and reproducibility of methods and to strictly adhere to agreed protocols or SOPs to facilitate/enable comparisons of research findings within the consortium (Pamies et al., 2017).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Pamies et al., 2017

  • GCCP 2.0 is trying to complement these for stem cell-derived models and microphysiological systems (MPS).

    “GCCP 2.0 is trying to complement these for stem cell-derived models and microphysiological systems (MPS).”

    From Toward good in vitro reporting standards (2019) · cites this paper as Pamies et al., 2017a

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Authors
Pamies, D.
Venue
ALTEX
Publisher
ALTEX Edition
Published
2016
ISSN
1868-596X
DOI
10.14573/altex.1607121
Cited by
28
References
12

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