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Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture
Open accessAbstract
We have previously shown that retinoic acid-treated cultures of the P19 line of embryonal carcinoma cells differentiate into neurons, glia, and fibroblast-like cells (Jones-Villeneuve et al., 1982). We report here that the monoclonal antibody HNK-1 reacts with the neurons at a very early stage of their differentiation and is, therefore, an early marker of the neuronal lineage. Cells in differentiated P 19 cultures synthesized acetylcholine but not catecholamines, suggesting that at least some of the neurons are cholinergic. The neurons also carry high- affinity uptake sites for GABA but not for serotonin. In long-term cultures, neuronal processes differentiated into axons and dendrites, which formed synapses. This biological system should prove valuable for examining the development and maturation of cholinergic neurons, since their differentiation occurs in cell culture.
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Citations
Cited by
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Mullen, R. J., Buck, C. R., & Smith, A. M. (1992) · Development · doi:10.1242/dev.116.1.201 · Cited by 2,351
Qi, Y., Wang, J. K. T., McMillian, M., & Chikaraishi, D. M. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-04-01217.1997 · Cited by 330
An activated Notch suppresses neurogenesis and myogenesis but not gliogenesis in mammalian cells
Nye, J. S., Kopan, R., & Axel, R. (1994) · Development · doi:10.1242/dev.120.9.2421 · Cited by 294
From embryonal carcinoma cells to neurons: The P19 pathway
Bain, G., Ray, W. J., Yao, M., & Gottlieb, D. I. (1994) · BioEssays · doi:10.1002/bies.950160509 · Cited by 240
Kasashima, K., Terashima, K., Yamamoto, K., Sakashita, E., & Sakamoto, H. (1999) · Genes to Cells · doi:10.1046/j.1365-2443.1999.00292.x · Cited by 120
Metadata
- Authors
- McBurney, M., Reuhl, K., Ally, A., Nasipuri, S., Bell, J., & Craig, J.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1988-03-01
- Volume
- 8
- Issue
- 3
- Pages
- 1063-1073
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- OpenAlex
- W1576171467
- Cited by
- 199
- References
- 0
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- McBurney, M., Reuhl, K., Ally, A., Nasipuri, S., Bell, J., & Craig, J. (1988). Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. The Journal of Neuroscience. https://doi.org/10.1523/jneurosci.08-03-01063.1988
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