Signaling responses to high and moderate load strength exercise in trained muscle
Open accessAbstract
We examined signaling responses in the skeletal muscle of strength athletes after strength exercises under high and moderate load. Eight trained male powerlifters were recruited. The volunteers performed four sets of leg presses to volitional fatigue using a moderate load (65% 1-repetition maximum [1RM]) for one leg, and a high load (85% 1RM) for the contralateral leg. The work volume performed by the leg moving a moderate load was higher than that of the contralateral leg moving a high load. Biopsy of the m. vastus lateralis was performed before, and at 1, 5, and 10 h after, cessation of exercise. Phosphorylation of p70S6kThr389, 4E-BP1Thr37/46, and ACCSer79 increased after moderate load exercises, whereas phosphorylation of ERK1/2Thr202/Tyr204 increased, and that of eEF2Thr56 decreased, after high load exercises. Exercise under a moderate load and a high work volume activated mTORC1-dependent signaling in trained skeletal muscle, whereas exercise under a high load but lower work volume activated the MEK-ERK1/2 signaling cascade and eEF2.
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Other research demonstrates divergent responses in intracellular anabolic signaling and myogenic gene expression when training in moderate- (74% to 85% 1RM) and lower (54% to 65% 1RM) loading zones, with selective activation of different kinase pathways between conditions.
“Other research demonstrates divergent responses in intracellular anabolic signaling and myogenic gene expression when training in moderate- (from 74% to 85% 1RM) and lower (from 54% to 65% 1RM) loading zones, with selective activation of different kinase pathways observed between conditions [40,41].”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [41]
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Experimental — accuracy not yet measuredAcute and longitudinal data suggest a potential hypertrophic benefit to combining loading ranges as part of a structured RT program.
“Both acute [40,41] and longitudinal [83,84,85,86] data suggest a potential hypertrophic benefit to combining loading ranges as part of a structured RT program”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [41]
The Results and Conclusions report that high- and moderate-load exercise activated different signaling pathways in trained muscle, providing an acute mechanistic rationale for using both loading ranges. The study did not test a program combining those ranges or measure whether combining them improves hypertrophy; the longitudinal citations in the sentence are separate from this source. (LLM, conf=0.82)
Experimental — accuracy not yet measuredPreliminary evidence for potential differences in intracellular anabolic signaling between loading zones cannot be discounted.
“That said, preliminary evidence for potential differences in intracellular anabolic signaling between loading zones cannot be discounted [40,41], and may have practical implications for RT program design.”
From Loading recommendations for muscle strength, hypertrophy, and local endurance: a re-examination of the repetition continuum. (2021) · cites this paper as [41]
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- Authors
- Lysenko, E. A., Popov, D. V., Vepkhvadze, T. F., Sharova, A. P., & Vinogradova, O. L.
- Affiliations
- Russian Academy of Sciences (RU)
- Laboratory of Exercise Physiology SSC RF Institute of Biomedical Problems RAS Moscow Russia
- Corresponding author
- Lysenko, Evgeny A.
- Venue
- Physiological Reports
- Publisher
- Wiley
- Published
- 2019-05
- Volume
- 7
- Issue
- 9
- Pages
- e14100
- ISSN
- 2051-817X (ISSN-L)
- PMC
- PMC6517334
- OpenAlex
- W2945880639
- Cited by
- 12
- References
- 13
- Citations by year
- 2023: 2 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Physiological Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Russian Foundation for Basic Research (17‐04‐00878a)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Keywords
- resistance training, load intensity, work volume, mTORC1, MEK/ERK signaling, elongation factor 2, p70S6K, 4E-BP1, ACC, vastus lateralis, powerlifters, muscle fatigue
- MeSH terms
- Adult, Biopsy, Humans, Hydrocortisone, Leg, Male, Muscle Proteins, Phosphorylation, Testosterone, Signal Transduction, Exercise, Weight-Bearing, Muscle, Skeletal, Muscle Fatigue, Lactic Acid, Resistance Training, Young Adult, Athletes
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