The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis
Open accessAbstract
Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacteria. Genes, however, make up only a part of the complex genomes of important bacterial pathogens. Here, we use an unbiased analysis to comprehensively identify genomic regions, including genes, domains, and intergenic elements, required for the optimal growth of Mycobacterium tuberculosis, a major global health pathogen. We found that several proteins jointly contain both domains required for optimal growth and domains that are dispensable. In addition, many non-coding regions, including regulatory elements and non-coding RNAs, are critical for mycobacterial growth. Our analysis shows that the genetic requirements for growth are more complex than can be appreciated using gene-centric analysis.
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Cites
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Cole, S. T., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S. V., Eiglmeier, K., Gas, S., Barry, C. E., et al. (1998) · Nature · doi:10.1038/31159 · Cited by 7,847
Genes required for mycobacterial growth defined by high density mutagenesis
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03425.x · Cited by 2,544
Self-citationGriffin, J. E., Gawronski, J. D., DeJesus, M. A., Ioerger, T. R., Akerley, B. J., & Sassetti, C. M. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002251 · Cited by 1,048
Self-citation
Cited by
DeJesus, M. A., Gerrick, E. R., Xu, W., Park, S. W., Long, J. E., Boutte, C. C., Rubin, E. J., Schnappinger, D., Ehrt, S., Fortune, S. M., et al. (2017) · mBio · doi:10.1128/mbio.02133-16 · Cited by 668
Self-citationRock, J. M., Hopkins, F. F., Chavez, A., Diallo, M., Chase, M. R., Gerrick, E. R., Pritchard, J. R., Church, G. M., Rubin, E. J., Sassetti, C. M., et al. (2017) · Nature Microbiology · doi:10.1038/nmicrobiol.2016.274 · Cited by 538
Self-citationTransposon insertion sequencing: a new tool for systems-level analysis of microorganisms
van Opijnen, T., & Camilli, A. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro3033 · Cited by 494
Stucki, D., Brites, D., Jeljeli, L., Coscolla, M., Liu, Q., Trauner, A., Fenner, L., Rutaihwa, L., Borrell, S., Luo, T., et al. (2016) · Nature Genetics · doi:10.1038/ng.3704 · Cited by 423
Tryptophan Biosynthesis Protects Mycobacteria from CD4 T-Cell-Mediated Killing
Zhang, Y. J., Reddy, M. C., Ioerger, T. R., Rothchild, A. C., Dartois, V., Schuster, B. M., Trauner, A., Wallis, D., Galaviz, S., Huttenhower, C., et al. (2013) · Cell · doi:10.1016/j.cell.2013.10.045 · Cited by 351
“In order to define the set of Mtb genes required for surviving the CD4 T cell response, we infected both wild-type (WT) and MHC class II KO (MHCII / ) mice with a library of Mtb transposon mutants (Zhang et al., 2012).”
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Metadata
- Authors
- Zhang, Y. J., Ioerger, T. R., Huttenhower, C., Long, J. E., Sassetti, C. M., Sacchettini, J. C., & Rubin, E. J.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-09-27
- Volume
- 8
- Issue
- 9
- Pages
- e1002946
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- OpenAlex
- W2125301421
- Cited by
- 242
- References
- 3
- FWCI
- 16.59
- Type
- journal article
- Language
- en
- Update notices
- correction (2013-07-25)
- Open access
- gold
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- Zhang, Y. J., Ioerger, T. R., Huttenhower, C., Long, J. E., Sassetti, C. M., Sacchettini, J. C., & Rubin, E. J. (2012). Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1002946
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
