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Über die Extraktion von Bakterien mit Phenol/Wasser

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Westphal, Otto, Lüderitz, Otto, Bister, Fritz·1952·Zeitschrift für Naturforschung B
DOI 10.1515/znb-1952-0303
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Abstract

Es werden zwei einfache Verfahren zur Extraktion von Bakterien mit Phenol/Wasser angegeben. Nach Behandlung von gramnegativen Bakterien mit Phenol/Wasser-Emulsionen in der Kälte während weniger Minuten erhält man in der wäßrigen Phase die somatischen Glykoproteide der Bakterien (hauptsächlich 0-Antigene) in praktisch quantitativer Ausbeute. Nach Extraktion der Bakterien mit erwärmten, homogenen Phenol/Wasser-Mischungen und Trennen der Phasen in der Kälte finden sich in der wäßrigen Phase die proteinfreien Polysaccharide neben Nucleinsäuren. Einige chemische und immunologische Eigenschaften der nach den beiden Verfahren dargestellten Glykoproteide und Polysaccharide werden beschrieben.

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Metadata

Authors
Westphal, O., Lüderitz, O., & Bister, F.
Venue
Zeitschrift für Naturforschung B
Publisher
Walter de Gruyter GmbH
Published
1952-03-01
Volume
7
Issue
3
Pages
148-155
ISSN
  • 0044-3174 (ISSN-L)
  • 1865-7117
  • 0932-0776
DOI
10.1515/znb-1952-0303
OpenAlex
W1989961245
Cited by
1,853
References
0
FWCI
19.29
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Über die Extraktion von Bakterien mit Phenol/Wasser. https://app.cochat.ai/papers/10.1515/znb-1952-0303
The paper
Westphal, O., Lüderitz, O., & Bister, F. (1952). Über die Extraktion von Bakterien mit Phenol/Wasser. Zeitschrift für Naturforschung B. https://doi.org/10.1515/znb-1952-0303

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