Strategy to replace animal-derived ECM by a modular and highly defined matrix
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The increasing availability of recombinant and chemically defined reagents provides opportunities to reduce reliance on animal-derived materials and to improve the definition of cell culture systems used in toxicological testing.
“The increasing availability of recombinant and chemically defined reagents provides opportunities to reduce reliance on animal-derived materials and to improve the definition of cell culture systems used in toxicological testing (Nesterenko et al., 2020; Cassotta et al., 2022; Fraser et al., 2025).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Nesterenko et al., 2020
ZTFn supports pluripotency maintenance and neural crest differentiation.
“Similarly, ZTFn, a human protein based recombinant polymer modified with fibronectin domains, supports pluripotency maintenance and neural crest differentiation (Hill et al., 2019; Nesterenko et al., 2019; Nesterenko et al., 2020).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Nesterenko et al., 2020
ZTFn can substitute bovine serum-derived fibronectin as a coating for the UKN2 assay, yielding comparable bioactivity and sensitivity to model toxicants.
“In a proof-of-concept study, Nesterenko et al. (2020) demonstrated that ZTFn can substitute bovine serum-derived fibronectin as a coating for the UKN2 assay, yielding comparable bioactivity and sensitivity to model toxicants.”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Nesterenko et al. (2020)
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https://www.altex.org/index.php/altex/article/view/1339
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Cited by
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Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0)
Pamies, D. (2021) · ALTEX · doi:10.14573/altex.2111011 · Cited by 100 · see citing works
Spänle, J. V., Haiber, L. M., & Seeger, B. (2026) · Frontiers in Toxicology · doi:10.3389/ftox.2026.1800157 · Cited by 1 · see citing works
- Authors
- Nesterenko, Y.
- Venue
- ALTEX
- Publisher
- ALTEX Edition
- Published
- 2020
- ISSN
- 1868-596X
- Cited by
- 1
- References
- 1
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