The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
MAP2 and tau segregate into dendritic and axonal domains after the elaboration of morphologically distinct neurites: an immunocytochemical study of cultured rat cerebrum
Open accessAbstract
We sought to determine whether the strict segregation of MAP2 and tau into somatodendritic and axonal compartments in situ was maintained in dissociated neuronal cultures of the rat cerebrum. Cultures grown under serum-free conditions were immunolabeled with monoclonal antibodies specific for MAP2 and tau. At 14 d after plating, a clear distinction between MAP2- and tau-immunoreactive neurites was apparent. MAP2- immunoreactive neurites were relatively short, thick, tapering, and branched. Tau-immunoreactive neurites formed a crisscrossing meshwork of long, fine-caliber neurites, which, in more densely plated cultures, had a tendency to form thick, ropelike fascicles. Unlike the MAP2 pattern, tau antibodies labeled somata only lightly. Since distinct populations of neurites were labeled with the 2 antibodies, we sought to observe the development of the topographically distinct compartments by double-labeled immunocytochemistry with both polyclonal and monoclonal antibodies to MAP2 and tau. Cells observed within the first 8 hr after plating demonstrated equally intense MAP2 and tau immunoreactivity in a coextensive distribution throughout the cell body and initial neurites. By 16 hr, some neurites began to assume dendritic and axonal features; however, many such processes contained reaction product for both MAP2 and tau. Beginning at this time, neurites that appeared axonal showed a progressively weaker reaction with MAP2 antibodies, and neurites that appeared dendritic showed a progressively weaker reaction with tau antibodies. In most neurites the diminution appeared to occur uniformly over the entire extent of the neurite. During this transformation period there were occasional axon-like neurites that contained MAP2 immunoreactivity proximally, while tau immunoreactivity extended over the entire length of the neurite. We conclude that neurons in culture are able to compartmentalize MAP2 and tau into their appropriate processes and only attain an apparently homogeneous population of one of these MAPs after the neuron has assumed dendritic and axonal features. The analysis also lends indirect support to the hypothesis that microtubule-associated proteins (MAPs) form this association at the distal extent of the growing neurite.
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“contained MAP2 (Supplementary video 4), a marker for mature dendrites38.”
From Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB (2021) · cites this paper as [38]
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Cites
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“The work of Caceres et al. (1986) with rat hippocampal cultures describes the progressive loss of MAP2 immunoreactivity in a proximodistal fashion during in vitro development of an axon.”
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Amyloid oligomers exacerbate tau pathology in a mouse model of tauopathy
Selenica, M.-L. B., Brownlow, M., Jimenez, J. P., Lee, D. C., Pena, G., Dickey, C. A., Gordon, M. N., & Morgan, D. (2013) · Neurodegenerative Diseases · doi:10.1159/000337230 · Cited by 10,359
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Buée, L., Bussière, T., Buée‐Scherrer, V., Delacourte, A., & Hof, P. R. (2000) · Brain Research Reviews · doi:10.1016/s0165-0173(00)00019-9 · Cited by 2,142
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Inhibition of neurite polarity by tau antisense oligonucleotides in primary cerebellar neurons
Cáceres, A., & Kosik, K. S. (1990) · Nature · doi:10.1038/343461a0 · Cited by 656
Self-citation
Metadata
- Authors
- Kosik, K., & Finch, E.
- Affiliations
- Brigham and Women's Hospital (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1987-10-01
- Volume
- 7
- Issue
- 10
- Pages
- 3142-3153
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6569170
- OpenAlex
- W1812094318
- Cited by
- 368
- References
- 28
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 7 · 2022: 14 · 2021: 12 · 2020: 6 · 2019: 5 · 2018: 10 · 2017: 14 · 2016: 17 · 2015: 13 · 2014: 15 · 2013: 7 · 2012: 13
- FWCI
- 10.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ag006172-05, 5k07ns000835-03)
- National Institute on Aging (5 R01 AG06172)
- National Institute of Neurological Disorders and Stroke (5K07 NS00835)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Keywords
- MAP2, tau protein, microtubule-associated proteins, dendritic compartmentalization, neurite development, neurite fasciculation, cultured neurons, microtubule cytoskeleton
- MeSH terms
- Animals, Axons, Cells, Cultured, Cerebral Cortex, Dendrites, Immunohistochemistry, Microtubule-Associated Proteins, Neurons, Staining and Labeling, Time Factors, tau Proteins, Rats
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