Genomic insights into cyanobacterial protein translocation systems
Abstract
Cyanobacteria are ubiquitous oxygenic photosynthetic bacteria with a versatile metabolism that is highly dependent on effective protein targeting. Protein sorting in diderm bacteria is not trivial and, in cyanobacteria, even less so due to the presence of a complex membrane system: the outer membrane, the plasma membrane and the thylakoid membrane. In cyanobacteria, protein import into the thylakoids is essential for photosynthesis, export to the periplasm fulfills a multifunctional role in maintaining cell homeostasis, and secretion mediates motility, DNA uptake and environmental interactions. Intriguingly, only one set of genes for the general secretory and the twin-arginine translocation pathways seem to be present. However, these systems have to operate in both plasma and thylakoid membranes. This raises the question of how substrates are recognized and targeted to their correct, final destination. Additional complexities arise when a protein has to be secreted across the outer membrane, where very little is known regarding the mechanisms involved. Given their ecological importance and biotechnological interest, a better understanding of protein targeting in cyanobacteria is of great value. This review will provide insights into the known knowns of protein targeting, propose hypotheses based on available genomic sequences and discuss future directions.
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Sec generally transports unfolded proteins, whereas Tat can transport folded proteins, making pathway selection important for enzyme activity and stability.
“Sec generally transports unfolded proteins, whereas Tat can transport folded proteins, making pathway selection important for enzyme activity and stability [167].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [167]
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Function and benefits of natural competence in cyanobacteria: from ecology to targeted manipulation
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Self-citationHanamghar, S. S., Mellor, S. B., Mikkelsen, L., Crocoll, C., Motawie, M. S., Russo, D. A., Jensen, P. E., & Zedler, J. A. Z. (2025) · ACS Synthetic Biology · doi:10.1021/acssynbio.4c00800 · Cited by 9 · see citing works
Self-citationWalshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1 · see citing works
“Sec-and Tat-dependent translocation pathways are particularly relevant for targeting proteins to the periplasm or extracellular space; Sec generally transports unfolded proteins, whereas Tat can transport folded proteins, making pathway selection important for enzyme activity and stability [167].”
- Authors
- Russo, D. A., & Zedler, J. A. Z.
- Affiliations
- Friedrich Schiller University Jena (DE)
- Corresponding author
- Zedler, Julie A. Z.
- Venue
- Biological Chemistry
- Publisher
- Walter de Gruyter GmbH
- Published
- 2020-10-12
- Volume
- 402
- Issue
- 1
- Pages
- 39-54
- ISSN
- 1431-6730 (ISSN-L)
- 1437-4315
- OpenAlex
- W3091917754
- Cited by
- 25
- References
- 121
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 3
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biological Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- cyanobacteria, protein translocation, protein targeting, twin-arginine translocation, general secretory pathway, thylakoid membranes, outer membrane, periplasmic export, protein secretion, photosynthesis, membrane protein sorting, genome analysis
- MeSH terms
- Protein Translocation Systems, Cyanobacteria, Bacterial Proteins, Biological Transport
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