Fetal Bovine Serum (FBS): past ‐ present ‐ future
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Fully defined formulations remain challenging to implement for many systems.
“However, fully defined formulations remain challenging to implement for many systems (Galbraith et al., 2018; Ritacco et al., 2018; van der Valk et al., 2018).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as van der Valk et al., 2018
ESAC recommends avoiding FBS use unless scientifically justified.
“Regulatory frameworks already provide clear guidance in his direction: the OECD Good Ín Vitro Method Practices (GIVIMP) guidelines recommend minimizing or eliminating undefined components such as FBS (OECD, 2018), while the Centre for the Validation of Alternative Methods Scientific Advisory Committee (ESAC) recommends avoiding FBS use unless scientifically justified (van der Valk et al., 2018).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as van der Valk et al., 2018
All animal-derived materials and reagents are obtained from different animal groups and result in batch-to-batch variation, which may lead to reliability and integrity concerns for generated in vitro data.
“Notably, all animal‐derived materials and reagents are obtained from different animal groups and result in batch‐to‐batch variation, which may lead to reliability and integrity concerns for generated in vitro data [18, 19].”
From A worldwide survey on the use of animal‐derived materials and reagents in scientific experimentation (2022) · cites this paper as [19]
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