Swelling-induced release of glutamate, aspartate, and taurine from astrocyte cultures
Open accessAbstract
Swelling of primary astrocyte cultures by exposing them to hypotonic media caused release of label after the cells had been allowed to accumulate 3H-L-glutamate, 3H-D-aspartate, or 3H-taurine. Comparable release of endogenous L-glutamate or taurine, as measured by high-pressure liquid chromatography (HPLC), was also found. Release of label was not affected by treating the cells with cytochalasin B, indicating that microfilament polymerization was not significantly involved. Hypotonic-induced release did not appear to principally involve reversal of the Na(+)-dependent uptake system since increasing external K+ to depolarize the cells by replacement of external Na+, thus maintaining isotonic conditions, increased release to a lesser extent. Threo beta-hydroxyaspartate, a potent 3H-L-glutamate uptake blocker, added externally stimulated efflux of 3H-L-glutamate independently of the swelling-induced efflux. Upon restoration of swollen cells to isotonic medium they showed an unimpaired ability to take up 3H-L-glutamate. The swelling-induced release of label was inhibited by a number of anion transport inhibitors, one of which has been shown to significantly improve outcome in an experimental brain trauma/hypoxia model in which astrocyte swelling is an early event.
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Cites
Brain Lesions, Obesity, and Other Disturbances in Mice Treated with Monosodium Glutamate
Olney, J. (1969) · Science · doi:10.1126/science.164.3880.719 · Cited by 1,885 · see citing works
Cell Damage in the Brain: A Speculative Synthesis
Siesjö, B. K. (1981) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.1981.18 · Cited by 1,807 · see citing works
Blockade of N -Methyl-D-Aspartate Receptors May Protect Against Ischemic Damage in the Brain
Simon, R. P., Swan, J. H., Griffiths, T., & Meldrum, B. S. (1984) · Science · doi:10.1126/science.6093256 · Cited by 1,682 · see citing works
The Role of Excitatory Amino Acids and NMDA Receptors in Traumatic Brain Injury
Faden, A. I., Demediuk, P., Panter, S. S., & Vink, R. (1989) · Science · doi:10.1126/science.2567056 · Cited by 1,508 · see citing works
Glutamine Synthetase: Glial Localization in Brain
Martinez-Hernandez, A., Bell, K. P., & Norenberg, M. D. (1977) · Science · doi:10.1126/science.14400 · Cited by 1,183 · see citing works
Stretch‐activated single ion channel currents in tissue‐cultured embryonic chick skeletal muscle.
Guharay, F., & Sachs, F. (1984) · The Journal of Physiology · doi:10.1113/jphysiol.1984.sp015317 · Cited by 997 · see citing works
Current topics in membranes and transport
Silano, V. (1976) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(76)80018-9 · Cited by 851 · see citing works
Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers?
Lansman, J. B., Hallam, T. J., & RINK, T. J. (1987) · Nature · doi:10.1038/325811a0 · Cited by 699 · see citing works
Pressure-sensitive ion channel in Escherichia coli.
Martinac, B., Buechner, M., Delcour, A. H., Adler, J., & Kung, C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.8.2297 · Cited by 656 · see citing works
Hertz, L. (1979) · Progress in Neurobiology · doi:10.1016/0301-0082(79)90018-2 · Cited by 629 · see citing works
High performance liquid chromatographic determination of amino acids in the picomole range
Hill, D. W., Walters, F. H., Wilson, T. D., et al. (1979) · Analytical Chemistry · doi:10.1021/ac50044a055 · Cited by 505 · see citing works
Responses of lymphocytes to anisotonic media: volume-regulating behavior
Grinstein, S., Rothstein, A., Sarkadi, B., & Gelfand, E. W. (1984) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1984.246.3.c204 · Cited by 427 · see citing works
Mediation of cell volume regulation by Ca2+ influx through stretch-activated channels
Christensen, O. (1987) · Nature · doi:10.1038/330066a0 · Cited by 426 · see citing works
A Mechanosensitive Ion Channel in the Yeast Plasma Membrane
Gustin, M. C., Zhou, X., Martinac, B., & Kung, C. (1988) · Science · doi:10.1126/science.2460920 · Cited by 374 · see citing works
AMINO ACID AND PROTEIN METABOLISM‐VI CEREBRAL COMPARTMENTS OF GLUTAMIC ACID METABOLISM
Berl, S., Lajtha, Á., & Waelsch, H. (1961) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1961.tb13503.x · Cited by 297 · see citing works
Volume regulation in astrocytes: A role for taurine as an osmoeffector
Morales, H. P., & Schousboe, A. (1988) · Journal of Neuroscience Research · doi:10.1002/jnr.490200415 · Cited by 261 · see citing works
Mechanical transduction in biological systems.
Sachs, F. (1988) · PubMed · Cited by 252 · see citing works
Textbook of General Physiology
Davson, H. (1937) · JAMA · doi:10.1001/jama.1937.02780520055037 · Cited by 243 · see citing works
Furosemide inhibition of chloride transport in human red blood cells.
Brazy, P. C., & Gunn, R. B. (1976) · The Journal of General Physiology · doi:10.1085/jgp.68.6.583 · Cited by 233 · see citing works
Function of astroglia in the water‐ion metabolism of the central nervous system
Gerschenfeld, H. M., Wald, F., Zadunaisky, J. A., et al. (1959) · Neurology · doi:10.1212/wnl.9.6.412 · Cited by 233 · see citing works
A Possible Role for Taurine in Osmoregulation Within the Brain
Wade, J. V., Olson, J. P., Samson, F. E., et al. (1988) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1988.tb01807.x · Cited by 225 · see citing works
Kinetic characteristics of the glutamate uptake into normal astrocytes in cultures
Hertz, L., Schousboe, A., Boechler, N., Mukerji, S., & FEDOROFF, S. (1978) · Neurochemical Research · doi:10.1007/bf00964356 · Cited by 214 · see citing works
PHYSIOLOGICAL AND PATHOLOGICAL ASPECTS OF ASTROCYTIC SWELLING
Kimelberg, H. K., & Ransom, B. R. (1986) · Elsevier eBooks · doi:10.1016/b978-0-12-250453-2.50009-8 · Cited by 192 · see citing works
Self-citationDoes taurine act as an osmoregulatory substance in the rat brain?
Solís, J. M., Herranz, A. S., Herreras, Ó., et al. (1988) · Neuroscience Letters · doi:10.1016/0304-3940(88)90248-0 · Cited by 188 · see citing works
Kimelberg, H. K., & Frangakis, M. V. (1985) · Brain Research · doi:10.1016/0006-8993(85)91282-x · Cited by 185 · see citing works
Self-citationAdaptation to chronic hypoosmolality in rats
Verbalis, J. G., & Drutarosky, M. D. (1988) · Kidney International · doi:10.1038/ki.1988.188 · Cited by 177 · see citing works
Dissociation of neonatal rat brain by dispase for preparation of primary astrocyte cultures
Frangakis, M. V., & Kimelberg, H. K. (1984) · Neurochemical Research · doi:10.1007/bf00968079 · Cited by 173 · see citing works
Self-citationExogenous Glutamate Is Metabolized to Glutamine and Exported by Rat Primary Astrocyte Cultures
Waniewski, R. A., & Martin, D. L. (1986) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1986.tb02863.x · Cited by 170 · see citing works
Self-citationThe Astrocyte in Liver Disease
Norenberg, M. D. (1981) · Advances in cellular neurobiology · doi:10.1016/b978-0-12-008302-2.50013-4 · Cited by 141 · see citing works
Cellular events during partial cerebral ischemia
García, J. H., Kalimo, H., Kamijyo, Y., & Trump, B. F. (1977) · Virchows Archiv B Cell Pathology Including Molecular Pathology · doi:10.1007/bf02889433 · Cited by 135 · see citing works
A TEXTBOOK OF GENERAL PHYSIOLOGY
Davson, H. (1965) · The American Journal of the Medical Sciences · doi:10.1097/00000441-196504000-00048 · Cited by 125 · see citing works
Activity of ion channels during volume regulation by clonal N1E115 neuroblastoma cells.
Falke, L. C., & Misler, S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.10.3919 · Cited by 116 · see citing works
Baroreceptor mechanisms at the cellular level.
Sachs, F. (1987) · PubMed · Cited by 109 · see citing works
Energy‐dependent volume regulation in primary cultured cerebral astrocytes
Olson, J. E., Sankar, R., Holtzman, D. H., James, A., & Fleischhacker, D. S. (1986) · Journal of Cellular Physiology · doi:10.1002/jcp.1041280211 · Cited by 107 · see citing works
Volume Regulation in Cultured Cells
Hoffmann, E. K. (1987) · Current topics in membranes and transport · doi:10.1016/s0070-2161(08)60370-6 · Cited by 104 · see citing works
Crayfish stretch receptor: an investigation with voltage‐clamp and ion‐sensitive electrodes.
Brown, H. M., Ottoson, D., & Rydqvist, B. (1978) · The Journal of Physiology · doi:10.1113/jphysiol.1978.sp012533 · Cited by 101 · see citing works
Kempski, O. S., Chaussy, L., Gross, U., Zimmer, M., & Baethmann, A. J. (1983) · Brain Research · doi:10.1016/0006-8993(83)90180-4 · Cited by 98 · see citing works
Volume Regulation in Cells of Euryhaline Invertebrates
Gilles, R. (1987) · Current topics in membranes and transport · doi:10.1016/s0070-2161(08)60372-x · Cited by 92 · see citing works
THE EFFECT OF ACUTE HYPOXIA AND HYPERCAPNIA ON THE ULTRASTRUCTURE OF THE CENTRAL NERVOUS SYSTEM
Bakay, L., & Lee, J. C. (1968) · Brain · doi:10.1093/brain/91.4.697 · Cited by 90 · see citing works
Thurston, J. H., Hauhart, R. E., & Nelson, J. S. (1987) · Metabolic Brain Disease · doi:10.1007/bf00999694 · Cited by 90 · see citing works
Philibert, R. A., Rogers, K. L., Allen, A. J., et al. (1988) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1988.tb04844.x · Cited by 81 · see citing works
Ultrastructural features of a brain injury model in cat
Barron, K. D., Dentinger, M. P., Kimelberg, H. K., Nelson, L. R., Bourke, R. S., Keegan, S., Mankes, R. F., & Cragoe, E. J. (1988) · Acta Neuropathologica · doi:10.1007/bf00690538 · Cited by 78 · see citing works
Self-citationVolume regulation of chinese hamster ovary cells in anisoosmotic media
Sarkadi, B., Attisano, L., Grinstein, S., et al. (1984) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(84)90287-6 · Cited by 67 · see citing works
Protein analysis of mammalian cells in monolayer culture using the bicinchoninic assay
Goldschmidt, R. C., & Kimelberg, H. K. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90010-9 · Cited by 62 · see citing works
Self-citationCragoe, E. J., Woltersdorf, O. W., GOULD, N. P., et al. (1986) · Journal of Medicinal Chemistry · doi:10.1021/jm00155a038 · Cited by 62 · see citing works
Interactions between neurons and glia in glutamate/glutamine compartmentation
Nicklas, W. J., Zeevalk, G. D., & Hyndman, A. G. (1987) · Biochemical Society Transactions · doi:10.1042/bst0150208 · Cited by 62 · see citing works
Electron Microscopic Study of Capillary Wall in Human Cerebral Edema
Castejón, O. J. (1980) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/00005072-198005000-00006 · Cited by 61 · see citing works
Ion transport processes in apical membranes of epithelia.
Dg, W., Greger, R. F., Pb, D., et al. (1984) · PubMed · Cited by 57 · see citing works
Garay, R. P., Hannaert, P. A., Nazaret, C., et al. (1986) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/bf00505823 · Cited by 55 · see citing works
Immunofluorescence study on the organization of actin in astroglial cells in primary cultures
Ciesielski-Treska, J., Guérold, B., & Aunis, D. (1982) · Neuroscience · doi:10.1016/0306-4522(82)90284-6 · Cited by 45 · see citing works
Jenkins, L. W., Becker, D. P., & Coburn, T. H. (1984) · Springer eBooks · doi:10.1007/978-1-4684-4616-6_51 · Cited by 37 · see citing works
Law, R. O. (1989) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1989.tb07328.x · Cited by 34 · see citing works
Shape, size, and distribution of cell structures by 3-D graphics reconstruction and stereology
Parsons, D. F., Cole, R. W., & Kimelberg, H. K. (1989) · Cell Biophysics · doi:10.1007/bf02797389 · Cited by 28 · see citing works
Self-citationUltrastructure of the central nervous system after prolonged hypoxia
Mang, C. Y., Bakay, L., & Lee, J. C. (1972) · Acta Neuropathologica · doi:10.1007/bf00684526 · Cited by 20 · see citing works
Free Amino Acid Content of Astroglial Cell Clones Derived from 8‐Day Postnatal Mouse Cerebella
Cambier, D., & Pessac, B. (1987) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1987.tb00964.x · Cited by 6 · see citing works
Treatment of tyramine-induced brain edema with anion transport inhibitor L-644, 711
Faraj, B. A., Cragoe, E. J., Sarper, R. M., et al. (1988) · Life Sciences · doi:10.1016/0024-3205(88)90341-4 · Cited by 5 · see citing works
THE ASTROCYTE IN LIVER DISEASE
(1971) · The Lancet · doi:10.1016/s0140-6736(71)90497-1 · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 6
- [24]24. Gilles, R., A. Kleinzeller, and L. Bolis, eds. (1987) Current Topics in Membranes and Transport, Vol. 30, Academic, New York.
- [33]33. Jenkins, L. W., D. P. Becker, and T. H. Cobum (1984) A quantitative analysis ofglial swelling and ischemic neuronal iniury following com- plete cerebral ischemia. In Recent Progress in the-study and Therapy of Brain Edema, K. G. Go and A. Baethmann, eds., pp. 523-537, Plenum, New York.
- [36]36. Kimelberg, H. K., and S. K. Goderie (1988) Volume regulation after swelling in primary astrocyte cultures. In Biochemical Pathology of Astrocytes, M. D. Norenberg and L. Hertz, eds., pp. 299-3 11, Liss, New York.
- [68]68. Wamock, D. G., R. Greger, P. B. Dunham, M. A. Benjamin, R. A. Frizzell, M. Field, K. R. Spring, H. E. Ives, P. S. Aronson, and J. Seifter (1983) Ion transport processes in apical membrane of epi- thelia. Fed. Proc. 43: 2473-2487.
- [69]69. Whetsell, W. O., Jr., C. Kohler, and R. Schwartz (1988) Quinolinic acid: A glia-derived excitotoxin in the mammalian central nervous system. In The BiochemicalPathology QfAstrocytes, M. D. Norenberg, L. Hertz, and A. Schousboe, eds., pp. 191-202, Liss, New York.
- [71]71. Yen, J. K., R. S. Bourke, A. J. Popp, and L. R. Nelson (1979) Use of ethacrynic acid in the treatment of serious head injury. In Neural Truuma, A. J. Popp, R. S. Bourke, L. R. Nelson, and H. K. Kimelberg, eds., pp. 329-339, Raven, New York.
Cited by
Sorted by citations
Tripartite synapses: glia, the unacknowledged partner
Araque, A., Parpura, V., Sanzgiri, R. P., & Haydon, P. G. (1999) · Trends in Neurosciences · doi:10.1016/s0166-2236(98)01349-6 · Cited by 2,529 · see citing works
Functional significance of cell volume regulatory mechanisms
LANG, F., BUSCH, G. L., RITTER, M., VÖLKL, H., WALDEGGER, S., GULBINS, E., & HÄUSSINGER, D. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.1.247 · Cited by 1,891 · see citing works
P2X <sub>7</sub> Receptor-Mediated Release of Excitatory Amino Acids from Astrocytes
Duan, S., Anderson, C. M., Keung, E. C., Chen, Y., Chen, Y., & Swanson, R. A. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-04-01320.2003 · Cited by 489 · see citing works
Beyond the role of glutamate as a neurotransmitter
Nedergaard, M., Takano, T., & Hansen, A. J. (2002) · Nature Reviews Neuroscience · doi:10.1038/nrn916 · Cited by 450 · see citing works
Ronaldson, P. T., & Davis, T. P. (2020) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x20951995 · Cited by 392 · see citing works
Astrocytes as secretory cells of the central nervous system: idiosyncrasies of vesicular secretion
Verkhratsky, A., Matteoli, M., Parpura, V., Mothet, J., & Zorec, R. (2016) · The EMBO Journal · doi:10.15252/embj.201592705 · Cited by 397 · see citing works
Bridges, R., Lutgen, V., Lobner, D., & Baker, D. A. (2012) · Pharmacological Reviews · doi:10.1124/pr.110.003889 · Cited by 205 · see citing works
Is high extracellular glutamate the key to excitotoxicity in traumatic brain injury?
Obrenovitch, T. P., & Urenjak, J. (1997) · Journal of Neurotrauma · doi:10.1089/neu.1997.14.677 · Cited by 205 · see citing works
Floyd, C. L., Gorin, F. A., & Lyeth, B. G. (2005) · Glia · doi:10.1002/glia.20183 · Cited by 121 · see citing works
The identification of a volume‐regulated anion channel: an amazing<scp>O</scp>dyssey
Pedersen, S. F., Klausen, T. K., & Nilius, B. (2015) · Acta Physiologica · doi:10.1111/apha.12450 · Cited by 115 · see citing works
Risher, W. C., Croom, D., & Kirov, S. A. (2012) · Glia · doi:10.1002/glia.22390 · Cited by 88 · see citing works
Sword, J., Masuda, T., Croom, D., & Kirov, S. A. (2013) · Brain · doi:10.1093/brain/awt026 · Cited by 81 · see citing works
GABA release from astrocytes in health and disease.
Kilb, W., & Kirischuk, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232415859 · Cited by 66 · see citing works
- Authors
- Kimelberg, H., Goderie, S., Higman, S., Pang, S., & Waniewski, R.
- Affiliations
- Albany Medical College (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1990-05-01
- Volume
- 10
- Issue
- 5
- Pages
- 1583-1591
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6570070
- OpenAlex
- W1936866440
- Semantic Scholar
- 46bd8998a1055c4586790474620f65570839ec5e
- Cited by
- 690
- References
- 57
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 12 · 2021: 14 · 2020: 12 · 2019: 9 · 2018: 14 · 2017: 12 · 2016: 16 · 2015: 16 · 2014: 15 · 2013: 15 · 2012: 19
- FWCI
- 9.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns021299-03, 5r01ns023750-06)
- National Institute of Neurological Disorders and Stroke (NS21299, NS-23750)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- astrocyte swelling, glutamate release, aspartate release, taurine release, hypotonic stress, glutamate uptake, anion transport inhibitors, membrane depolarization, astrocyte culture
- MeSH terms
- Amino Acids, Animals, Anions, Aspartic Acid, Astrocytes, Biological Transport, Cell Survival, Cells, Cultured, Culture Media, Cytochalasin B, Glutamates, Hypotonic Solutions, Sodium, Taurine, Glutamic Acid
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