Regulation and expression of elrD1 and elrD2 transcripts during early Xenopus laevis development
Open accessAbstract
The Xenopus laevis elrD (elav-like ribonucleoprotein D) gene is a member of the elav/Hu family which encodes RNA-binding proteins. Most of the elav/Hu genes are expressed in the nervous system, where they are implicated in the development and maintenance of neurons. The regulation of elrD gene expression involves two promoters, pD1 and pD2. In this study, we analyzed the neural specificity directed by both promoters. They were fused to the gene encoding green fluorescent protein, and their ability to drive neural expression in injected Xenopus embryos was examined. We show that both promoters direct neural expression and that whole promoter sequences are needed to induce neural specific expression. Finally, we analyzed the spatial and temporal localization of the two elrD transcripts, elrD1 and elrD2. We found that the two transcripts present the same tissue-specific pattern of expression, with distinct developmental regulation. Our results show a complex regulation of the elrD gene and suggest that different transcripts resulting from alternative splicing of the elrD gene probably define different neurons.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
These variants encode transcripts exhibiting unique temporal expression during Xenopus nervous system development.
“These variants encode transcripts exhibiting unique temporal expression during Xenopus nervous system development (Nassar 2011).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Nassar 2011
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A conserved family of elav-like genes in vertebrates.
Good, P. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.10.4557 · Cited by 319 · see citing works
Neuronal ELAV proteins enhance mRNA stability by a PKCα-dependent pathway
Pascale, A., Amadio, M., Scapagnini, G., Lanni, C., Racchi, M., Provenzani, A., Govoni, S., Alkon, D. L., & Quattrone, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504702102 · Cited by 129 · see citing works
A role for the ELAV RNA-binding proteins in neural stem cells: stabilization of<i>Msi1</i>mRNA
Ratti, A., Fallini, C., Cova, L., Fantozzi, R., Calzarossa, C., Zennaro, E., Pascale, A., Quattrone, A., & Silani, V. (2006) · Journal of Cell Science · doi:10.1242/jcs.02852 · Cited by 86 · see citing works
Samson, M.-L. (1998) · Genetics · doi:10.1093/genetics/150.2.723 · Cited by 51 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Richard K.T. Kam, Yonglong Chen, Sun-On Chan, Wood-Yee Chan, Igor B. Dawid and Hui Zhao Int. J. Dev. Biol. (2010) 54: 1355-1360 (doi: 10.1387/ijdb.092984rk) Differential expression of the Brunol/CELF family genes during Xenopus laevis early development · DOI
Cited by
Sorted by citations
Bronicki, L. M., & Jasmin, B. J. (2013) · RNA · doi:10.1261/rna.039164.113 · Cited by 126 · see citing works
- Authors
- Nassar, F.
- Affiliations
- Université Paris-Sud (FR)
- Corresponding author
- Nassar, Fida
- Venue
- The International Journal of Developmental Biology
- Publisher
- EHU Press
- Published
- 2011
- Volume
- 55
- Issue
- 1
- Pages
- 127-132
- ISSN
- 0214-6282 (ISSN-L)
- OpenAlex
- W2041760011
- Cited by
- 1
- References
- 4
- Citations by year
- 2013: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The International Journal of Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Association pour la Recherche sur le Cancer
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- RNA-binding proteins, Xenopus laevis, alternative splicing, promoter analysis, neuronal gene expression, developmental gene regulation, transcript localization
- MeSH terms
- Animals, Brain, Embryo, Nonmammalian, Microscopy, Fluorescence, Promoter Regions, Genetic, Ribonucleoproteins, RNA, Messenger, Time Factors, Transcription, Genetic, Xenopus laevis, Alternative Splicing, In Situ Hybridization, Gene Expression Regulation, Developmental, Protein Isoforms, Xenopus Proteins, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
